FEI's direct electron detector developments: Embarking on a revolution in cryo-TEM

Journal of Structural Biology

Volumn 192, Issue 2, 2015, Pages 179-187

  Kuijper, Maarten ^a   van Hoften, Gerald ^a   Janssen, Bart ^a   Geurink, Rudolf ^a   De Carlo, Sacha ^a   Vos, Matthijn ^a   van Duinen, Gijs ^a   van Haeringen, Bart ^a   Storms, Marc ^a  

^a Thermo Fisher Scientific   (United States)

Author keywords

CMOS; Cryo EM; Direct electron detector; DQE; Electron counting; MTF; TEM

Indexed keywords

ANALYTICAL PARAMETERS; ARTICLE; CAMERA; CHEMICAL ANALYSIS; CONTROLLED STUDY; CRYO TRANSMISSION ELECTRON MICROSCOPY; DETECTION QUANTUM EFFICIENCY; DIRECT ELECTRON DETECTION; ELECTRON MICROSCOPY; FALCON CAMERA; FRACTIONATION; ILLUMINATION; IMAGE QUALITY; PRIORITY JOURNAL; SENSOR; SIGNAL NOISE RATIO; SIGNAL PROCESSING; THICKNESS; CRYOELECTRON MICROSCOPY; ELECTRON; PROCEDURES; THREE DIMENSIONAL IMAGING;

CRYOELECTRON MICROSCOPY; ELECTRONS; IMAGING, THREE-DIMENSIONAL;

EID: 84945911268     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2015.09.014     Document Type: Article

References (20)

1. Bai, X.C., Fernandez, I.S., McMullan, G., Scheres, S.H., 2013. Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles. eLife 2:e00461. Doi: 10.7554/eLife. 00461.
2. Baker L.A., Rubinstein J.L. Radiation damage in electron cryomicroscopy. Methods Enzymol. 2010, 481:371-388. 10.1016/S0076-6879(10)81015-8.
3. Campbell M.G., Cheng A., Brilot A.F., Moeller A., Lyumkis D., Veesler D., Pan J., Harrison S.C., Potter C.S., Carragher B., Griforieff N. Movies of ice-embedded particles enhance resolution in electron cryo-microscopy. Structure 2012, 20:1823-1828. 10.1016/j.str.2012.08.026.
4. Deptuch G., Besson A., Rehak P., Szelezniak M., Wall J., Winter M., Zhu Y. Direct electron imaging in electron microscopy with monolithic active pixel sensors. Ultramicroscopy 2007 Aug, 107(8):674-684.
5. Faruqi A.R., Cattermole D.M., Henderson R., Mikulec B., Raeburn C. Evaluation of a hybrid pixel detector for electron microscopy. Ultramicroscopy 2003, 94:263-276.
6. Guerrini N., Turchetta R., van Hoften G., Henderson R., McMullan G., Faruqi A.R. A high frame rate, 16 million pixels, radiation hard CMOS sensor. J. Instrum. 2011.
7. Henderson R. Image contrast in high-resolution electron microscopy of biological macromolecules: TMV in ice. Ultramicroscopy 1992, 46:1-18.
8. http://www.microscopy-analysis.com/sites/default/files/2013_Sept_Booth.pdf.
9. https://en.wikipedia.org/wiki/Finite_element_method.
10. ISO12233.
11. Li X., Mooney P., Zheng S., Booth C.R., Braunfeld M.B., Gubbens S., Agard D.A., Cheng Y. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 2013, 10(6):584-590.
12. McMullan G., Chen S., Henderson R., Faruqi A.R. Detective quantum efficiency of electron area detectors in electron microscopy. Ultramicroscopy 2009, 109:1126-1143.
13. McMullan G., Clare D., Turchetta R., Faruqi A.R. Enhanced imaging in low dose electron microscopy using electron counting. Ultramicroscopy 2009, 109:1411-1416.
14. McMullan G., Faruqi A.R., Clare D., Henderson R. Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy. Ultramicroscopy 2014, 147:156-163.
15. Meyer R.R., Kirkland A.I. Characterisation of the signal and noise transfer of CCD cameras for electron detection. Microsc. Res. Tech. 2000 May 1, 49(3):269-280.
16. Milazzo A.C., Leblanc P., Duttweiler F., Jin L., Bouwer J.C., Peltier S., Ellisman M., Bieser F., Matis H.S., Wieman H., Denes P., Kleinfelder S., Xuong N.H. Active pixel sensor array as a detector for electron microscopy. Ultramicroscopy 2005, 104:152-159.
17. Ruskin R.S., Yu Z., Grigorieff N. Quantitative characterization of electron detectors for transmission electron microscopy. J. Struct. Biol. 2013, 184(3):385-393.
18. Samei E., Flynn M.J., Reimann D.A. A method for measuring the presampled MTF of digital radiographic systems using an edge test device. Med. Phys. 1998, 25:102-113.
19. Scheres, S.H.W., 2014. Beam-induced motion correction for sub-megadalton cryo-EM particles. eLife 3:e03665. Doi: 10.7554/eLife. 03665.
20. Sousa D., Grigorieff N. Ab initio resolution measurement for single particle structures. J. Struct. Biol. 2006, 157:201-210.