Engineering the Organophosphorus Acid Anhydrolase Enzyme for Increased Catalytic Efficiency and Broadened Stereospecificity on Russian VX

Biochemistry

Volumn 54, Issue 41, 2015, Pages 6423-6433

  Daczkowski, Courtney M ^a   Pegan, Scott D ^a,b   Harvey, Steven P ^c  

^a UNIVERSITY OF GEORGIA   (United States)

^b US Army Reserve 377th Sustainment Command Detachment 8   (United States)

^c EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING SITES; CHEMICAL COMPOUNDS; CLONING; EFFICIENCY; ENANTIOMERS; ENZYMES; ESCHERICHIA COLI; PHOSPHORUS COMPOUNDS; PROTEINS;

CATALYTIC EFFICIENCIES; CHEMICAL NERVE AGENTS; GENETIC BACKGROUNDS; ORGANOPHOSPHORUS ACIDS; ORGANOPHOSPHORUS COMPOUNDS; PROTEIN ENGINEERING; STEREOSPECIFICITY; SUBSTRATE-BINDING SITES;

BIOCHEMICAL ENGINEERING;

HYDROLASE; ORGANOPHOSPHORUS COMPOUND; PHOSPHORUS ACID DERIVATIVE; ARYLDIALKYLPHOSPHATASE; PHOSPHOROTHIOIC ACID DERIVATIVE; S-(N,N-DIETHYLAMINOETHYL) ISOBUTYL METHYLPHOSPHOTHIOLATE;

ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CRYSTALLIZATION; ENANTIOMER; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME ENGINEERING; EXPRESSION VECTOR; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY; ALTEROMONAS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STEREOISOMERISM; X RAY CRYSTALLOGRAPHY;

ALTEROMONAS; ARYLDIALKYLPHOSPHATASE; CATALYTIC DOMAIN; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; ORGANOTHIOPHOSPHORUS COMPOUNDS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 84945576042     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.5b00624     Document Type: Article

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