3D scaffold alters cellular response to graphene in a polymer composite for orthopedic applications

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Volumn 104, Issue 4, 2016, Pages 732-749

  Kumar, Sachin ^a   Azam, Dilkash ^a   Raj, Shammy ^a   Kolanthai, Elayaraja ^a   Vasu, K S ^a   Sood, A K ^a   Chatterjee, Kaushik ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

bone tissue engineering; graphene; nanotoxicology; osteoblasts; polymer nanocomposites

Indexed keywords

CELL PROLIFERATION; CELLS; COMPOSITE MATERIALS; CYTOLOGY; FRACTURE FIXATION; FUNCTIONAL POLYMERS; GRAPHENE; MEDICAL APPLICATIONS; NANOCOMPOSITES; NANOPARTICLES; OSTEOBLASTS; POLYCAPROLACTONE; POLYMERS; TISSUE; TISSUE ENGINEERING;

BIOMEDICAL APPLICATIONS; BONE TISSUE ENGINEERING; NANOTOXICOLOGY; ORTHOPEDIC APPLICATIONS; POLYMER NANOCOMPOSITE; REDUCED GRAPHENE OXIDES (RGO); THREE-DIMENSIONAL (3D) SCAFFOLDS; TWO DIMENSIONAL (2 D);

SCAFFOLDS (BIOLOGY);

GRAPHENE; GRAPHENE OXIDE; NANOCOMPOSITE; POLYCAPROLACTONE; POLYMER; BONE PROSTHESIS; GRAPHITE; POLYESTER;

ANIMAL CELL; ATOMIC FORCE MICROSCOPY; CELL ADHESION; CELL CONTACT; CELL PROLIFERATION; COMPARATIVE STUDY; CONFERENCE PAPER; CONTACT ANGLE; CRYSTALLIZATION; DNA CONTENT; FRACTURE FIXATION; IN VITRO STUDY; INFRARED SPECTROSCOPY; MICRO-COMPUTED TOMOGRAPHY; MOUSE; NONHUMAN; ORTHOPEDIC IMPLANT; OSTEOBLAST; POTENTIAL DIFFERENCE; RAMAN SPECTROMETRY; SCANNING ELECTRON MICROSCOPY; SURFACE PROPERTY; TISSUE SCAFFOLD; WETTABILITY; X RAY DIFFRACTION; ANIMAL; BONE PROSTHESIS; CELL LINE; CHEMISTRY; CYTOLOGY; METABOLISM; POROSITY;

ANIMALS; BONE SUBSTITUTES; CELL LINE; FRACTURE FIXATION; GRAPHITE; MICE; NANOCOMPOSITES; OSTEOBLASTS; POLYESTERS; POROSITY; TISSUE SCAFFOLDS;

EID: 84944519325     PISSN: 15524973     EISSN: 15524981     Source Type: Journal    
DOI: 10.1002/jbm.b.33549     Document Type: Conference Paper

References (62)

1. Feng L, Liu Z., Graphene in biomedicine: Opportunities and challenges. Nanomedicine 2011; 6: 317-324.
2. Park SY, Park J, Sim SH, Sung MG, Kim KS, Hong BH, Hong S., Enhanced differentiation of human neural stem cells into neurons on graphene. Adv Mater 2011; 23: H263-H267.
3. Qi Y, Tai Z, Sun D, Chen J, Ma H, Yan X, Liu B, Xue Q., Fabrication and characterization of poly (vinyl alcohol)/graphene oxide nanofibrous biocomposite scaffolds. J Appl Polym Sci 2013; 127: 1885-1894.
4. Zhang J, Qiu Z., Morphology, crystallization behavior, and dynamic mechanical properties of biodegradable poly (ϵ-caprolactone)/thermally reduced graphene nanocomposites. Ind Eng Chem Res 2011; 50: 13885-13891.
5. Kolanthai E, Kalsar R, Bose S, Suwas S, Chatterjee K., Combinatorial effect of rolling and carbonaceous nanoparticles on the evolution of crystallographic texture and structural properties of ultra high molecular weight polyethylene. Phys Chem Chem Phys 2014; 16: 23108-23117.
6. Sarkar K, Madras G, Chatterjee K., Dendron conjugation to graphene oxide using click chemistry for efficient gene delivery. RSC Adv 2015; 5: 50196-50211.
7. Liao K-H, Lin Y-S, Macosko CW, Haynes CL., Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. ACS Appl Mater Interfaces 2011; 3: 2607-2615.
8. Li N, Zhang X, Song Q, Su R, Zhang Q, Kong T, Liu L, Jin G, Tang M, Cheng G., The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates. Biomaterials 2011; 32: 9374-9382.
9. Yang K, Li Y, Tan X, Peng R, Liu Z., Behavior and toxicity of graphene and its functionalized derivatives in biological systems. Small 2012; 9: 1492-1503.
10. Cukierman E, Pankov R, Stevens DR, Yamada KM., Taking cell-matrix adhesions to the third dimension. Science 2001; 294: 1708-1712.
11. Hall HG, Farson DA, Bissell MJ., Lumen formation by epithelial cell lines in response to collagen overlay: A morphogenetic model in culture. Proc Natl Acad Sci USA 1982; 79: 4672-4676.
12. Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, Wilkinson CD, Oreffo RO., The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater 2007; 6: 997-1003.
13. Kumar G, Tison CK, Chatterjee K, Pine PS, McDaniel JH, Salit ML, Young MF, Simon CG Jr,. The determination of stem cell fate by 3D scaffold structures through the control of cell shape. Biomaterials 2011; 32: 9188-9196.
14. Lee J, Lilly GD, Doty RC, Podsiadlo P, Kotov NA., In vitro toxicity testing of nanoparticles in 3D cell culture. Small 2009; 5: 1213-1221.
15. Mansfield E, Oreskovic TL, Rentz NS, Jeerage KM., Three-dimensional hydrogel constructs for exposing cells to nanoparticles. Nanotoxicology 2014; 8: 394-403.
16. Movia D, Prina-Mello A, Bazou D, Volkov Y, Giordani S., Screening the cytotoxicity of single-walled carbon nanotubes using novel 3D tissue-mimetic models. ACS Nano 2011; 5: 9278-9290.
17. Park S, Ruoff RS., Chemical methods for the production of graphenes. Nat Nanotechnol 2009; 4: 217-224.
18. He H, Riedl T, Lerf A, Klinowski J., Solid-state NMR studies of the structure of graphite oxide. J Phys Chem 1996; 100: 19954-19958.
19. Fan H, Wang L, Zhao K, Li N, Shi Z, Ge Z, Jin Z., Fabrication, mechanical properties, and biocompatibility of graphene-reinforced chitosan composites. Biomacromolecules 2010; 11: 2345-2351.
20. Wan C, Chen B., Poly (ϵ-caprolactone)/graphene oxide biocomposites: Mechanical properties and bioactivity. Biomed Mater 2011; 6: 055010.
21. Sayyar S, Murray E, Thompson BC, Gambhir S, Officer DL, Wallace GG., Covalently linked biocompatible graphene/polycaprolactone composites for tissue engineering. Carbon 2013; 52: 296-304.
22. Girase B, Shah JS, Misra RDK., Cellular mechanics of modulated osteoblasts functions in graphene oxide reinforced elastomers. Adv Eng Mater 2012; 14: B101-B111.
23. Kumar S, Chatterjee K., Strontium eluting graphene hybrid nanoparticles augment osteogenesis in a 3D tissue scaffold. Nanoscale 2015; 7: 2023-2033.
24. Kai W, Hirota Y, Hua L, Inoue Y., Thermal and mechanical properties of a poly (-caprolactone)/graphite oxide composite. J Appl Polym Sci 2008; 107: 1395-1400.
25. Chang Y, Yang S-T, Liu J-H, Dong E, Wang Y, Cao A, Liu Y, Wang H., In vitro toxicity evaluation of graphene oxide on A549 cells. Toxicol Lett 2011; 200: 201-210.
26. Hummers WS Jr, Offeman RE., Preparation of graphitic oxide. J Am Chem Soc 1958; 80: 1339-1339.
27. Chatterjee K, Sun L, Chow LC, Young MF, Simon CG Jr,. Combinatorial screening of osteoblast response to 3D calcium phosphate/poly (ϵ-caprolactone) scaffolds using gradients and arrays. Biomaterials 2011; 32: 1361-1369.
28. Marszalek JE, Simon CG, Thodeti C, Adapala RK, Murthy A, Karim A., 2.5 D constructs for characterizing phase separated polymer blend surface morphology in tissue engineering scaffolds. J Biomed Mater Res A 2013; 101: 1502-1510.
29. Chatterjee K, Hung S, Kumar G, Simon CG., Time-dependent effects of pre-aging 3D polymer scaffolds in cell culture medium on cell proliferation. J Funct Biomater 2012; 3: 372-381.
30. Bi Y, Stuelten CH, Kilts T, Wadhwa S, Iozzo RV, Robey PG, Chen X-D, Young MF., Extracellular matrix proteoglycans control the fate of bone marrow stromal cells. J Biol Chem 2005; 280: 30481-30489.
31. Jeong H-K, Lee YP, Lahaye RJ, Park M-H, An KH, Kim IJ, Yang C-W, Park CY, Ruoff RS, Lee YH., Evidence of graphitic AB stacking order of graphite oxides. J Am Chem Soc 2008; 130: 1362-1366.
32. Gudarzi MM, Sharif F., Enhancement of dispersion and bonding of graphene-polymer through wet transfer of functionalized graphene oxide. eXPRESS Polym Lett 2012; 6: 1017-1031.
33. Hae-Mi Ju, Sung-Ho Choi, Huh SH., X-ray diffraction patterns of thermally-reduced graphenes. J Korean Phys Soc 2010; 57: 1649-1652.
34. Kim H, Abdala AA, Macosko CW., Graphene/polymer nanocomposites. Macromolecules 2010; 43: 6515-6530.
35. Yang Y, Rigdon W, Huang X, Li X., Enhancing graphene reinforcing potential in composites by hydrogen passivation induced dispersion. Sci Rep 2013; 3: 2086.
36. Zhu Y, Stoller MD, Cai W, Velamakanni A, Piner RD, Chen D, Ruoff RS., Exfoliation of graphite oxide in propylene carbonate and thermal reduction of the resulting graphene oxide platelets. ACS Nano 2010; 4: 1227-1233.
37. Nemanich R, Solin S., First-and second-order Raman scattering from finite-size crystals of graphite. Phys Rev B 1979; 20: 392.
38. Chen C, Long M, Xia M, Zhang C, Cai W., Reduction of graphene oxide by an in-situ photoelectrochemical method in a dye-sensitized solar cell assembly. Nanoscale Res Lett 2012; 7: 1-5.
39. Ahn HS, Jang J-W, Seol M, Kim JM, Yun D-J, Park C, Kim H, Youn DH, Kim JY, Park G., Self-assembled foam-like graphene networks formed through nucleate boiling. Sci Rep 2013; 3: 1-8.
40. Zhao Y, Zhan L, Tian J, Nie S, Ning Z., Enhanced electrocatalytic oxidation of methanol on Pd/polypyrrole-graphene in alkaline medium. Electrochim Acta 2011; 56: 1967-1972.
41. Guo Y, Sun X, Liu Y, Wang W, Qiu H, Gao J., One pot preparation of reduced graphene oxide (RGO) or Au (Ag) nanoparticle-RGO hybrids using chitosan as a reducing and stabilizing agent and their use in methanol electrooxidation. Carbon 2012; 50: 2513-2523.
42. Wang K, Li W, Gao C., Poly (-caprolactone) functionalized carbon nanofibers by surface initiated ring opening polymerization. J Appl Polym Sci 2007; 105: 629-640.
43. Cullity BD., Structure of polycrystalline aggregates: Elements of X-ray diffraction, Addison-Wesley Publishing Company, 2nd edition, 1978; 284-285.
44. Messersmith PB, Giannelis EP., Synthesis and barrier properties of poly (ϵ-caprolactone)-layered silicate nanocomposites. J Polym Sci A Polym Chem 1995; 33: 1047-1057.
45. Sayyar BS, Murray E, Thompson BC, Gambhir S, Officer DL, Wallace GG., Covalently linked biocompatible graphene/polycaprolactone composites for tissue engineering. Carbon 2013; 52: 296-304.
46. Hua L, Kai WH, Inoue Y., Crystallization behavior of poly (caprolactone)/graphite oxide composites. J Appl Polym Sci 2007; 106: 4225-4232.
47. Cai D, Song M., A simple route to enhance the interface between graphite oxide nanoplatelets and a semi-crystalline polymer for stress transfer. Nanotechnology 2009; 20: 315708.
48. Lahiri D, Dua R, Zhang C, de Socarraz-Novoa I, Bhat A, Ramaswamy S, Agarwal A., Graphene nanoplatelet-induced strengthening of ultrahigh molecular weight polyethylene and biocompatibility in vitro. ACS Appl Mater Interfaces 2012; 4: 2234-2241.
49. Cheng K, Lai Y, Kisaalita WS., Three-dimensional polymer scaffolds for high throughput cell-based assay systems. Biomaterials 2008; 29: 2802-2812.
50. Ge C, Li Y, Yin J-J, Liu Y, Wang L, Zhao Y, Chen C., The contributions of metal impurities and tube structure to the toxicity of carbon nanotube materials. NPG Asia Mater 2012; 4: e32.
51. Ma P-C, Mo S-Y, Tang B-Z, Kim J-K., Dispersion, interfacial interaction and re-agglomeration of functionalized carbon nanotubes in epoxy composites. Carbon 2010; 48: 1824-1834.
52. Cheng Z, Teoh S-H., Surface modification of ultra thin poly (ϵ-caprolactone) films using acrylic acid and collagen. Biomaterials 2004; 25: 1991-2001.
53. Tiaw K, Goh S, Hong M, Wang Z, Lan B, Teoh S., Laser surface modification of poly (ϵ-caprolactone)(PCL) membrane for tissue engineering applications. Biomaterials 2005; 26: 763-769.
54. Wang S, Zhang Y, Abidi N, Cabrales L., Wettability and surface free energy of graphene films. Langmuir 2009; 25: 11078-11081.
55. Wei J, Igarashi T, Okumori N, Igarashi T, Maetani T, Liu B, Yoshinari M., Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts. Biomed Mater 2009; 4: 045002.
56. Vogler EA., Structure and reactivity of water at biomaterial surfaces. Adv Colloid Interface Sci 1998; 74: 69-117.
57. Lampin M, Warocquier-Clérout R, Legris C, Degrange M, Sigot-Luizard M., Correlation between substratum roughness and wettability, cell adhesion, and cell migration. J Biomed Mater Res 1997; 36: 99-108.
58. Depan D, Girase B, Shah J, Misra R., Structure-process-property relationship of the polar graphene oxide-mediated cellular response and stimulated growth of osteoblasts on hybrid chitosan network structure nanocomposite scaffolds. Acta Biomater 2011; 7: 3432-3445.
59. Lee WC, Lim CHY, Shi H, Tang LA, Wang Y, Lim CT, Loh KP., Origin of enhanced stem cell growth and differentiation on graphene and graphene oxide. ACS Nano 2011; 5: 7334-7341.
60. Kumar S, Raj S, Kolanthai E, Sood AK, Sampath S, Chatterjee K., Chemical functionalization of graphene to augment stem cell osteogenesis and inhibit biofilm formation on polymer composites for orthopedic applications. ACS Appl Mater Interfaces 2015; 7: 3237-3252.
61. Farooque TM, Camp CH, Tison CK, Kumar G, Parekh SH, Simon CG., Measuring stem cell dimensionality in tissue scaffolds. Biomaterials 2014; 35: 2558-2567.
62. Riccio M, Resca E, Maraldi T, Pisciotta A, Ferrari A, Bruzzesi G, De Pol A., Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures. Eur J Histochem 2010; 54: 213-221.