New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters

Human Vaccines and Immunotherapeutics

Volumn 11, Issue 7, 2015, Pages 1865-1871

  Fernandes Monteiro, Alice G ^a,b   Trindade, Gisela F ^a   Yamamura, Anna M Y ^a   Moreira, Otacilio C ^b   de Paula, Vanessa S ^b   Duarte, Ana Cláudia M ^a   Britto, Constança ^b   Lima, Sheila Maria B ^a  

^a Instituto de Technologia em Imunobiologicos   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Molecular diagnosis; RT qPCR; Viral load; Viral vaccines; Yellow fever virus

Indexed keywords

NONSTRUCTURAL PROTEIN 5; VIRUS RNA; YELLOW FEVER VACCINE;

ANALYTICAL PARAMETERS; ARTICLE; CONTROLLED STUDY; COPY NUMBER VARIATION; DENGUE VIRUS; HUMAN; JAPANESE ENCEPHALITIS VIRUS; LINEARITY; MEASLES VIRUS; MEASUREMENT PRECISION; MOLECULAR PROBE; NONHUMAN; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SPECIFICITY; STANDARDIZATION; VALIDATION STUDY; VIRUS LOAD; VIRUS TITRATION; YELLOW FEVER VIRUS; ANIMAL; ANTIBODY SPECIFICITY; CHLOROCEBUS AETHIOPS; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; QUALITY CONTROL; REPRODUCIBILITY; STANDARD; STANDARDS; VERO CELL LINE; VIREMIA; VIROLOGY; YELLOW FEVER;

ANIMALS; ANTIBODY SPECIFICITY; CERCOPITHECUS AETHIOPS; HUMANS; PLASMIDS; QUALITY CONTROL; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; VERO CELLS; VIRAL LOAD; VIREMIA; YELLOW FEVER; YELLOW FEVER VACCINE; YELLOW FEVER VIRUS;

EID: 84944445955     PISSN: 21645515     EISSN: 2164554X     Source Type: Journal    
DOI: 10.4161/21645515.2014.990854     Document Type: Article

References (21)

1. Monath TP. Pathobiology of Flaviviruses. "Togaviridae and the Flaviviridae". Schlesinger S, Schlesinger MJ, eds. New York: Plenum Press; 1986:375-440.
2. Chambers TJ, Hahn CS, Galler R, Rice CM. Flavivirus genome organisation, expression and replication. Annu Rev Microbiol 1990; 44:649-88; PMID:2174669; http://dx. doi. org/10. 1146/annurev. mi. 44. 100190. 003245
3. Lindenbach BD, Rice CM. Flaviviridae: The viruses and their replication. Philadelphia: LippincottWilliams & Wilkins; 2001. 991-1042 (Knipe DM, Howley PM, editors. Fields Virology; vol. 1)
4. Brinton MA. The molecular biology of West Nile Virus: a new invader of the western hemisphere. Annu Rev Microbiol 2002; 56:371-402; PMID:12142476; http://dx. doi. org/10. 1146/annurev. micro. 56. 012302. 160654
5. Burke DS, Monath TP. Flaviviruses. Philadelphia: Lippincott, Williams & Wilkins; 2001. 1043-1125 (Knipe DM, Howley PM, editors. Fields Virology; vol. 1)
6. Martins RM, Maia MD, Farias RH, Camacho LA, Freire MS, Galler R, Yamamura AM, Almeida LF, Lima SM, Nogueira RM, Sá GR, et al. 17DD yellow fever vaccine: a double blind, randomized clinical trial of immunogenicity and safety on a dose-response study. Hum Vaccin Immunother 2013:30-9; PMID: 23364472; http://dx. doi. org/10. 4161/hv. 22982
7. Deubel V, Huerre M, Cathomas G, Drouet MT, Wuscher N, LeGuenno B, Widmer AF. Molecular detection and characterization of yellow fever virus in blood and liver specimens of a non-vaccinated fatal human case. J Med Virol 1997; 53:212-7; PMID:9365884; http://dx. doi. org/10. 1002/(SICI)1096-9071(199711)53:3%3c 212::AID-JMV5%3e3. 0. CO;2-B
8. De Brito T, Siqueira SA, Santos RT, Nassar ES, Coimbra TL, Alves VA. Human fatal yellow fever. Immunohistochemical detection of viral antigens in the liver, kidney and heart. Pathol Res Pract 1992; 188:177-81; PMID:1594489; http://dx. doi. org/10. 1016/S0344- 0338(11)81176-3
9. Monath TP, Ballinger ME, Miller BR, Salaun JJ. Detection of yellow fever viral RNA by nucleic acid hybridization and viral antigen by immunocytochemistry in fixed human liver. Am J Trop Med Hyg 1989; 40:663-8; PMID:2500857
10. Monath TP, Nystrom RR. Detection of yellow fever virus in serum by enzyme immunoassay. Am J Trop Med Hyg 1984; 33:151-7; PMID:6364854
11. Porterfield JS. A plaque technique for the titration of yellow fever virus and antisera. Trans R Soc Trop Med Hyg 1959; 53:458-65; PMID:14434289; http://dx. doi. org/10. 1016/0035-9203(59)90021-5
12. Mackay IM, Arden KE, Nitsche A. Real-Time PCR in virology. Nucleic Acids Res 2002; 30:1292-305; PMID:11884626; http://dx. doi. org/10. 1093/nar/30. 6. 1292
13. Manojkumar R, Mrudula V. Applications of Real- Time reverse transcription polymerase chain reaction in clinical virology laboratories for the diagnosis of human diseases. Am J Infectious Dis 2006; 2:204-9; http://dx. doi. org/10. 3844/ajidsp. 2006. 204. 209
14. ANVISA, 2003 http://www. anvisa. gov, br/legis/resol/ 2003/re/898_03re. htm (ANVISA - Agência Nacional de Vigilância Sanit_aria, 2003. Accessed in July 24, 2012)
15. Barbana V, Girerda Y, Aguirrea M, Guliaa S, Pétiarda F, Rioub P, Barrereb B, Langa J. High stability of yellow fever 17D-204 vaccine: A 12-year restrospective analysis of 11 large-scale production. Vaccine 2007; 25:2941-50; PMID:16914238; http://dx. doi. org/ 10. 1016/j. vaccine. 2006. 06. 082
16. Bae HG, Nitsche A, Teichmann A, Biel SS, Niedrig M. Detection of yellow fever virus: a comparison of quantitative Real-Time PCR and plaque assay. J Virol Methods 2003; 110:185-91; PMID:12798247; http://dx. doi. org/10. 1016/S0166-0934(03)00129-0
17. Mantel N, Aguirre M, Gulia S, Girerd-Chambaz Y, Colombani S, Moste C, Barban V. Standardized quantitative RT-PCR assays for quantitation of yellow fever and chimeric yellow fever-dengue vaccines. J Virol Methods 2008; 151:40-6; PMID:18501437; http://dx. doi. org/10. 1016/j. jviromet. 2008. 03. 026
18. Gurukumar KR, Priyadarshini D, Patil JA, Bhagat A, Singh A, Shah PS, Cecilia D. Development of real time PCR for detection and quantitation of Dengue Viruses. Virol J 2009; 23:6-10; PMID:19166574; http://dx. doi. org/10. 1186/1743-422X-6-10
19. da Cunha RV, Dias M, Nogueira RMR, Chagas N, Miagostovich MP, Schatzmayr H. Secondary dengue infection in school childrens in a dengue endemic área in the state of Rio de Janeiro, Brazil. Rev Inst Med Trop S Paulo 1995; 37:517-21; PMID:8731265; http://dx. doi. org/ 10. 1590/S0036-46651995000600008
20. Sloan LM. Real-Time PCR in clinical microbiology: verification, validation, and contamination control. Clin Microbiol Newsletter 2007; 29; http://dx. doi. org/ 10. 1016/j. clinmicnews. 2007. 05. 002
21. Burd EM. Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 2010; 23:550-76; PMID:20610823; http://dx. doi. org/ 10. 1128/CMR. 00074-09