-
1
-
-
0025033985
-
Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast
-
J. Aikawa, T. Yamashita, M. Nishiyama, S. Horinouchi and T. Beppu (1990) Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast. J. Biol. Chem. 265 13955-13959.
-
(1990)
J. Biol. Chem.
, vol.265
, pp. 13955-13959
-
-
Aikawa, J.1
Yamashita, T.2
Nishiyama, M.3
Horinouchi, S.4
Beppu, T.5
-
2
-
-
0034986675
-
Replacements of amino acid residues at subsites and their effects on the catalytic properties of Rhizomucor pusillus pepsin, an aspartic proteinase from Rhizomucor pusillus
-
J. Aikawa, Y.N. Park, M. Sugiyama, M. Nishiyama, S. Horinouchi and T. Beppu (2001) Replacements of amino acid residues at subsites and their effects on the catalytic properties of Rhizomucor pusillus pepsin, an aspartic proteinase from Rhizomucor pusillus. J. Biochem. 129 791-794.
-
(2001)
J. Biochem.
, vol.129
, pp. 791-794
-
-
Aikawa, J.1
Park, Y.N.2
Sugiyama, M.3
Nishiyama, M.4
Horinouchi, S.5
Beppu, T.6
-
3
-
-
85012738381
-
Estimation of pepsin, papain and cathepsin with haemoglobin, J
-
M.I. Anson (1938) Estimation of pepsin, papain and cathepsin with haemoglobin, J. Gen. Physiol. 22 79-89.
-
(1938)
Gen. Physiol.
, vol.22
, pp. 79-89
-
-
Anson, M.I.1
-
4
-
-
85007860919
-
Milk clotting enzyme from microorganisms. Part I. Screening test and the identification of the potent fungus
-
K. Arima, S. Iwasaki and G. Tamura (1967) Milk clotting enzyme from microorganisms. Part I. Screening test and the identification of the potent fungus. Agric. Biol. Chem. 31 540-545.
-
(1967)
Agric. Biol. Chem.
, vol.31
, pp. 540-545
-
-
Arima, K.1
Iwasaki, S.2
Tamura, G.3
-
5
-
-
0001442231
-
Milk-clotting enzyme from Mucor pusillus var. Lindt
-
K. Arima, J. Yu and S. Iwasaki (1970) Milk-clotting enzyme from Mucor pusillus var. Lindt. Methods Enzymol. 19 446-459.
-
(1970)
Methods Enzymol.
, vol.19
, pp. 446-459
-
-
Arima, K.1
Yu, J.2
Iwasaki, S.3
-
6
-
-
84884869135
-
Tyrosine 75 on the flap contributes to enhance catalytic efficiency of a fungal aspartic proteinase, Mucor pusillus pepsin
-
T. Takahashi (Ed.), New York: Plenum Press
-
T. Beppu, Y.-N. Park, J. Aikawa, M. Nishiyama and S. Horinouchi (1995) Tyrosine 75 on the flap contributes to enhance catalytic efficiency of a fungal aspartic proteinase, Mucor pusillus pepsin. T. Takahashi (Ed.) Aspartic Proteinase: Structure, Function, Biology, and Biomedical Implications New York: Plenum Press 559-563.
-
(1995)
Aspartic Proteinase: Structure, Function, Biology, and Biomedical Implications
, pp. 559-563
-
-
Beppu, T.1
Park, Y.-N.2
Aikawa, J.3
Nishiyama, M.4
Horinouchi, S.5
-
7
-
-
0022831764
-
Primary structure of a precursor to the aspartic proteinase from Rhizomucor miehei shows that the enzyme is synthesized as a zymogen
-
E. Boel, A.M. Bech, K. Randrup, B. Draeger, N.P. Fiil and B. Foltmann (1986) Primary structure of a precursor to the aspartic proteinase from Rhizomucor miehei shows that the enzyme is synthesized as a zymogen. Proteins 1 363-369.
-
(1986)
Proteins
, vol.1
, pp. 363-369
-
-
Boel, E.1
Bech, A.M.2
Randrup, K.3
Draeger, B.4
Fiil, N.P.5
Foltmann, B.6
-
8
-
-
0024267317
-
High-level expression of recombinant genes in Aspergillus oryzae
-
T. Christensen, H. Woeldike, E. Boel, S.B. Mortensen, K. Hjortshoej, L. Thim and M.T. Hansen (1988) High-level expression of recombinant genes in Aspergillus oryzae. Bio/Technology 6 1419-1422.
-
(1988)
Bio/Technology
, vol.6
, pp. 1419-1422
-
-
Christensen, T.1
Woeldike, H.2
Boel, E.3
Mortensen, S.B.4
Hjortshoej, K.5
Thim, L.6
Hansen, M.T.7
-
9
-
-
0008011162
-
Physicochemical and immunochemical studies on similarities of acid proteases Mucor pusillus rennin and Mucor miehei rennin
-
Y. Etoh, H. Shoun, T. Beppu and K. Arima (1979) Physicochemical and immunochemical studies on similarities of acid proteases Mucor pusillus rennin and Mucor miehei rennin. Agric. Biol. Chem. 43 209-215.
-
(1979)
Agric. Biol. Chem.
, vol.43
, pp. 209-215
-
-
Etoh, Y.1
Shoun, H.2
Beppu, T.3
Arima, K.4
-
10
-
-
0022919725
-
Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate
-
G.L. Gray, K. Hayenga, D. Cullen, L.J. Wilson and S. Norton (1986) Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate. Gene 48 41-53.
-
(1986)
Gene
, vol.48
, pp. 41-53
-
-
Gray, G.L.1
Hayenga, K.2
Cullen, D.3
Wilson, L.J.4
Norton, S.5
-
11
-
-
0024424630
-
Secretion by yeast of the zymogen form of Mucor rennin, an aspartic proteinase of Mucor pusillus, and its conversion to the mature form
-
R. Hiramatsu, J. Aikawa, S. Horinouchi and T. Beppu (1989) Secretion by yeast of the zymogen form of Mucor rennin, an aspartic proteinase of Mucor pusillus, and its conversion to the mature form. J. Biol. Chem. 264 16862-16866.
-
(1989)
J. Biol. Chem.
, vol.264
, pp. 16862-16866
-
-
Hiramatsu, R.1
Aikawa, J.2
Horinouchi, S.3
Beppu, T.4
-
12
-
-
85007778652
-
Milk clotting enzyme from microorganisms. Part II. The enzyme production and the properties of crude enzyme
-
S. Iwasaki, G. Tamura and K. Arima (1967) Milk clotting enzyme from microorganisms. Part II. The enzyme production and the properties of crude enzyme. Agric. Biol. Chem. 31 546-551.
-
(1967)
Agric. Biol. Chem.
, vol.31
, pp. 546-551
-
-
Iwasaki, S.1
Tamura, G.2
Arima, K.3
-
13
-
-
0019322201
-
Kinetic studies on the action of Mucor pusillus, Mucor miehei acid proteases and chymosins A and B on a synthetic chromophoric hexapeptide
-
P. Martin, M.-N. Raymond, E. Bricas and B. Ribadeau Ducas (1980) Kinetic studies on the action of Mucor pusillus, Mucor miehei acid proteases and chymosins A and B on a synthetic chromophoric hexapeptide. Biochim. Biophys. Acta 612 410-420.
-
(1980)
Biochim. Biophys. Acta
, vol.612
, pp. 410-420
-
-
Martin, P.1
Raymond, M.-N.2
Bricas, E.3
Ribadeau Ducas, B.4
-
14
-
-
0027378992
-
Characterization of an aspartic proteinase of Mucor pusillus expressed in Aspergillus oryzae
-
K. Murakami, J. Aikawa, S. Horinouchi and T. Beppu (1993) Characterization of an aspartic proteinase of Mucor pusillus expressed in Aspergillus oryzae. Mol. Gen Genet. 241 312-318.
-
(1993)
Mol. Gen Genet.
, vol.241
, pp. 312-318
-
-
Murakami, K.1
Aikawa, J.2
Horinouchi, S.3
Beppu, T.4
-
15
-
-
0027446891
-
X-ray analyses of aspartic proteinases. V. Structure and refinement at 2.0 Å resolution of the aspartic proteinase from Mucor pusillus
-
M. Newman, F. Watson, P. Roychowdhury, H. Jones, M. Badasso, A. Cleasby, S.P. Wood, I.J. Tickle and T.L. Blundell (1993) X-ray analyses of aspartic proteinases. V. Structure and refinement at 2.0 Å resolution of the aspartic proteinase from Mucor pusillus. J. Mol. Biol. 230 260-283.
-
(1993)
J. Mol. Biol.
, vol.230
, pp. 260-283
-
-
Newman, M.1
Watson, F.2
Roychowdhury, P.3
Jones, H.4
Badasso, M.5
Cleasby, A.6
Wood, S.P.7
Tickle, I.J.8
Blundell, T.L.9
-
16
-
-
0015859266
-
On the specificity of a rennin-like enzyme from Mucor pusillus
-
T. Oka, K. Ishino, H. Tsuzuki, K. Morihara and K. Arima (1973) On the specificity of a rennin-like enzyme from Mucor pusillus. Agric. Biol. Chem. 37 1177-1184.
-
(1973)
Agric. Biol. Chem.
, vol.37
, pp. 1177-1184
-
-
Oka, T.1
Ishino, K.2
Tsuzuki, H.3
Morihara, K.4
Arima, K.5
-
17
-
-
0014724907
-
The isolation and partial characterization of an acid protease produced by Mucor miehei
-
M. Ottesen and W. Rickert (1970) The isolation and partial characterization of an acid protease produced by Mucor miehei. C. R. Trav. Lab. Carlsberg 37 301-325.
-
(1970)
C. R. Trav. Lab. Carlsberg
, vol.37
, pp. 301-325
-
-
Ottesen, M.1
Rickert, W.2
-
18
-
-
0029862567
-
Involvement of a residue at position 75 in the catalytic mechanism of a fungal aspartic proteinase, Rhizomucor pusillus pepsin. Replacement of tyrosine 75 on the flap by asparagine enhances catalytic efficiency
-
Y.-N. Park, J. Aikawa, M. Nishiyama, S. Horinouchi and T. Beppu (1996) Involvement of a residue at position 75 in the catalytic mechanism of a fungal aspartic proteinase, Rhizomucor pusillus pepsin. Replacement of tyrosine 75 on the flap by asparagine enhances catalytic efficiency. Protein Eng. 9 869-875.
-
(1996)
Protein Eng.
, vol.9
, pp. 869-875
-
-
Park, Y.-N.1
Aikawa, J.2
Nishiyama, M.3
Horinouchi, S.4
Beppu, T.5
-
19
-
-
0031024081
-
Site-directed mutagenesis of conserved Trp39 in Rhizomucor pusillus pepsin: possible role of Trp39 in maintaining Tyr75 in the correct orientation for maximizing catalytic activity
-
Y.-N. Park, J. Aikawa, M. Nishiyama, S. Horinouchi and T. Beppu (1997) Site-directed mutagenesis of conserved Trp39 in Rhizomucor pusillus pepsin: possible role of Trp39 in maintaining Tyr75 in the correct orientation for maximizing catalytic activity. J. Biochem. 121 118-121.
-
(1997)
J. Biochem.
, vol.121
, pp. 118-121
-
-
Park, Y.-N.1
Aikawa, J.2
Nishiyama, M.3
Horinouchi, S.4
Beppu, T.5
-
21
-
-
0014902977
-
The degradation of the B-chain of oxidized insulin by Mucor miehei protease
-
W. Rickert (1970) The degradation of the B-chain of oxidized insulin by Mucor miehei protease. C. R. Trav. Lab. Carlsberg 38 1-17.
-
(1970)
C. R. Trav. Lab. Carlsberg
, vol.38
, pp. 1-17
-
-
Rickert, W.1
-
22
-
-
0001779042
-
On the genera Rhizomucor Parasitella.
-
Inst. Roy. Netherlands Acad. Sci. Lett., 53–71.
-
Schipper, M.A.A. (1978) On the genera Rhizomucor Parasitella. Studies in Mycology. No. 17. Inst. Roy. Netherlands Acad. Sci. Lett. 53–71.
-
(1978)
Studies in Mycology.
, Issue.17
-
-
Schipper, M.A.A.1
-
23
-
-
0000567841
-
Studies on the optimum pH for the action of pepsin on native and denatured bovine serum albumin and bovine haemoglobin
-
M. Schlamowitz and L.U. Peterson (1959) Studies on the optimum pH for the action of pepsin on native and denatured bovine serum albumin and bovine haemoglobin. J. Biol. Chem. 234 3137-3145.
-
(1959)
J. Biol. Chem.
, vol.234
, pp. 3137-3145
-
-
Schlamowitz, M.1
Peterson, L.U.2
-
24
-
-
0014273840
-
Purification and properties of Mucor pusillus acid protease
-
G.A. Somkuti and F.J. Babel (1968) Purification and properties of Mucor pusillus acid protease. J. Bacteriol. 95 1407-1414.
-
(1968)
J. Bacteriol.
, vol.95
, pp. 1407-1414
-
-
Somkuti, G.A.1
Babel, F.J.2
-
25
-
-
0001025991
-
Crystalline milk-clotting protease from Mucor miehei and some of its properties
-
M.Z. Sternberg (1971) Crystalline milk-clotting protease from Mucor miehei and some of its properties. J. Dairy Sci. 54 150-167.
-
(1971)
J. Dairy Sci.
, vol.54
, pp. 150-167
-
-
Sternberg, M.Z.1
-
26
-
-
0015527839
-
Bond specificity, active site and milk clotting mechanism of the Mucor miehei protease
-
M. Sternberg (1972) Bond specificity, active site and milk clotting mechanism of the Mucor miehei protease. Biochim. Biophys. Acta 285 383-392.
-
(1972)
Biochim. Biophys. Acta
, vol.285
, pp. 383-392
-
-
Sternberg, M.1
-
27
-
-
0017194908
-
The structure and function of acid proteases. IV. Inactivation of the acid protease from Mucor pusillus by acid protease-specific inhibitors
-
K. Takahashi, W.J. Chang and K. Arima (1976) The structure and function of acid proteases. IV. Inactivation of the acid protease from Mucor pusillus by acid protease-specific inhibitors. J. Biochem. (Tokyo) 80 61-67.
-
(1976)
J. Biochem. (Tokyo)
, vol.80
, pp. 61-67
-
-
Takahashi, K.1
Chang, W.J.2
Arima, K.3
-
28
-
-
0023046310
-
Cloning and sequencing of a gene for Mucor rennin, an aspartate protease from Mucor pusillus
-
N. Tonouchi, H. Shoun, T. Uozumi and T. Beppu (1986) Cloning and sequencing of a gene for Mucor rennin, an aspartate protease from Mucor pusillus. Nucleic Acids Res. 14 7557-7568.
-
(1986)
Nucleic Acids Res.
, vol.14
, pp. 7557-7568
-
-
Tonouchi, N.1
Shoun, H.2
Uozumi, T.3
Beppu, T.4
-
29
-
-
0023478099
-
Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells
-
T. Yamashita, N. Tonouchi, T. Uozumi and T. Beppu (1987) Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells. Mol. Gen. Genet. 210 462-467.
-
(1987)
Mol. Gen. Genet.
, vol.210
, pp. 462-467
-
-
Yamashita, T.1
Tonouchi, N.2
Uozumi, T.3
Beppu, T.4
-
30
-
-
0033104868
-
Structure of the Rhizomucor miehei aspartic proteinase complexed with the inhibitor pepstatin A at 2.7 Å resolution
-
J. Yang and J.W. Quail (1999) Structure of the Rhizomucor miehei aspartic proteinase complexed with the inhibitor pepstatin A at 2.7 Å resolution. Acta Crystallogr. D Biol. Crystallogr. 55 625-630.
-
(1999)
Acta Crystallogr. D Biol. Crystallogr.
, vol.55
, pp. 625-630
-
-
Yang, J.1
Quail, J.W.2
-
31
-
-
17144458475
-
Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 Å resolution
-
J. Yang, A. Teplyakov and J.W. Quail (1997) Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 Å resolution. J. Mol. Biol. 268 449-459.
-
(1997)
J. Mol. Biol.
, vol.268
, pp. 449-459
-
-
Yang, J.1
Teplyakov, A.2
Quail, J.W.3
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