Site-directed mutagenesis of conserved Trp39 in Rhizomucor pusillus pepsin: Possible role of Trp39 in maintaining Tyr75 in the correct orientation for maximizing catalytic activity

Journal of Biochemistry

Volumn 121, Issue 1, 1997, Pages 118-121

  Park, Young Nam ^a,c   Aikawa, Jun Ichi ^a,d   Nishiyama, Makoto ^a   Horinouchi, Sueharu ^a   Beppu, Teruhiko ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b NIHON UNIVERSITY   (Japan)

^c BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^d INSTITUTE FOR PHYSICAL RESEARCH   (Armenia)

Author keywords

Aspartic proteinase; Milk clotting enzyme; Rhizomucor pusillus pepsin; Site directed mutagenesis

Indexed keywords

ASPARTIC PROTEINASE; CHROMOGENIC SUBSTRATE; FUNGAL PROTEIN; MUTANT PROTEIN; PEPSIN A; TRYPTOPHAN; TYROSINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; MILK; MUCORALES; NONHUMAN; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY;

FELIS CATUS; MUCORALES; RHIZOMUCOR PUSILLUS;

EID: 0031024081     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021553     Document Type: Article

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