Nuclear heterogeneity in conidial populations of Aspergillus flavus

Fungal Genetics and Biology

Volumn 84, Issue , 2015, Pages 62-72

  Runa, Farhana ^a   Carbone, Ignazio ^a   Bhatnagar, Deepak ^b   Payne, Gary A ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

A. flavus; Diploid; Flow cytometry; Fluorescence microscopy; Heterokaryon; Heterokaryosis; Ploidy

Indexed keywords

CYAN FLUORESCENT PROTEIN; HISTONE H2A; HISTONE H2B; YELLOW FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NUCLEOSOME; PHOTOPROTEIN;

ARTICLE; ASPERGILLUS FLAVUS; CARBOXY TERMINAL SEQUENCE; CONIDIUM; CONTROLLED STUDY; DIPLOIDY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FUNGAL STRAIN; FUNGUS CULTURE; GENETIC HETEROGENEITY; NONHUMAN; NUTRITIONAL REQUIREMENT; PLASMID; PRIORITY JOURNAL; REPORTER GENE; CELL FUSION; CHEMISTRY; FUNGUS SPORE; GENETIC VARIATION; GENETICS; GENOTYPE; METABOLISM; NUCLEOSOME; PLOIDY; PROCEDURES;

ASPERGILLUS FLAVUS; CELL FUSION; DIPLOIDY; FLOW CYTOMETRY; GENETIC VARIATION; GENOTYPE; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; NUCLEOSOMES; PLOIDIES; RECOMBINANT FUSION PROTEINS; SPORES, FUNGAL;

EID: 84943554765     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2015.09.003     Document Type: Article

References (44)

1. Atehnkeng J., Ojiambo P.S., Donner M., Ikotun T., Sikor R.A., Cotty P.J., Bandyopadhyay R. Distribution and toxigenicity of Aspergillus species isolated from maize kernels from three agroecological zones in Nigeria. Int. J. Food Microbiol. 2008, 122:74-84.
2. Bayman P., Cotty P.J. Genetic diversity in Aspergillus flavus: association with aflatoxin production and morphology. Can. J. Bot. 1993, 71:23-34.
3. Chang P., Horn B.W., Dorner J.W. Sequence break-points in the aflatoxin biosynthesis gene cluster and flanking regions in non-aflatoxigenic Aspergillus flavus isolates. Fungal Genet. Biol. 2005, 42:914-923.
4. Cotty P.J., Jaime-Garcia R. Influences of climate on aflatoxin producing fungi and aflatoxin contamination. Int. J. Food Microbiol. 2007, 119:109-115.
5. Ehrlich K.C., Montalbano B.G., Cotty P.J. Analysis of single nucleotide polymorphisms in three genes shows evidence for genetic isolation of certain Aspergillus flavus vegetative compatibility groups. FEMS Microbiol. Lett. 2007, 268:231-236.
6. EPA Aspergillus flavus, 2003. July 3, 2003 Biopesticide Registration Action Document. Available from <>. http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006456.pdf.
7. Foundin L.L., Papa K.E., Hanlin R.T. Nuclear behavior during conidiogenesis in Aspergillus flavus. Can. J. Bot. 1981, 59:2116-2120.
8. Geiser D.M., Pitt J.I., Taylor J.W. Cryptic speciation and recombination in the aflatoxin-producing fungus Aspergillus flavus. Proc. Natl. Acad. Sci. USA 1998, 95:388-393.
9. Georgianna D.R., Fedorova N.D., Burroughs J.L., Dolezal A.L., Bok J.W., Horowitz-Brown S., Woloshuk C.P., Yu J., Keller N.P., Payne G.A. Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters. Mol. Plant Pathol. 2010, 11(2):213-226.
10. Grubisha L.C., Cotty P.J. Twenty-four microsatellite markers for the aflatoxin-producing fungus Aspergillus flavus. Mol. Ecol. Resour. 2009, 9:264-267.
11. Grubisha L.C., Cotty P.J. Genetic isolation among sympatric vegetative compatibility groups of the aflatoxin-producing fungus Aspergillus flavus. Mol. Ecol. 2010, 19:269-280.
12. Hara S., Tsuji R.F., Hatamoto O., Masuda T. A simple method for enrichment of uninucleate conidia of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 2002, 66(3):693-696.
13. Hara S., Jin F.J., Takahashi T., Koyama Y. A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae. Mol. Genet. Genomics. 2012, 287(2):177-187. 10.1007/s00438-011-0669-1.
14. He Z.M., Price M.S., O'Brian G.R., Georgianna D.R., Payne G.A. Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus. BMC Microbiol. 2007, 7:104. 10.1186/1471-2180-7-104.
15. Hedayati M.T., Pasqualotto A.C., Warn P.A., Bowyer P., Denning D.W. Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 2007, 153:1677-1692.
16. Horn B.W., Greene R.L. Vegetative compatibility within populations of Aspergillus flavus, A. parasiticus, and A. tamarri from a peanut field. Mycologia 1995, 87:324-332.
17. Horn B.W., Greene R.L., Sobolev V.S. Association of morphology and mycotoxin production with vegetative compatibility groups in Aspergillus flavus, A. parasiticus, and A. tamarii. Mycologia 1996, 88:574-587.
18. Horn B.W., Moore G.G., Carbone I. Sexual reproduction in Aspergillus flavus. Mycologia 2009, 101:423-429.
19. Horn B.W., Sorensen R.B., Lamb M.C., Sobolev V.S., Olarte R.A., Worthington C.J., Carbone I. Sexual reproduction in Aspergillus flavus sclerotia naturally produced in corn. Phytopathology 2013, 104:75-85.
20. Ishi K., Maruyama J., Juvvadi P.R., NakaJima H., KitaMoto K. Nuclear migration during conidiophore development in Aspergillus nidulans and Aspergillus oryzae: multinucleation of conidia occurs through direct migration of plural nuclei from phialides and confers greater viability and early Germination in Aspergillus oryzae. Biosci. Biotechnol. Biochem. 2005, 69(4):747-754.
21. Jaime-Garcia R., Cotty P.J. Spatial distribution of Aspergillus flavus and its toxigenic strains on commercial cottonseed from south Texas and its relationship to aflatoxin contamination. Plant Pathol. 2006, 55:358-366.
22. James T.Y., Stenlid J., Olson A., Johannesson H. Evolutionary significance of imbalanced nuclear ratios within heterokaryons of the basidiomycete fungus Heterobasidion parviporum. Evolution 2008, 62(9):2279-2296. 10.1111/j.1558-5646.2008.00462.x.
23. Keller N.P., Cleveland T.E., Bhatnagar D. Variable electrophoretic karyotypes of members of Aspergillus Section Flavi. Curr. Genet. 1992, 21:371-375.
24. Klich M.A. Aspergillus flavus: the major producer of aflatoxin. Mol. Plant Pathol. 2007, 8:713-722.
25. Leslie J.F. Fungal vegetative compatibility. Annu. Rev. Phytopathol. 1993, 31:127-150.
26. Leaich L.L., Papa K.E. Identification of diploid of Aspergillus flavus by the nuclear condition. Mycologia 1975, 67:674-678.
27. Morpurgo G., Bellincampi D., Gaulandi G., Baldinelli L., Crescenzi O.S. Analysis of mitotic nondisjunction with Aspergillus nidulans. Environ. Health Perspect. 1979, 31:81-95.
28. Moore G.G., Singh R., Horn B.W., Carbone I. Recombination and lineage-specific gene loss in the aflatoxin gene cluster of Aspergillus flavus. Mol. Ecol. 2009, 18:4870-4887.
29. Moore G.G., Elliott J.L., Singh R., Horn B.W., Dorner J.W., Stone E.A., Chulze S.N., Barros G.G., Naik M.K., Wright G.C., Hell K., Carbone I. Sexuality generates diversity in the aflatoxin gene cluster: evidence on a global scale. PLoS Pathog. 2013, 9(8):e1003574. 10.1371/journal.ppat.1003574.
30. Maruyama J., Nakajima J., Kitamoto K. Visualization of nuclei in Aspergillus oryzae with EGFP and analysis of the number of nuclei in each conidium by FACS. Biosci. Biotechnol. Biochem. 2001, 65(7):1504-1510.
31. Nichols C.B., Fraser J.A., Heitman J. PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans. Mol. Biol. Cell. 2004, 15:4476-4489.
32. Olarte R.A., Horn B.W., Dorner J.W., Monacell J.T., Singh R., Stone E.A., Carbone I. Effect of sexual recombination on population diversity in aflatoxin production by Aspergillus flavus and evidence for cryptic heterokaryosis. Mol. Ecol. 2012, 21:1453-1476.
33. Papa K.E. The parasexual cycle in Aspergillus flavus. Mycologia 1973, 65:1201-1205.
34. Payne G.A., Nierman W.C., Wortman J.R., Pritchard B.L., Brown D., Dean R.A., Bhatnagar D., Cleveland T.E., Machida M., Yu J. Whole genome comparison of Aspergillus flavus and A. oryzae. Med. Mycol. 2006, 44:S9-S11.
35. Payne G.A., Yu J., Nierman W.C., Machida M., Bhatnagar D., Cleveland T.E., Dean R.A., Goldman G.H., Osmani S.A. A first glance into the genome sequence of Aspergillus flavus. The Aspergilli: Genomics, Medical Aspects, Biotechnology, and Research Method 2008, 15-23. CRC Press, Boca Raton, Florida. S.A. Osmani, G.H. Goldman (Eds.).
36. Pitchaimani K., Maheshwari R. Extreme nuclear disproportion and constancy of enzyme activity in a heterokaryon of Neurospora crassa. J. Genet. 2003, 82(1-2):1-6.
37. Probst C., Schulthess F., Cotty P.J. Impact of Aspergillus section Flavi community structure on the development of lethal levels of aflatoxins in Kenyan maize (Zeamays). J. Appl. Microbiol. 2010, 108:600-610.
38. Ramirez-Prado J.H., Moore G.G., Horn B.W., Carbone I. Characterization and population analysis of the mating- type genes in Aspergillus flavus and Aspergillus para-siticus. Fungal Genet. Biol. 2008, 45:1292-1299. 10.1016/j.fgb.2008.06.007.
39. Ramsdale M., Rayner A.D.M. Imbalanced nuclear ratios, post-germination mortality and phenotype-genotype relationships in allopatrically derived heterokaryons of Heterobasidion annosum. New Phytol. 1993, 133:303-319. 10.1111/j.1469-8137.1996.tb01898.x.
40. Rech C., Engh I., Kück U. Detection of hyphal fusion in filamentous fungi using differently fluorescence-labeled histones. Curr. Genet. 2007, 52:259-266.
41. Schroeder H.W., Boller R.A. Aflatoxin production of species and strains of the Aspergillus flavus group isolated from field crops. Appl. Microbiol. 1973, 25:885-889.
42. Wang D.Y., et al. Simple and Highly Discriminatory VNTR-Based Multiplex PCR for Tracing Sources of Aspergillus flavus Isolates. PLoS ONE 2012, 7(9):e44204.
43. Woloshuk C.P., Yousibova G.L., Rollins J.A., Bhatnagar D., Payne G.A. Molecular characterization of the Afl-1 locus in Aspergillus flavus. Appl. Environ. Microbiol. 1995, 61:3019-3023.
44. Yuill E. The numbers of nuclei in conidia of Aspergilli. Trans. Br. Mycol. Soc. 1950, 33:324-331.