Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR

Methods in Molecular Biology

Volumn 1339, Issue , 2015, Pages 309-322

  Rivera Torres, José ^a  

^a NONE

Author keywords

CDNA; Protein; Real time PCR; Reverse transcription; RNA; SYBR Green; Western blot

Indexed keywords

MESSENGER RNA; GENETIC MARKER;

ANIMAL TISSUE; ATHEROSCLEROSIS; GENE EXPRESSION; GENETIC ANALYSIS; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA ISOLATION; WESTERN BLOTTING; ANIMAL; AORTA; AORTA DISEASE; DISEASE MODEL; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; METABOLISM; PROCEDURES; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TIME FACTOR;

ANIMALS; AORTA; AORTIC DISEASES; ATHEROSCLEROSIS; BLOTTING, WESTERN; DISEASE MODELS, ANIMAL; GENE EXPRESSION REGULATION; GENETIC MARKERS; MICE; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TIME FACTORS;

EID: 84943523423     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2929-0_21     Document Type: Chapter

References (11)

1. Libby P, Ridker PM, Maseri A (2002) Inflammation and atherosclerosis. Circulation 105:1135-1143
2. Tomiyama H, Okazaki R, Koji Y et al (2005) Elevated C-reactive protein: a common marker for atherosclerotic cardiovascular risk and subclinical stages of pulmonary dysfunction and osteopenia in a healthy population. Atherosclerosis 178:187-192
3. Jamkhandea PG, Chandaka PG, Shashikant C et al (2013) Therapeutic approaches to drug targets in atherosclerosis. Saudi Pharm J. doi: 10.1016/j.jsps.2013.04.005
4. Wu SC, Zhang Y (2010) Active DNA demethylation: many roads lead to Rome. Nat Rev Mol Cell Biol 11:607-620
5. Stahlberg A, Bengtsson M (2010) Single-cell gene expression profiling using reverse transcription quantitative real-time PCR. Methods 50:282-288
6. Arya M, Shergill IS, Williamson M et al (2005) Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn 5:209-219
7. Getz GS, Reardon CA (2012) Animal models of atherosclerosis. Arterioscler Thromb Vasc Biol 32:1104-1115
8. Vasquez EC, Peotta VA, Gava AL et al (2012) Cardiac and vascular phenotypes in the apolipoprotein E-deficient mouse. J Biomed Sci 19:22
9. Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622
10. Hellemans J, Mortier G, De Paepe A et al (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
11. Nolan T, Hands RE, Bustin SA (2006) Quantification of mRNA using real-time RT-PCR. Nat Protoc 1:1559-1582