Development and validation of a SYBR Green real-time PCR assay for rapid and quantitative detection of goose interferons and proinflammatory cytokines

Poultry Science

Volumn 94, Issue 10, 2015, Pages 2382-2387

  Zhou, Hao ^a   Chen, Shun ^a   Qi, Yulin ^a   Wang, Mingshu ^a   Jia, Renyong ^a   Zhu, Dekang ^a   Liu, Mafeng ^a   Liu, Fei ^a   Chen, Xiaoyue ^a   Cheng, Anchun ^a  

^a SICHUAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cytokines; Goose; Real time quantitative PCR

Indexed keywords

AMINOQUINOLINE DERIVATIVE; AVIAN PROTEIN; IMIQUIMOD; INTERFERON; INTERFERON INDUCING AGENT; INTERLEUKIN DERIVATIVE; ORGANIC COMPOUND; SYBR GREEN I;

ANIMAL; DRUG EFFECTS; GENE EXPRESSION; GENETICS; GOOSE; MONONUCLEAR CELL; REAL TIME POLYMERASE CHAIN REACTION; SPLEEN; VALIDATION STUDY; VETERINARY;

AMINOQUINOLINES; ANIMALS; AVIAN PROTEINS; GEESE; GENE EXPRESSION; INTERFERON INDUCERS; INTERFERONS; INTERLEUKINS; LEUKOCYTES, MONONUCLEAR; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; SPLEEN;

EID: 84943402897     PISSN: 00325791     EISSN: 15253171     Source Type: Journal    
DOI: 10.3382/ps/pev241     Document Type: Article

References (20)

1. Bilu, D., and D. N. Sauder. 2003. Imiquimod: modes of action. Br. J. Dermatol. 149 Suppl 66:5-8.
2. Bolha, L., D. Bencina, I. Cizelj, I. Oven, B. Slavec, O. Z. Rojs, and M. Narat. 2013. Effect of Mycoplasma synoviae and lentogenic Newcastle disease virus coinfection on cytokine and chemokine gene expression in chicken embryos. Poult. Sci. 92: 3134-3143.
3. Cornelissen, J. B., J. Post, B. Peeters, L. Vervelde, and J. M. Rebel. 2012. Differential innate responses of chickens and ducks to lowpathogenic avian influenza. Avian. Pathol. 41:519-529.
4. Dinarello, C. A. 1999. IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J. Allergy. Clin. Immunol. 103:11-24.
5. Dinarello, C. A. 2011. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 117:3720-3732.
6. Gadde, U., H. D. Chapman, T. Rathinam, and G. F. Erf. 2011. Cellular immune responses, chemokine, and cytokine profiles in Turkey poults following infection with the intestinal parasite Eimeria adenoeides. Poult. Sci. 90:2243-2250.
7. Gibson, U. E., C. A. Heid, and P. M. Williams. 1996. A novel method for real time quantitative RT-PCR. Genome. Res. 6:995-1001.
8. Gu, C. Q., C. Q. Xie, X. Y. Hu, W. P. Zhang, D. R. Bi, and G. F. Cheng. 2012. Cytokine gene expression in the livers of ducklings infected with duck hepatitis virus-1 JX strain. Poult. Sci. 91:583-591.
9. Heid, C. A., J. Stevens, K. J. Livak, and P. M. Williams. 1996. Real time quantitative PCR. Genome. Res. 6:986-994.
10. Hirano, T. 1998. Interleukin 6 and its receptor: ten years later. Int. Rev. Immunol. 16:249-284.
11. Kim, K., H. Katayama, M. Kitajima, Y. Tohya, and S. Ohgaki. 2011. Development of a real-time RT-PCR assay combined with ethidium monoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure. Water. Sci. Technol. 63:502-507.
12. Li, Z., L. Cui, M. Zhou, X. Qi, C. Bao, J. Hu, J. Shan, B. Wu, S. Wang, X. Guo, Y. Jiao, F. Tang, and H. Wang. 2013. Development and application of a one-step real-time RT-PCR using a minor-groove-binding probe for the detection of a novel bunyavirus in clinical specimens. J. Med. Virol. 85:370-377.
13. Mackay, I. M., K. E. Arden, and A. Nitsche. 2002. Real-time PCR in virology. Nucleic. Acids. Res. 30:1292-1305.
14. Okamura, H., H. Tsutsi, T. Komatsu, M. Yutsudo, A. Hakura, T. Tanimoto, K. Torigoe, T. Okura, Y. Nukada, K. Hattori, K. Akita, M. Namba, F. Tanabe, K. Konishi, S. Fukuda, and M. Kurimoto. 1995. Cloning of a new cytokine that induces IFNgamma production by T cells. Nature. 378:88-91.
15. Schmittgen, T. D. 2001. Real-time quantitative PCR. Methods. 25:383-385.
16. Schoenborn, J. R., and C. B. Wilson. 2007. Regulation of interferongamma during innate and adaptive immune responses. Adv. Immunol. 96:41-101.
17. Schroder, K., P. J. Hertzog, T. Ravasi, and D. A. Hume. 2004. Interferon-gamma: an overview of signals, mechanisms and functions. J. Leukoc. Biol. 75:163-189.
18. Stoll, S., G. Muller, M. Kurimoto, J. Saloga, T. Tanimoto, H. Yamauchi, H. Okamura, J. Knop, and A. H. Enk. 1997. Production of IL-18 (IFN-gamma-inducing factor) messenger RNA and functional protein by murine keratinocytes. J. Immunol. 159:298-302.
19. Uno, Y., T. Usui, Y. Fujimoto, T. Ito, and T. Yamaguchi. 2012. Quantification of interferon, interleukin, and Toll-like receptor 7 mRNA in quail splenocytes using real-time PCR. Poult. Sci. 91:2496-2501.
20. Zhao, Q., S. Chen, F. Liu, Y. Qi, M. Wang, R. Jia, D. Zhu, M. Liu, and A. Cheng. 2014. Development and Validation of an Effi cient Real-time Quantitative Reverse Transcription Polymerase Chain Reaction Assay of Chinese Goose CD4 and CD8?. Acta. Sci. Vet. 42:1-5.