Single-cell analysis of T-cell receptor αβ repertoire

Methods in Molecular Biology

Volumn 1343, Issue , 2015, Pages 181-197

  Dash, Pradyot ^a   Wang, George C ^a   Thomas, Paul G ^a  

^a NONE

Author keywords

Clonotype; Complementarity determining region 3; Epitope; Paired analysis; Single cell analysis; T cell receptor repertoire; TCR alpha; TCR beta; TCR diversity; Unbiased repertoire

Indexed keywords

EPITOPE; T LYMPHOCYTE RECEPTOR; T LYMPHOCYTE RECEPTOR ALPHA CHAIN; LYMPHOCYTE ANTIGEN RECEPTOR;

COMPLEMENTARITY DETERMINING REGION; GEL ELECTROPHORESIS; GENE SEQUENCE; GENE TARGETING; HUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SINGLE CELL ANALYSIS; CHEMISTRY; FLOW CYTOMETRY; GENETICS; METABOLISM; PROCEDURES; T LYMPHOCYTE;

FLOW CYTOMETRY; HUMANS; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SINGLE-CELL ANALYSIS; T-LYMPHOCYTES;

EID: 84942858518     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2963-4_15     Document Type: Chapter

References (15)

1. Jorgensen JL, Esser U, de St F, Groth B, Reay PA, Davis MM (1992) Mapping T-cell receptor- peptide contacts by variant peptide immunization of single-chain transgenics. Nature 355:224–230
2. Davis MM, Bjorkman PJ (1988) T-cell antigen receptor genes and T-cell recognition. Nature 334:395–402
3. Blackwell TK, Alt FW (1989) Molecular characterization of the lymphoid V(D)J recombination activity. J Biol Chem 264:10327–10330
4. Valmori D, Dutoit V, Lienard D, Lejeune F, Speiser D, Rimoldi D, Cerundolo V, Dietrich PY, Cerottini JC, Romero P (2000) Tetramerguided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specifi c CD8+ T cells in melanoma patients. J Immunol 165:533–538
5. Pannetier C, Even J, Kourilsky P (1995) T-cell repertoire diversity and clonal expansions in normal and clinical samples. Immunol Today 16:176–181
6. Trautmann L, Rimbert M, Echasserieau K, Saulquin X, Neveu B, Dechanet J, Cerundolo V, Bonneville M (2005) Selection of T cell clones expressing high-affi nity public TCRs within Human cytomegalovirus-specifi c CD8 T cell responses. J Immunol 175:6123–6132
7. Argaet VP, Schmidt CW, Burrows SR, Silins SL, Kurilla MG, Doolan DL, Suhrbier A, Moss DJ, Kieff E, Sculley TB, Misko IS (1994) Dominant selection of an invariant T cell antigen receptor in response to persistent infection by Epstein- Barr virus. J Exp Med 180:2335–2340
8. Price DA, Brenchley JM, Ruff LE, Betts MR, Hill BJ, Roederer M, Koup RA, Migueles SA, Gostick E, Wooldridge L, Sewell AK, Connors M, Douek DC (2005) Avidity for antigen shapes clonal dominance in CD8+ T cell populations specifi c for persistent DNA viruses. J Exp Med 202:1349–1361
9. Wang C, Sanders CM, Yang Q, Schroeder HW Jr, Wang E, Babrzadeh F, Gharizadeh B, Myers RM, Hudson JR Jr, Davis RW, Han J (2010) High throughput sequencing reveals a complex pattern of dynamic interrelationships among human T cell subsets. Proc Natl Acad Sci U S A 107:1518–1523
10. Sherwood AM, Desmarais C, Livingston RJ, Andriesen J, Haussler M, Carlson CS, Robins H (2011) Deep sequencing of the human TCR? and TCRß repertoires suggests that TCRß rearranges after aß and ?d T Cell commitment. Sci Transl Med 3:90ra61
11. Dash P, McClaren JL, Oguin TH III, Rothwell W, Todd B, Morris MY, Becksfort J, Reynolds C, Brown SA, Doherty PC, Thomas PG (2011) Paired analysis of TCRa and TCRß chains at the single-cell level in mice. J Clin Invest 121:288–295
12. Wang GC, Dash P, McCullers JA, Doherty PC, Thomas PG (2012) T cell receptor aß diversity inversely correlates with pathogenspecifi c antibody levels in human cytomegalovirus infection. Sci Transl Med 4:128ra42
13. Sun X, Saito M, Sato Y, Chikata T, Naruto T, Ozawa T, Kobayashi E, Kishi H, Muraguchi A, Takiguchi M (2012) Unbiased analysis of TCRa/ß chains at the single-cell level in human CD8+ T-cell subsets. PLoS One 7:e40386
14. Ewert S, Huber T, Honegger A, Pluckthun A (2003) Biophysical properties of human antibody variable domains. J Mol Biol 325: 531–553
15. Lefranc MP, Giudicelli V, Ginestoux C, Jabado- Michaloud J, Folch G, Bellahcene F, Wu Y, Gemrot E, Brochet X, Lane J, Regnier L, Ehrenmann F, Lefranc G, Duroux P (2009) IMGT, the international Im Muno Gene Tics information system. Nucleic Acids Res 37:D1006–D1012