Determination of DNase activity by degradation of ethidium bromide–DNA complexes using a fluorescence plate reader

Analytical Biochemistry

Volumn 471, Issue , 2015, Pages 73-79

  Vogel, Benjamin ^a   Frantz, Stefan ^b  

^a UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

^b MARTIN LUTHER UNIVERSITÄT HALLE WITTENBERG   (Germany)

Author keywords

DNase activity; DNase1; Ethidium bromide fluorescence; Free chromatin

Indexed keywords

CHROMOSOMES; CLINICAL RESEARCH; LABORATORIES; PROTEINS;

ABSOLUTE QUANTIFICATION; BACKGROUND CORRECTION; DNASE ACTIVITY; DNASE1; ETHIDIUM BROMIDE; FREE CHROMATIN; LABORATORY EQUIPMENTS; QUANTITATIVE KINETICS;

FLUORESCENCE;

DEOXYRIBONUCLEASE; DNA; ETHIDIUM BROMIDE; ETHIDIUM;

ARTICLE; BLEACHING; CONTROLLED STUDY; DATA ANALYSIS; DEGRADATION; ENZYME ACTIVITY; FLUORESCENCE; INCUBATION TIME; KINETICS; LABORATORY DEVICE; MICROPLATE READER; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; CALIBRATION; CHEMISTRY; ENZYME ASSAY; METABOLISM; PROCEDURES; SPECTROFLUOROMETRY;

CALIBRATION; DEOXYRIBONUCLEASES; DNA; ENZYME ASSAYS; ETHIDIUM; KINETICS; SPECTROMETRY, FLUORESCENCE;

EID: 84942436729     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.11.013     Document Type: Article

References (25)

1. [1] Napirei, M., Ricken, A., Eulitz, D., Knoop, H., Mannherz, H.G., Expression pattern of the deoxyribonuclease 1 gene: lessons from the DNase1 knockout mouse. Biochem. J. 380 (2004), 929–937.
2. [2] Babina, S.E., Kanyshkova, T.G., Buneva, V.N., Nevinsky, G.A., Lactoferrin is the major deoxyribonuclease of human milk. Biochemistry 69 (2004), 1006–1015.
3. [3] Lu, S.-C., Shih, C.-H., Liao, T.-H., Expression of DNase I in rat parotid gland and small intestine is regulated by starvation and refeeding. J. Nutr. 133 (2003), 71–74.
4. [4] Oliveri, M., Daga, A., Cantoni, C., Lunardi, C., Millo, R., Puccetti, A., DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis. Eur. J. Immunol. 31 (2001), 743–751.
5. [5] Hirsch, J.G., Bactericidal action of histone. J. Exp. Med. 108 (1958), 925–944.
6. [6] Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D.S., Weinrauch, Y., Zychlinsky, A., Neutrophil extracellular traps kill bacteria. Science 303 (2004), 1532–1535.
7. [7] Papayannopoulos, V., Zychlinsky, A., NETs: a new strategy for using old weapons. Trends Immunol. 30 (2009), 513–521.
8. [8] Saffarzadeh, M., Juenemann, C., Queisser, M.A., Lochnit, G., Barreto, G., Galuska, S.P., Lohmeyer, J., Preissner, K.T., Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones. PLoS One, 7, 2012, e32366, 10.1371/journal.pone.0032366.
9. [9] Brill, A., Fuchs, T.A., Savchenko, A., Thomas, G.M., Martinod, K., De Meyer, S.F., Bhandari, A.A., Wagner, D.D., Neutrophil extracellular traps promote deep vein thrombosis in mice. J. Thromb. Haemost. 10 (2011), 136–144.
10. [10] Brinkmann, V., Zychlinsky, A., Neutrophil extracellular traps: is immunity the second function of chromatin?. J. Cell Biol. 198 (2012), 773–783.
11. [11] Papayannopoulos, V., Staab, D., Zychlinsky, A., Neutrophil elastase enhances sputum solubilization in cystic fibrosis patients receiving DNase therapy. PLoS ONE, 6, 2011, e28526, 10.1371/journal.pone.0028526.
12. [12] Buchanan, J.T., Simpson, A.J., Aziz, R.K., Liu, G.Y., Kristian, S.A., Kotb, M., Feramisco, J., Nizet, V., DNase expression allows the pathogen group A Streptococcus to escape killing in neutrophil extracellular traps. Curr. Biol. 16 (2006), 396–400.
13. [13] Kawai, Y., Yoshida, M., Arakawa, K., Kumamoto, T., Morikawa, N., Masamura, K., Tada, H., Ito, S., Hoshizaki, H., Oshima, S., et al. Diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in acute myocardial infarction. Circulation 109 (2004), 2398–2400.
14. [14] Sillesen, M., Jin, G., Oklu, R., Albadawi, H., Imam, A.M., Jepsen, C.H., Hwabejire, J.O., Ostrowski, S.R., Johansson, P.I., Rasmussen, L.S., et al. Fresh-frozen plasma resuscitation after traumatic brain injury and shock attenuates extracellular nucleosome levels and deoxyribonuclease 1 depletion. Surgery 154 (2013), 197–205.
15. [15] Spandidos, D.A., Ramandanis, G., Garas, J., Kottaridis, S.D., Serum deoxyribonucleases in patients with breast cancer. Eur. J. Cancer 16 (1980), 1615–1619.
16. [16] Kunitz, M., Crystalline desoxyribonuclease: isolation and general properties: spectrophotometric method for the measurement of desoxyribonuclease activity. J. Gen. Physiol. 33 (1950), 349–362.
17. [17] Choi, S.J., Szoka, F.C., Fluorometric determination of deoxyribonuclease I activity with PicoGreen. Anal. Biochem. 281 (2000), 95–97.
18. [18] Sinicropi, D., Baker, D.L., Prince, W.S., Shiffer, K., Shak, S., Colorimetric determination of DNase I activity with a DNA–methyl green substrate. Anal. Biochem. 222 (1994), 351–358.
19. [19] Fujihara, J., Tabuchi, M., Inoue, T., Yasuda, T., Fujita, Y., Takeshita, H., Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation. Anal. Biochem. 413 (2011), 78–79.
20. [20] Nakajima, T., Takagi, R., Tajima, Y., Makita, C., Kominato, Y., Kuribara, J., Ohshima, S., Tada, H., Tsurugaya, H., Kobayashi, Y., et al. Development of a sensitive enzyme-linked immunosorbent assay for measurement of DNase I in human serum. Clin. Chim. Acta Int. J. Clin. Chem. 403 (2009), 219–222.
21. [21] Cherepanova, A., Tamkovich, S., Pyshnyi, D., Kharkova, M., Vlassov, V., Laktionov, P., Immunochemical assay for deoxyribonuclease activity in body fluids. J. Immunol. Methods 325 (2007), 96–103.
22. [22] Nadano, D., Yasuda, T., Kishi, K., Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method. Clin. Chem. 39 (1993), 448–452.
23. [23] Friedhoff, P., Matzen, S.E., Meiss, G., Pingoud, A., A quantitative microtiter plate nuclease assay based on ethidium/DNA fluorescence. Anal. Biochem. 240 (1996), 283–288.
24. [24] Macanovic, M., Lachmann, P.J., Measurement of deoxyribonuclease I (DNase) in the serum and urine of systemic lupus erythematosus (SLE)-prone NZB/NZW mice by a new radial enzyme diffusion assay. Clin. Exp. Immunol. 108 (1997), 220–226.
25. [25] Yonemura, K., Maeda, H., A new assay method for DNase by fluorescence polarization and fluorescence intensity using DNA–ethidium bromide complex as a sensitive substrate. J. Biochem. 92 (1982), 1297–1303.