High-level expression of a recombinant active microbial transglutaminase in Escherichia coli

BMC Biotechnology

Volumn 15, Issue 1, 2015, Pages

  Salis, Barbara ^a   Spinetti, Gaia ^b   Scaramuzza, Silvia ^a   Bossi, Michele ^a   Saccani Jotti, Gloria ^c   Tonon, Giancarlo ^a   Crobu, Davide ^a   Schrepfer, Rodolfo ^a  

^a Bio Ker Srl Sardinia Scientific and Technological Park ^*  (Italy)

^b IRCCS MULTIMEDICA   (Italy)

^c UNIVERSITY OF PARMA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; ESCHERICHIA COLI; FOOD PROCESSING; HYDROGELS; PLANTS (BOTANY); PURIFICATION;

BIOCHEMICAL QUALITY; CLINICAL PERFORMANCE; CONSTITUTIVE EXPRESSIONS; ENZYMATIC ACTIVITIES; ESCHERICHIA COLI K-12; HIGH LEVEL EXPRESSION; MICROBIAL TRANSGLUTAMINASE; STREPTOMYCES MOBARAENSIS;

PROTEINS;

PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT PROTEIN; BACTERIAL PROTEIN;

ARTICLE; BACTERIAL STRAIN; BATCH PROCESS; CONTROLLED STUDY; ELECTROPORATION; ESCHERICHIA COLI; EXPRESSION VECTOR; HEAT SENSITIVITY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STREPTOMYCES; STREPTOMYCES MOBARAENSIS; CHEMISTRY; ENZYMOLOGY; FERMENTATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; PLASMID;

BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; FERMENTATION; PLASMIDS; RECOMBINANT PROTEINS; STREPTOMYCES; TRANSGLUTAMINASES;

EID: 84941564872     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/s12896-015-0202-4     Document Type: Article

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