Enhancement of ginsenoside Rg1 in Panax ginseng hairy root by overexpressing the α-l-rhamnosidase gene from Bifidobacterium breve

Biotechnology Letters

Volumn 37, Issue 10, 2015, Pages 2091-2096

  Zhang, Ru ^a   Zhang, Bian Ling ^a   Li, Gu Cai ^a   Xie, Tao ^a   Hu, Teng ^a   Luo, Zhi Yong ^b  

^a HUNAN INSTITUTE OF ENGINEERING   (China)

^b CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Bifidobacterium breve; Ginsenoside Rg1; Hairy roots; Panax ginseng; Transgenic hairy roots; l Rhamnosidase

Indexed keywords

ENZYME ACTIVITY; GENES;

BIFIDOBACTERIUM; GINSENOSIDE RG1; HAIRY ROOTS; PANAX GINSENG; RHAMNOSIDASE; TRANSGENIC HAIRY ROOTS;

PLANTS (BOTANY);

ALPHA-L-RHAMNOSIDASE; GINSENOSIDE; GINSENOSIDE RG 1; GLYCOSIDASE; RECOMBINANT PROTEIN;

AGROBACTERIUM; BIFIDOBACTERIUM; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PANAX; PLANT ROOT; TRANSGENIC PLANT;

AGROBACTERIUM; BIFIDOBACTERIUM; GENE EXPRESSION; GINSENOSIDES; GLYCOSIDE HYDROLASES; METABOLIC ENGINEERING; PANAX; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

EID: 84941415594     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-015-1889-y     Document Type: Article

References (17)

1. Bae EA, Shin JE, Kim DH (2005) Metabolism of ginsenoside Re by human intestinal microflora and its estrogenic effect. Biol Pharm Bull 28:1903–1908
2. De Winter K, Simcikova D, Schalck B, Weignerova L, Pelantova H, Soetaert W, Desmet T, Kren V (2013) Chemoenzymatic synthesis of alpha-l-rhamnosides using recombinant alpha-l-rhamnosidase from Aspergillus terreus. Bioresour Technol 147:640–644
3. Elujoba A, Hardman R (1987) Diosgenin production by acid and enzymatic hydrolysis of fenugreek. Fitoterapia 58:299–303
4. Gandhi SG, Mahajan V, Bedi YS (2015) Changing trends in biotechnology of secondary metabolism in medicinal and aromatic plants. Planta 241:303–317
5. Georgiev MI, Agostini E, Ludwig-Muller J, Xu J (2012) Genetically transformed roots: from plant disease to biotechnological resource. Trends Biotechnol 30:528–537
6. Hasegawa H, Lee KS, Nagaoka T, Tezuka Y, Uchiyama M, Kadota S, Saiki I (2000) Pharmacokinetics of ginsenoside deglycosylated by intestinal bacteria and its transformation to biologically active fatty acid esters. Biol Pharm Bull 23:298–304
7. Mehrotra S, Srivastava V, Rahman LU, Kukreja AK (2013) Overexpression of a Catharanthustryptophan decarboxylase (tdc) gene leads to enhanced terpenoid indole alkaloid (TIA) production in transgenic hairy root lines of Rauwolfia serpentina. Plant Cell Tiss Organ Cult 115:377–384
8. Murthy HN, Georgiev MI, Kim YS, Jeong CS, Kim SJ, Park SY, Paek KY (2014) Ginsenosides: prospective for sustainable biotechnological production. Appl Microbiol Biotechnol 98:6243–6254
9. Oh HJ, Shin KC, Oh DK (2014) Production of ginsenosides Rg1 and Rh1 by hydrolyzing the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides using beta-glucosidase from Pyrococcus furiosus. Biotechnol Lett 36:113–119
10. Runguphan W, Qu X, O’Connor SE (2010) Integrating carbon-halogen bond formation into medicinal plant metabolism. Nature 468:461–464
11. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3:1101–1108
12. Song Y, Zhao F, Zhang L, Du Y, Wang T, Fu F (2013) Ginsenoside Rg1 exerts synergistic anti-inflammatory effects with low doses of glucocorticoids in vitro. Fitoterapia 91:173–179
13. Thanh NT, Murthy HN, Yu KW, Hahn EJ, Paek KY (2005) Methyl jasmonate elicitation enhanced synthesis of ginsenoside by cell suspension cultures of Panax ginseng in 5-l balloon type bubble bioreactors. Appl Microbiol Biotechnol 67:197–201
14. Yeh E, Garneau S, Walsh CT (2005) Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis. Proc Natl Acad Sci U S A 102:3960–3965
15. Yu H, Gong J, Zhang C, Jin F (2002) Purification and characterization of ginsenoside-alpha-l-rhamnosidase. Chem Pharm Bull (Tokyo) 50:175–178
16. Zehner S, Kotzsch A, Bister B, Sussmuth RD, Mendez C, Salas JA, van Pee KH (2005) A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005. Chem Biol 12:445–452
17. Zhang R, Zhang B, Xie T, Li G, Yi T, Xiang Y (2015) Biotransformation of rutin to isoquercitrin using recombinant α-l-rhamnosidase from Bifidobacterium breve. Biotech Lett 37:1257–1264