qPCRTag analysis - A high throughput, real time PCR assay for Sc2.0 genotyping

Journal of Visualized Experiments

Volumn 2015, Issue 99, 2015, Pages

  Mitchell, Leslie A ^a   Phillips, Nick A ^a   Lafont, Andrea ^b   Martin, James A ^a   Cutting, Rupal ^b   Boeke, Jef D ^a  

^a Department of Biochemistry and Molecular Pharmacology Institute for Systems Genetics   (United States)

^b Roche Life Science ^*  (United States)

Author keywords

Cobra; Echo; Genotyping; Issue 99; LightCycler 1536; Molecular biology; PCRTag; Real time PCR; Saccharomyces cerevisiae; Sc2.0; Synthetic biology

Indexed keywords

FUNGAL DNA; PRIMER DNA;

CHEMISTRY; FUNGAL GENOME; GENETICS; GENOTYPING TECHNIQUE; HIGH THROUGHPUT SEQUENCING; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; YEAST ARTIFICIAL CHROMOSOME;

CHROMOSOMES, ARTIFICIAL, YEAST; DNA PRIMERS; DNA, FUNGAL; GENOME, FUNGAL; GENOTYPING TECHNIQUES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; REAL-TIME POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 84941243761     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52941     Document Type: Article

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