Analysis of Cell Metabolism Using LC-MS and Isotope Tracers

Methods in Enzymology

Volumn 561, Issue , 2015, Pages 171-196

  MacKay, Gillian M ^a   Zheng, Liang ^a   Van Den Broek, Niels J F ^a   Gottlieb, Eyal ^a  

^a BEATSON INSTITUTE FOR CANCER RESEARCH   (United Kingdom)

Author keywords

Exactive and Q Exactive mass spectrometry; Fumarate hydratase; HILIC chromatography; LC MS; Metabolite extraction; Metabolite quantification; Stable isotope tracers

Indexed keywords

CARBON 13; FUMARATE HYDRATASE; CARBON;

ADHERENT CELL; BLOOD SAMPLING; CARBON METABOLISM; CELL CULTURE; CELL GROWTH; CELL METABOLISM; CEREBROSPINAL FLUID; CULTURE MEDIUM; DATA ANALYSIS; EXTRACTION; HUMAN; HYDROPHILIC INTERACTION CHROMATOGRAPHY; ISOTOPE ANALYSIS; ISOTOPE TRACING; KINETICS; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW; SOFT TISSUE; SOLVENT EXTRACTION; TISSUES; URINALYSIS; ANIMAL; ISOTOPE LABELING; METABOLISM; PROCEDURES;

ANIMALS; CARBON; CARBON ISOTOPES; CHROMATOGRAPHY, LIQUID; HUMANS; ISOTOPE LABELING; MASS SPECTROMETRY;

EID: 84941025166     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.05.016     Document Type: Chapter

References (19)

1. B.D. Bennett, J. Yuan, E.H. Kimball, and J.D. Rabinowitz Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach Nature Protocols 3 8 2008 1299 1311
2. S.J. Bruce, I. Tavazzi, V. Parisod, S. Rezzi, S. Kochhar, and P.A. Guy Investigation of human blood plasma sample preparation for performing metabolomics using ultrahigh performance liquid chromatography/mass spectrometry Analytical Chemistry 81 9 2009 3285 3296
3. J.M. Buescher, M.R. Antoniewicz, L.G. Boros, S.C. Burgess, H. Brunengraber, C.B. Clish, and et al. A roadmap for interpreting C metabolite labeling patterns from cells Current Opinion in Biotechnology 34C 2015 189 201
4. J.M. Buescher, S. Moco, U. Sauer, and N. Zamboni Ultrahigh performance liquid chromatography-tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites Analytical Chemistry 82 11 2010 4403 4412
5. K. Dettmer, P.A. Aronov, and B.D. Hammock Mass spectrometry-based metabolomics Mass Spectrometry Reviews 26 1 2007 51 78
6. S. Dietmair, N.E. Timmins, P.P. Gray, L.K. Nielsen, and J.O. Kromer Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol Analytical Biochemistry 404 2 2010 155 164
7. O. Fiehn Metabolomics - The link between genotypes and phenotypes Plant Molecular Biology 48 1-2 2002 155 171
8. O. Fiehn, J. Kopka, R.N. Trethewey, and L. Willmitzer Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry Analytical Chemistry 72 15 2000 3573 3580
9. C. Frezza, L. Zheng, O. Folger, K.N. Rajagopalan, E.D. MacKenzie, L. Jerby, and et al. Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase Nature 477 7363 2011 225 228
10. A. King, M.A. Selak, and E. Gottlieb Succinate dehydrogenase and fumarate hydratase: Linking mitochondrial dysfunction and cancer Oncogene 25 34 2006 4675 4682
11. W. Lu, M.F. Clasquin, E. Melamud, D. Amador-Noguez, A.A. Caudy, and J.D. Rabinowitz Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer Analytical Chemistry 82 8 2010 3212 3221
12. B. Luo, K. Groenke, R. Takors, C. Wandrey, and M. Oldiges Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry Journal of Chromatography A 1147 2 2007 153 164
13. J.D. Rabinowitz, and E. Kimball Acidic acetonitrile for cellular metabolome extraction from Escherichia coli Analytical Chemistry 79 16 2007 6167 6173
14. C.A. Sellick, R. Hansen, G.M. Stephens, R. Goodacre, and A.J. Dickson Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling Nature Protocols 6 8 2011 1241 1249
15. K.L. Wade, I.J. Garrard, and J.W. Fahey Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates Journal of Chromatography A 1154 1-2 2007 469 472
16. O. Yanes, R. Tautenhahn, G.J. Patti, and G. Siuzdak Expanding coverage of the metabolome for global metabolite profiling Analytical Chemistry 83 6 2011 2152 2161
17. T. Zhang, D.J. Creek, M.P. Barrett, G. Blackburn, and D.G. Watson Evaluation of coupling reversed phase, aqueous normal phase, and hydrophilic interaction liquid chromatography with Orbitrap mass spectrometry for metabolomic studies of human urine Analytical Chemistry 84 4 2012 1994 2001
18. L. Zheng, S. Cardaci, L. Jerby, E.D. MacKenzie, M. Sciacovelli, T.I. Johnson, and et al. Fumarate induces redox-dependent senescence by modifying glutathione metabolism Nature Communications 6 2015 6001
19. L. Zheng, E.D. Mackenzie, S.A. Karim, A. Hedley, K. Blyth, G. Kalna, and et al. Reversed argininosuccinate lyase activity in fumarate hydratase-deficient cancer cells Cancer & Metabolism 1 1 2013 12