Probing effects of pressure release on virus capture during virus filtration using confocal microscopy

Biotechnology and Bioengineering

Volumn 112, Issue 10, 2015, Pages 2115-2122

  Dishari, Shudipto K ^a   Venkiteshwaran, Adith ^b,c   Zydney, Andrew L ^a  

^a The Center for Nanotechnology Education and Utilization   (United States)

^b ELI LILLY AND COMPANY   (United States)

^c HARVARD BUSINESS SCHOOL   (United States)

Author keywords

Bioprocessing; Confocal microscopy; Pressure release; Virus clearance; Virus filtration

Indexed keywords

CONFOCAL MICROSCOPY; FILTRATION; FLUORESCENCE; MICROFILTRATION; PORE STRUCTURE;

BIOPROCESSING; DIRECT VISUALIZATION; FILTRATION MEMBRANES; FLUORESCENCE TECHNIQUE; MEMBRANE MORPHOLOGY; PRESSURE RELEASE; RETENTION CHARACTERISTICS; VIRUS CLEARANCE;

VIRUSES;

CYANINE DYE 5; FLUORESCENT DYE; GREEN FLUORESCENT DYE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE; CONFOCAL LASER MICROSCOPY; CONFOCAL LASER SCANNING MICROSCOPE; ENTEROBACTERIA PHAGE P22; ESCHERICHIA COLI; FILTRATION; FLUORESCENCE; LIGHT SCATTERING; MEMBRANE FILTER; NONHUMAN; PRESSURE; PRESSURE REGULATOR; VIRAL CLEARANCE; VIRUS FILTRATION; VIRUS TRANSMISSION; BACTERIOPHAGE PHI X 174; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; HYDROSTATIC PRESSURE; ISOLATION AND PURIFICATION; MICROPORE FILTER; PROCEDURES; STAINING;

BACTERIOPHAGE PHI X 174; FILTRATION; FLUORESCENCE; HYDROSTATIC PRESSURE; MICROPORE FILTERS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; STAINING AND LABELING;

EID: 84940467080     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25614     Document Type: Article

References (12)

1. Bakhshayeshi M, Jackson N, Kuriyel R, Mehta A, van Reis R, Zydney AL. 2011. Use of confocal scanning laser microscopy to study virus retention during virus filtration. J Membrane Sci 379:260-267.
2. Bolton G, Cabatingan M, Rubino M, Lute S, Brorson K, Bailey M. 2005. Normal-flow virus filtration: Detection and assessment of the endpoint in bioprocessing. Biotechnol Appl Biochem 42:133-142.
3. Brough H, Antoniou C, Carter J, Jakubik J, Xu Y, Lutz H. 2002. Performance of a novel Viresolve NFR virus filter. Biotechnol Prog 18:782-795.
4. DiLeo AJ, Allegrezza AE, Builder SE. 1992. High resolution removal of virus from protein solutions using a membrane of unique structure. Nat Biotechnol 10:182-188.
5. DiLeo AJ, Vacante DA, Deane EF. 1993. Size exclusion removal of model mammalian viruses using a unique membrane system. Part II. Model qualification and process simulation. Biologicals 21:275-286.
6. Jackson NB, Bakhshayeshi M, Zydney AL, Mehta A, van Reis R, Kuriyel R. 2014. Internal virus polarization model for virus retention by the Ultipor® VF grade DV20 membrane. Biotechnol Prog 30:856-863.
7. Ireland T, Lutz H, Siwak M, Bolton G. 2004. Viral filtration of plasma-derived human IgG: A case study using Viresolve NFP. Biopharm Intl 17:38-44.
8. Lacasse D, Genest P, Pizzelli K, Greenhalgh P, Mullin L, Slocum A. 2013. Impact of process interruption on virus retention of small-virus filters. Bioprocess Intl 11:34-44.
9. Lute S. 2014. Mechanism of virus breakthrough during filter pausing. PDA/FDA Virus and TSE Safety Conference, Bethesda, MD.
10. Lute S, Bailey M, Combs J, Sukumar M, Brorson K. 2007. Phage passage after extended processing in small-virus-retentive filters. Biotechnol Appl Biochem 47:141-151.
11. Mosier-Boss PA, Lieberman SH, Andrews JM, Rohwer FL, Wegley LE, Breitbart M. 2003. Use of fluorescently labeled phage in the detection and identification of bacterial species. Appl Spec 57:1138-1144.
12. Woods MA, Zydney AL. 2014. Effects of a pressure release on virus retention with the Ultipor DV20 membrane. Biotechnol Bioeng 111:545-551.