Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Applied Biochemistry and Biotechnology

Volumn 177, Issue 2, 2015, Pages 511-527

  Zhao, Junqi ^a   Guo, Chao ^a   Tian, Chaoguang ^a   Ma, Yanhe ^a  

^a Tianjin Airport Economic Area   (China)

Author keywords

Myceliophthora thermophila; Pichia pastoris; Substrate specificity; Transglycosylation activity; Glucosidase

Indexed keywords

ENZYMES; FUNGI; GENE ENCODING; GLYCOSYLATION; SUBSTRATES; YEAST;

GLUCOSIDASE; MYCELIOPHTHORA THERMOPHILA; PICHIA PASTORIS; SUBSTRATE SPECIFICITY; TRANS GLYCOSYLATION ACTIVITY;

ENZYME ACTIVITY;

4 NITROPHENYL BETA DEXTRO GLUCOPYRANOSIDE; 4 NITROPHENYL DEXTRO CELLOBIOSIDE; BETA GLUCOSIDASE; CELLOBIOSE; CELLOTETRAOSE; CELLOTRIOSE; GENTIOBIOSE; GLUCOPYRANOSIDE; GLYCOSIDE HYDROLASE 3; UNCLASSIFIED DRUG; ION; METAL; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; HETEROLOGOUS EXPRESSION; KOMAGATAELLA PASTORIS; MOLECULAR DOCKING; MYCELIOPHTHORA THERMOPHILA; NONHUMAN; THERMOSTABILITY; CHEMISTRY; DNA SEQUENCE; DRUG EFFECTS; ENZYME SPECIFICITY; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION; GENETICS; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; SORDARIALES; STRUCTURAL HOMOLOGY;

CORYNASCUS HETEROTHALLICUS; FUNGI; PICHIA PASTORIS;

AMINO ACID SEQUENCE; BETA-GLUCOSIDASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENES, FUNGAL; GLYCOSYLATION; HYDROLYSIS; IONS; KINETICS; METALS; MOLECULAR DOCKING SIMULATION; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SORDARIALES; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY;

EID: 84940452964     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1759-z     Document Type: Article

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