The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt

International Journal of Medical Microbiology

Volumn 305, Issue 6, 2015, Pages 581-588

  Lopes, Bruno Silvester ^a   Al Agamy, Mohamed H ^b,c   Ismail, Muhammad A ^a   Shibl, Atef M ^b,d,e   Al Qahtani, Ahmed A ^d,e   Al Ahdal, Mohammed N ^d,e   Forbes, Ken J ^a  

^a Medical Microbiology   (United Kingdom)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

^c Cairo University   (Egypt)

^d KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTER   (Saudi Arabia)

^e ALFAISAL UNIVERSITY   (Saudi Arabia)

Author keywords

Acinetobacter baumannii; Carbapenems; Middle East; OXA 23; Plasmid

Indexed keywords

CEFTAZIDIME; CIPROFLOXACIN; IMIPENEM; BACTERIAL PROTEIN; CARBAPENEM DERIVATIVE;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; BLA ADC GENE; BLA NDM 1 GENE; BLA OXA 23 GENE; BLA OXA 24 GENE; BLA OXA 51 GENE; BLA VIM 2 GENE; EGYPT; GENE OVEREXPRESSION; HUMAN; HUMAN TISSUE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR EPIDEMIOLOGY; MULTIDRUG RESISTANCE; NONHUMAN; PLASMID; PULSED FIELD GEL ELECTROPHORESIS; SAUDI ARABIA; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL SENSITIVITY TEST; MOLECULAR GENETICS; MULTILOCUS SEQUENCE TYPING;

ACINETOBACTER BAUMANNII; BACTERIAL PROTEINS; CARBAPENEMS; CEFTAZIDIME; DRUG RESISTANCE, MULTIPLE, BACTERIAL; EGYPT; ELECTROPHORESIS, GEL, PULSED-FIELD; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; IMIPENEM; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MULTILOCUS SEQUENCE TYPING; PLASMIDS; SAUDI ARABIA;

EID: 84940450473     PISSN: 14384221     EISSN: 16180607     Source Type: Journal    
DOI: 10.1016/j.ijmm.2015.07.007     Document Type: Article

References (29)

1. Al-Agamy M.H., Khalaf N.G., Tawfick M.M., Shibl A.M., El Kholy A. Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt. Int. J. Infect. Dis. 2014, 22:49-54.
2. Al-Hassan L., El Mehallawy H., Amyes S.G. Diversity in Acinetobacter baumannii isolates from paediatric cancer patients in Egypt. Clin. Microbiol. Infect. 2013, 19:1082-1088.
3. Alsultan A.A., Evans B.A., Elsayed E.A., Al-Thawadi S.I., Al-Taher A.Y., Amyes S.G., Al-Dughaym A.M., Hamouda A. High frequency of carbapenem-resistant Acinetobacter baumannii in patients with diabetes mellitus in Saudi Arabia. J. Med. Microbiol. 2013, 62:885-888.
4. OXA-51-like genes in patients with diabetes mellitus. J. Chemother. 2009, 21:290-295.
5. Bartual S.G., Seifert H., Hippler C., Luzon M.A., Wisplinghoff H., Rodríguez-Valera F. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J. Clin. Microbiol. 2005, 43:4382-4390.
6. BSAC. Methods for Antimicrobial Susceptibility Testing-Version 14. Available from: (last accessed 09.04.15). http://bsac.org.uk/wp-content/uploads/2012/02/BSAC-Susceptibility-testing-version-14.pdf.
7. Castanheira M., Costello S.E., Woosley L.N., Deshpande L.M., Davies T.A., Jones R.N. Evaluation of clonality and carbapenem resistance mechanisms among Acinetobacter baumannii-Acinetobacter calcoaceticus complex and Enterobacteriaceae isolates collected in European and Mediterranean countries and detection of two novel β-lactamases, GES-22 and VIM-35. Antimicrob. Agents Chemother. 2014, 58:7358-7366.
8. Choi Y., Sims G.E., Murphy S., Miller J.R., Chan A.P. Predicting the functional effect of amino acid substitutions and indels. PLoS ONE 2012, 7:e46688.
9. Cicek A.C., Saral A., Iraz M., Ceylan A., Duzgun A.O., Peleg A.Y., Sandalli C. OXA- and GES-type β-lactamases predominate in extensively drug-resistant Acinetobacter baumannii isolates from a Turkish University Hospital. Clin. Microbiol. Infect. 2014, 20:410-415.
10. Corvec S., Caroff N., Espaze E., Giraudeau C., Drugeon H., Reynaud A. AmpC cephalosporinase hyperproduction in Acinetobacter baumannii clinical strains. J. Antimicrob. Chemother. 2003, 52:629-635.
11. OXA-23 in Acinetobacter baumannii. Antimicrob. Agents Chemother. 2007, 51:1530-1533.
12. del Mar Tomás M., Beceiro A., Pérez A., Velasco D., Moure R., Villanueva R., Martínez-Beltrán J., Bou G. Cloning and functional analysis of the gene encoding the 33- to 36-kilodalton outer membrane protein associated with carbapenem resistance in Acinetobacter baumannii. Antimicrob. Agents Chemother. 2005, 49:5172-5175.
13. Delbrück H., Bogaerts P., Kupper M.B., Rezende de Castro R., Bennink S., Glupczynski Y., Galleni M., Hoffmann K.M., Bebrone C. Kinetic and crystallographic studies of extended-spectrum GES-11, GES-12, and GES-14 β-lactamases. Antimicrob. Agents Chemother. 2012, 56:5618-5625.
14. Dijkshoorn L., Nemec A., Seifert H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 2007, 5:939-951.
15. Ellington M.J., Kistler J., Livermore D.M., Woodford N. Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J. Antimicrob. Chemother. 2007, 59:321-322.
16. KPC1/2-12 alleles from clinical samples by multiplex PCR-based assays. Int. J. Antimicrob. Agents 2013, 42:68-71.
17. Héritier C., Poirel L., Fournier P.E., Claverie J.M., Raoult D., Nordmann P. Characterization of the naturally occurring oxacillinase of Acinetobacter baumannii. Antimicrob. Agents Chemother. 2005, 49:4174-4179.
18. Higgins P.G., Pérez-Llarena F.J., Zander E., Fernández A., Bou G., Seifert H. OXA-235, a novel class D β-lactamase involved in resistance to carbapenems in Acinetobacter baumannii. Antimicrob. Agents Chemother. 2013, 57:2121-2126.
19. Hornsey M., Phee L., Wareham D.W. A novel variant, NDM-5, of the New Delhi metallo-β-lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom. Antimicrob. Agents Chemother. 2011, 55:5952-5954.
20. Karah N., Sundsfjord A., Towner K., Samuelsen O. Insights into the global molecular epidemiology of carbapenem non-susceptible clones of Acinetobacter baumannii. Drug Resist. Updat. 2012, 15:237-247.
21. La Scola B., Gundi V.A.K.B., Khamis A., Raoult D. Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species. J. Clin. Microbiol. 2006, 44:827-832.
22. OXA-58 gene in clinical isolates of Acinetobacter baumannii isolated from the USA. Clin. Microbiol. Infect. 2012, 18:E446-E451.
23. Lopes B.S., Gallego L., Amyes S.G. Multi-drug resistance profiles and the genetic features of Acinetobacter baumannii isolates from Bolivia. J. Infect. Dev. Ctries. 2013, 7:323-328.
24. Mugnier P.D., Bindayna K.M., Poirel L., Nordmann P. Diversity of plasmid-mediated carbapenem-hydrolysing oxacillinases among carbapenem-resistant Acinetobacter baumannii isolates from Kingdom of Bahrain. J. Antimicrob. Chemother. 2009, 63:1071-1073.
25. Mugnier P.D., Poirel L., Nordmann P. Functional analysis of insertion sequence ISAba1, responsible for genomic plasticity of Acinetobacter baumannii. J. Bacteriol. 2009, 191:2414-2418.
26. Mussi M.A., Limansky A.S., Viale A.M. Acquisition of resistance to carbapenems in multidrug-resistant clinical strains of Acinetobacter baumannii: natural insertional inactivation of a gene encoding a member of a novel family of beta-barrel outer membrane proteins. Antimicrob. Agents Chemother. 2005, 49:1432-1440.
27. Neela F.A., Nagahama N., Suzuki S. Cell-to-cell contact is required for transfer of tetracycline resistance gene tet(M) in marine bacteria. Interdisciplinary Studies on Environmental Chemistry-Biological Responses to Chemical Pollutants 2008, 349-353. TERRAPUB, Tokyo. Y. Murakami, K. Nakayama, S.-I. Kitamura, H. Iwata, S. Tanabe (Eds.).
28. Vila J., Martí S., Sánchez-Céspedes J. Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii. J. Antimicrob. Chemother. 2007, 59:1210-1215.
29. Zowawi H.M., Sartor A.L., Sidjabat H.E., Balkhy H.H., Walsh T.R., Al Johani S.M., AlJindan R.Y., Alfaresi M., Ibrahim E., Al-Jardani A., Al Salman J., Dashti A.A., Johani K., Paterson D.L. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolates in the Gulf Cooperation Council States: dominance of OXA-23-type producers. J. Clin. Microbiol. 2015, 53:576-903.