A multiplex real-time PCR assay for routine diagnosis of bacterial vaginosis

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 34, Issue 9, 2015, Pages 1779-1785

  Kusters, J G ^a   Reuland, E A ^b   Bouter, S ^c   Koenig, P ^c   Dorigo Zetsma, J W ^c  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^c TERGOOI HOSPITAL   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; ATOPOBIUM VAGINAE; BACTERIAL VAGINOSIS; CAUCASIAN; FEMALE; GARDNERELLA VAGINALIS; GYNECOLOGIST; HUMAN; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; MAJOR CLINICAL STUDY; MEGASPHAERA; MICROSCOPY; MULTIPLEX POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; QUESTIONNAIRE; REAL TIME POLYMERASE CHAIN REACTION; SELF REPORT; VAGINA DISCHARGE; VAGINA SMEAR; VAGINITIS; ADOLESCENT; GENETICS; MICROBIOLOGY; MIDDLE AGED; NETHERLANDS; PROCEDURES; PROSPECTIVE STUDY; VAGINA; VAGINAL DISCHARGE; VAGINOSIS, BACTERIAL; YOUNG ADULT;

BACTERIAL DNA; FUNGAL DNA;

ADOLESCENT; ADULT; DNA, BACTERIAL; DNA, FUNGAL; FEMALE; HUMANS; MIDDLE AGED; NETHERLANDS; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; SURVEYS AND QUESTIONNAIRES; VAGINA; VAGINAL DISCHARGE; VAGINAL SMEARS; VAGINOSIS, BACTERIAL; YOUNG ADULT;

EID: 84940439292     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-015-2412-z     Document Type: Article

References (23)

1. Dekker JH (2005) The Dutch Health Council report on screening for Chlamydia: too reserved. Ned Tijdschr Geneeskd 149(16):850–852
2. Myer L, Denny L, Telerant R, Souza M, Wright TC Jr, Kuhn L (2005) Bacterial vaginosis and susceptibility to HIV infection in South African women: a nested case–control study. J Infect Dis 192(8):1372–1380
3. Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK (1983) Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med 74(1):14–22
4. Nugent RP, Krohn MA, Hillier SL (1991) Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 29(2):297–301
5. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM (2007) Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J Clin Microbiol 45(10):3270–3276
6. Fredricks DN, Fiedler TL, Marrazzo JM (2005) Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 353(18):1899–1911
7. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D (2008) Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin Infect Dis 47(1):33–43
8. Menard JP, Mazouni C, Fenollar F, Raoult D, Boubli L, Bretelle F (2010) Diagnostic accuracy of quantitative real-time PCR assay versus clinical and Gram stain identification of bacterial vaginosis. Eur J Clin Microbiol Infect Dis 29(12):1547–1552
9. Zozaya-Hinchliffe M, Lillis R, Martin DH, Ferris MJ (2010) Quantitative PCR assessments of bacterial species in women with and without bacterial vaginosis. J Clin Microbiol 48(5):1812–1819
10. Cartwright CP, Lembke BD, Ramachandran K, Body BA, Nye MB, Rivers CA, Schwebke JR (2012) Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis. J Clin Microbiol 50(7):2321–2329
11. De Backer E, Verhelst R, Verstraelen H, Alqumber MA, Burton JP, Tagg JR, Temmerman M, Vaneechoutte M (2007) Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners. BMC Microbiol 7:115
12. Srinivasan S, Hoffman NG, Morgan MT, Matsen FA, Fiedler TL, Hall RW, Ross FJ, McCoy CO, Bumgarner R, Marrazzo JM, Fredricks DN (2012) Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria. PLoS One 7(6), e37818
13. Dumonceaux TJ, Schellenberg J, Goleski V, Hill JE, Jaoko W, Kimani J, Money D, Ball TB, Plummer FA, Severini A (2009) Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres. J Clin Microbiol 47(12):4067–4077
14. Fredricks DN (2011) Molecular methods to describe the spectrum and dynamics of the vaginal microbiota. Anaerobe 17(4):191–195
15. Bradshaw CS, Tabrizi SN, Fairley CK, Morton AN, Rudland E, Garland SM (2006) The association of Atopobium vaginae and Gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy. J Infect Dis 194(6):828–836
16. Antonio MA, Hawes SE, Hillier SL (1999) The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis 180(6):1950–1956
17. Marrazzo JM, Thomas KK, Fiedler TL, Ringwood K, Fredricks DN (2010) Risks for acquisition of bacterial vaginosis among women who report sex with women: a cohort study. PLoS One 5(6), e11139
18. Jakobsson T, Forsum U (2007) Lactobacillus iners: a marker of changes in the vaginal flora? J Clin Microbiol 45(9):3145
19. Oakley BB, Fiedler TL, Marrazzo JM, Fredricks DN (2008) Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis. Appl Environ Microbiol 74(15):4898–4909
20. Spear GT, Sikaroodi M, Zariffard MR, Landay AL, French AL, Gillevet PM (2008) Comparison of the diversity of the vaginal microbiota in HIV-infected and HIV-uninfected women with or without bacterial vaginosis. J Infect Dis 198(8):1131–1140
21. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ (2011) Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA 108(Suppl 1):4680–4687
22. Srinivasan S, Liu C, Mitchell CM, Fiedler TL, Thomas KK, Agnew KJ, Marrazzo JM, Fredricks DN (2010) Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. PLoS One 5(4), e10197
23. Forsum U, Jakobsson T, Larsson PG, Schmidt H, Beverly A, Bjornerem A, Carlsson B, Csango P, Donders G, Hay P, Ison C, Keane F, McDonald H, Moi H, Platz-Christensen JJ, Schwebke J (2002) An international study of the interobserver variation between interpretations of vaginal smear criteria of bacterial vaginosis. APMIS 110(11):811–818