Diagnostic accuracy of quantitative real-time PCR assay versus clinical and Gram stain identification of bacterial vaginosis

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 29, Issue 12, 2010, Pages 1547-1552

  Menard, J P ^a,b   Mazouni, C ^a   Fenollar, F ^b   Raoult, D ^b   Boubli, L ^a   Bretelle, F ^a,b,c  

^a HÔPITAL NORD   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

^c Centre d'Investigation Clinique   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ADULT; AMSEL CRITERIA; ARTICLE; BACTERIAL GENE; BACTERIAL LOAD; CLINICAL ARTICLE; CONTROLLED CLINICAL TRIAL; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; GARDNERELLA VAGINALIS; GRAM STAINING; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; NUGENT SCORE; PREDICTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCORING SYSTEM; SENSITIVITY AND SPECIFICITY; VAGINITIS;

ACTINOBACTERIA; ADULT; FEMALE; GARDNERELLA VAGINALIS; GENTIAN VIOLET; HUMANS; PHENAZINES; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PREGNANCY; PREGNANCY COMPLICATIONS, INFECTIOUS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; VAGINA; VAGINOSIS, BACTERIAL; YOUNG ADULT;

EID: 78651283491     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-010-1039-3     Document Type: Article

References (24)

1. JE Allsworth JF Peipert 2007 Prevalence of bacterial vaginosis: 2001-2004 National Health and Nutrition Examination Survey data Obstet Gynecol 109 114 120 10.1097/01.AOG.0000247627.84791.91 17197596 (Pubitemid 46035517)
2. PG Larsson M Bergström U Forsum B Jacobsson A Strand P Wölner-Hanssen 2005 Bacterial vaginosis. Transmission, role in genital tract infection and pregnancy outcome: an enigma APMIS 113 233 245 1:STN:280:DC%2BD2M3jtV2qtA%3D%3D 10.1111/j.1600-0463.2005.apm-01.x 15865604 (Pubitemid 40676804)
3. H Leitich H Kiss 2007 Asymptomatic bacterial vaginosis and intermediate flora as risk factors for adverse pregnancy outcome Best Pract Res Clin Obstet Gynaecol 21 375 390 10.1016/j.bpobgyn.2006.12.005 17241817 (Pubitemid 46825482)
4. U.S. Preventive Services Task Force 2008 Screening for bacterial vaginosis in pregnancy to prevent preterm delivery: U.S. Preventive Services Task Force recommendation statement Ann Intern Med 148 214 219
5. P Nygren R Fu M Freeman C Bougatsos M Klebanoff JM Guise U.S. Preventive Services Task Force 2008 Evidence on the benefits and harms of screening and treating pregnant women who are asymptomatic for bacterial vaginosis: an update review for the U.S. Preventive Services Task Force Ann Intern Med 148 220 233 18252684
6. MA Klebanoff JR Schwebke J Zhang TR Nansel KF Yu WW Andrews 2004 Vulvovaginal symptoms in women with bacterial vaginosis Obstet Gynecol 104 267 272 10.1097/01.AOG.0000134783.98382.b0 15291998 (Pubitemid 40458871)
7. PG Larsson B Carlsson L Fahråeus T Jakobsson U Forsum 2004 Diagnosis of bacterial vaginosis: need for validation of microscopic image area used for scoring bacterial morphotypes Sex Transm Infect 80 63 67 10.1136/sti.2003.006106 14755040 (Pubitemid 38221899)
8. MD Libman M Kramer R Platt Montreal Prematurity Study Group 2006 Comparison of Gram and Kopeloff stains in the diagnosis of bacterial vaginosis in pregnancy Diagn Microbiol Infect Dis 54 197 201 10.1016/j.diagmicrobio.2005. 09.017 16427240
9. McDonald HM, Brocklehurst P, Gordon A (2007) Antibiotics for treating bacterial vaginosis in pregnancy. Cochrane Database Syst Rev (1):CD000262
10. DN Fredricks TL Fiedler JM Marrazzo 2005 Molecular identification of bacteria associated with bacterial vaginosis N Engl J Med 353 1899 1911 1:CAS:528:DC%2BD2MXhtF2rsL%2FL 10.1056/NEJMoa043802 16267321 (Pubitemid 41565955)
11. DN Fredricks TL Fiedler KK Thomas BB Oakley JM Marrazzo 2007 Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis J Clin Microbiol 45 3270 3276 1:CAS:528:DC%2BD2sXht12rurbK 10.1128/JCM.01272-07 17687006 (Pubitemid 47585650)
12. E De Backer R Verhelst H Verstraelen MA Alqumber JP Burton JR Tagg M Temmerman M Vaneechoutte 2007 Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners BMC Microbiol 7 115 10.1186/1471-2180-7-115 18093311 (Pubitemid 351231099)
13. CS Bradshaw SN Tabrizi CK Fairley AN Morton E Rudland SM Garland 2006 The association of Atopobium vaginae and Gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy J Infect Dis 194 828 836 1:CAS:528:DC%2BD28XhtVamsLfJ 10.1086/506621 16941351 (Pubitemid 44344132)
14. JP Menard F Fenollar M Henry F Bretelle D Raoult 2008 Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis Clin Infect Dis 47 33 43 1:CAS:528:DC%2BD1cXosFejt7w%3D 10.1086/588661 18513147 (Pubitemid 351920584)
15. DN Fredricks TL Fiedler KK Thomas CM Mitchell JM Marrazzo 2009 Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR J Clin Microbiol 47 721 726 1:CAS:528:DC%2BD1MXktFClurg%3D 10.1128/JCM.01384-08 19144794
16. R Amsel PA Totten CA Spiegel KC Chen D Eschenbach KK Holmes 1983 Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations Am J Med 74 14 22 1:STN:280:DyaL3s%2FpslGltw%3D%3D 10.1016/0002-9343(83)91112-9 6600371 (Pubitemid 13221599)
17. RP Nugent MA Krohn SL Hillier 1991 Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation J Clin Microbiol 29 297 301 1:STN:280:DyaK3M7nvVSmsQ%3D%3D 1706728
18. The JavaStat online statistics package. Available online at: http://statpages.org/ctab2x2.html. Accessed 5 December 2009
19. U Forsum T Jakobsson PG Larsson H Schmidt A Beverly A Bjørnerem B Carlsson P Csango G Donders P Hay C Ison F Keane H McDonald H Moi JJ Platz-Christensen J Schwebke 2002 An international study of the interobserver variation between interpretations of vaginal smear criteria of bacterial vaginosis APMIS 110 811 818 1:STN:280:DC%2BD3s%2FosV2qtw%3D%3D 10.1034/j.1600-0463.2002.1101107.x 12596717 (Pubitemid 36152714)
20. UBC Bayesian Calculator Type 2. Available online at: http://spph.ubc.ca/ sites/healthcare/files/calc/bayes.html. Accessed 5 December 2009
21. BE Sha HY Chen QJ Wang MR Zariffard MH Cohen GT Spear 2005 Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women J Clin Microbiol 43 4607 4612 10.1128/JCM.43.9.4607-4612.2005 16145114 (Pubitemid 41298250)
22. CA Ison PE Hay 2002 Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics Sex Transm Infect 78 413 415 1:STN:280:DC%2BD38jhtF2quw%3D%3D 10.1136/sti.78.6.413 12473800 (Pubitemid 36020813)
23. JP Menard C Mazouni I Salem-Cherif F Fenollar D Raoult L Boubli M Gamerre F Bretelle 2010 High vaginal concentrations of Atopobium vaginae and Gardnerella vaginalis in women undergoing preterm labor Obstet Gynecol 115 134 140 10.1097/AOG.0b013e3181c391d7 20027045
24. S Srinivasan C Liu CM Mitchell TL Fiedler KK Thomas KJ Agnew JM Marrazzo DN Fredricks 2010 Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis PLoS ONE 5 e10197 10.1371/journal.pone. 0010197 20419168