Visualization of coral host–pathogen interactions using a stable GFP-labeled Vibrio coralliilyticus strain

Coral Reefs

Volumn 34, Issue 2, 2015, Pages 655-662

  Pollock, F Joseph ^a,b   Krediet, Cory J ^a,c,d   Garren, Melissa ^e   Stocker, Roman ^e   Winn, Karina ^a   Wilson, Bryan ^a   Huete Stauffer, Carla ^a,f   Willis, Bette L ^b   Bourne, David G ^a  

^a AUSTRALIAN INSTITUTE OF MARINE SCIENCE   (Australia)

^b JAMES COOK UNIVERSITY   (Australia)

^c UNIVERSITY OF FLORIDA   (United States)

^d STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e Massachusetts Institute of Technology Cambridge   (United States)

^f UNIVERSITY OF GENOA   (Italy)

Author keywords

Bacteria; Coral disease; Green fluorescent protein; Host pathogen interactions; Pathogens; Vibrio coralliilyticus

Indexed keywords

EID: 84939996881     PISSN: 07224028     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00338-015-1273-3     Document Type: Article

References (34)

1. Abrego D, Ulstrup KE, Willis BL, van Oppen MJ (2008) Species–specific interactions between algal endosymbionts and coral hosts define their bleaching response to heat and light stress. Proc R Soc B Biol Sci 275:2273–2282
2. Bao Y, Lies DP, Fu H, Roberts GP (1991) An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria. Gene 109:167–168
3. Ben-Haim Y, Rosenberg E (2002) A novel Vibrio sp. pathogen of the coral Pocillopora damicornis. Mar Biol 141:47–55
4. Ben-Haim Y, Thompson FL, Thompson CC, Cnockaert MC, Hoste B, Swings J, Rosenberg E (2003) Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornis. Int J Syst Evol Microbiol 53:309–315
5. Bourne DG, Garren M, Work TM, Rosenberg E, Smith GW, Harvell CD (2009) Microbial disease and the coral holobiont. Trends Microbiol 17:554–562
6. Brewster JD (2003) A simple micro-growth assay for enumerating bacteria. J Microbiol Methods 53:77–86
7. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802–805
8. Cohen Y, Pollock FJ, Rosenberg E, Bourne DG (2013) Phage therapy treatment of the coral pathogen Vibrio coralliilyticus. Microbiologyopen 2:64–74
9. Dunn AK, Millikan DS, Adin DM, Bose JL, Stabb EV (2006) New rfp-and pES213-derived tools for analyzing symbiotic Vibrio fischeri reveal patterns of infection and lux expression in situ. Appl Environ Microbiol 72:802–810
10. Errampalli D, Leung K, Cassidy MB, Kostrzynska M, Blears M, Lee H, Trevors JT (1999) Applications of the green fluorescent protein as a molecular marker in environmental microorganisms. J Microbiol Methods 35:187–199
11. Garren M, Son K, Raina J-B, Rusconi R, Menolascina F, Shapiro OH, Tout J, Bourne DG, Seymour JR, Stocker R (2014) A bacterial pathogen uses dimethylsulfoniopropionate as a cue to target heat-stressed corals. ISME J 8:999–1007
12. Guillard R, Ryther J (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea. Can J Microbiol 8:229–239
13. Kimes NE, Grim CJ, Johnson WR, Hasan NA, Tall BD, Kothary MH, Kiss H, Munk AC, Tapia R, Green L et al (2011) Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus. ISME J 6:835–846
14. Lambertsen L, Sternberg C, Molin S (2004) Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins. Environ Microbiol 6:726–732
15. Lichtenstein C, Brenner S (1981) Site-specific properties of Tn7 transposition into the E. coli chromosome. Mol Gen Genet 183:380–387
16. Ling SH, Wang XH, Lim TM, Leung KY (2001) Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish. FEMS Microbiol Lett 194:239–243
17. Littman RA, Bourne DG, Willis BL (2010) Responses of coral-associated bacterial communities to heat stress differ with Symbiodinium type on the same coral host. Mol Ecol 19:1978–1990
18. Meron D, Efrony R, Johnson WR, Schaefer AL, Morris PJ, Rosenberg E, Greenberg EP, Banin E (2009) Role of flagella in virulence of the coral pathogen Vibrio coralliilyticus. Appl Env Microbiol 75:5704–5707
19. Pollock FJ, Morris PJ, Willis BL, Bourne DG (2011) The urgent need for robust coral disease diagnostics. PLoS Pathog 7:e1002183
20. Pollock FJ, Wilson B, Johnson WR, Morris PJ, Willis BL, Bourne DG (2010) Phylogeny of the cosmopolitan coral pathogen Vibrio coralliilyticus. Env Microbiol Rep 2:172–178
21. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229–233
22. Sawabe T, Fukui Y, Stabb EV (2006) Simple conjugation and outgrowth procedures for tagging vibrios with GFP, and factors affecting the stable expression of the gfp tag. Lett Appl Microbiol 43:514–522
23. Shapiro OH, Fernandez VI, Garren MS, Guasto JS, Debaillon-Vesque FP, Kramarski-Winter E, Vardi A, Stocker R (2014) Vortical ciliary flows actively enhance mass transport in reef corals. Proc Natl Acad Sci 111:13391–13396
24. StatSoft Inc. (2013) STATISTICA (data analysis software system), version 12. Tulsa, USA www.statsoft.com
25. Sussman M, Willis BL, Victor S, Bourne DG (2008) Coral pathogens identified for White Syndrome (WS) epizootics in the Indo-Pacific. PLoS One 3:e2393
26. Sussman M, Mieog JC, Doyle J, Victor S, Willis BL, Bourne DG (2009) Vibrio zinc-metalloprotease causes photoinactivation of coral endosymbionts and coral tissue lesions. PLoS One 4:e4511
27. Thompson FL, Gevers D, Thompson CC, Dawyndt P, Naser S, Hoste B, Munn CB, Swings J (2005) Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Env Microbiol 71:5107–5115
28. Ushijima B, Videau P, Burger AH, Shore-Maggio A, Runyon CM, Sudek M, Aeby GS, Callahan SM (2014) Vibrio coralliilyticus Strain OCN008 is an etiological agent of acute Montipora White Syndrome. Appl Environ Microbiol 80:2102–2109
29. Valdivia RH, Hromockyj AE, Monack D, Ramakrishnan L, Falkow S (1996) Applications for green fluorescent protein (GFP) in the study of host pathogen interactions. Gene 173:47–52
30. Vidal-Dupiol J, Ladrière O, Meistertzheim A-L, Fouré L, Adjeroud M, Mitta G (2011) Physiological responses of the scleractinian coral Pocillopora damicornis to bacterial stress from Vibrio coralliilyticus. J Exp Biol 214:1533–1545
31. Vizcaino MI, Johnson WR, Kimes NE, Williams K, Torralba M, Nelson KE, Smith GW, Weil E, Moeller PD, Morris PJ (2010) Antimicrobial resistance of the coral pathogen Vibrio coralliilyticus and Caribbean sister phylotypes isolated from a diseased octocoral. Microb Ecol 59:646–657
32. Weis VM, Davy SK, Hoegh-Guldberg O, Rodriguez-Lanetty M, Pringle JR (2008) Cell biology in model systems as the key to understanding corals. Trends Ecol Evol 23:369–376
33. Windle HJ, Kelleher D (1997) Identification and characterization of a metalloprotease activity from Helicobacter pylori. Infect Immun 65:3132–3137
34. Work T, Meteyer C (2014) To understand coral disease, look at coral cells. EcoHealth. doi:10.1007/s10393-014-0931-1:1-9