Purification, refolding, and crystallization of the outer membrane protein OmpG from Escherichia coli

Methods in Enzymology

Volumn 557, Issue , 2015, Pages 149-166

  Köster, Stefan ^a,b   Van Pee, Katharina ^a   Yildiz, Özkan ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI PROTEIN; OMPG PROTEIN, E COLI; OUTER MEMBRANE PROTEIN; PORIN;

CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; ELECTROPHORESIS; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; PROTEIN REFOLDING; PROTEIN SECONDARY STRUCTURE; SIZE EXCLUSION CHROMATOGRAPHY; ULTRASTRUCTURE; X RAY CRYSTALLOGRAPHY;

BACTERIAL OUTER MEMBRANE PROTEINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CIRCULAR DICHROISM; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; PORINS; PROTEIN REFOLDING; PROTEIN STRUCTURE, SECONDARY;

EID: 84939950659     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.01.018     Document Type: Chapter

References (31)

1. W. Achouak, T. Heulin, and J.M. Pages Multiple facets of bacterial porins FEMS Microbiology Letters 199 1 2001 1 7
2. M. Behlau, D.J. Mills, H. Quader, W. Kühlbrandt, and J. Vonck Projection structure of the monomeric porin OmpG at 6 A resolution Journal of Molecular Biology 305 1 2001 71 77 10.1006/jmbi.2000.4284
3. R. Benz, K. Janko, W. Boos, and P. Lauger Formation of large, ion-permeable membrane channels by the matrix protein (porin) of Escherichia coli Biochimica et Biophysica Acta 511 3 1978 305 319 10.1016/0005-2736(78)90269-9
4. R. Benz, A. Schmid, and G.H. Vos-Scheperkeuter Mechanism of sugar transport through the sugar-specific LamB channel of Escherichia coli outer membrane The Journal of Membrane Biology 100 1 1987 21 29
5. J.M. Bolla, E. Loret, M. Zalewski, and J.M. Pages Conformational analysis of the Campylobacter jejuni porin Journal of Bacteriology 177 15 1995 4266 4271
6. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry 72 1976 248 254
7. S. Conlan, and H. Bayley Folding of a monomeric porin, OmpG, in detergent solution Biochemistry 42 31 2003 9453 9465 10.1021/bi0344228
8. S. Conlan, Y. Zhang, S. Cheley, and H. Bayley Biochemical and biophysical characterization of OmpG: A monomeric porin Biochemistry 39 39 2000 11845 11854 10.1021/bi001065h
9. R.A. Crowther, R. Henderson, and J.M. Smith MRC image processing programs Journal of Structural Biology 116 1 1996 9 16 10.1006/jsbi.1996.0003
10. M. Damaghi, C. Bippes, S. Koster, O. Yildiz, S.A. Mari, and W. Kuhlbrandt pH-dependent interactions guide the folding and gate the transmembrane pore of the beta-barrel membrane protein OmpG Journal of Molecular Biology 397 4 2010 878 882 10.1016/j.jmb.2010.02.023
11. M. Damaghi, K.T. Sapra, S. Koster, O. Yildiz, W. Kuhlbrandt, and D.J. Muller Dual energy landscape: The functional state of the beta-barrel outer membrane protein G molds its unfolding energy landscape Proteomics 10 23 2010 4151 4162 10.1002/pmic.201000241
12. D.A. Fajardo, J. Cheung, C. Ito, E. Sugawara, H. Nikaido, and R. Misra Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels Journal of Bacteriology 180 17 1998 4452 4459
13. M. Galdiero, M. Vitiello, and S. Galdiero Eukaryotic cell signaling and transcriptional activation induced by bacterial porins FEMS Microbiology Letters 226 1 2003 57 64
14. P. Goswami, C. Paulino, D. Hizlan, J. Vonck, O. Yildiz, and W. Kuhlbrandt Structure of the archaeal Na +/H + antiporter NhaP1 and functional role of transmembrane helix 1 EMBO Journal 30 2 2011 439 449 10.1038/emboj.2010.321
15. R. Henderson, J.M. Baldwin, K.H. Downing, J. Lepault, and F. Zemlin Structure of purple membrane from Halobacterium halobium - Recording, measurement and evaluation of electron micrographs at 3.5 A resolution Ultramicroscopy 19 2 1986 147 178
16. B.K. Jap, K.H. Downing, and P.J. Walian Structure of PhoE porin in projection at 3.5 A resolution Journal of Structural Biology 103 1 1990 57 63
17. W. Kabsch Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants Journal of Applied Crystallography 26 1993 795 800
18. F. Korkmaz, S. Köster, O. Yildiz, and W. Mantele The role of lipids for the functional integrity of porin: An FTIR study using lipid and protein reporter groups Biochemistry 47 46 2008 12126 12134
19. F. Korkmaz-Özkan, S. Köster, W. Kühlbrandt, W. Mäntele, and Ö. Yildiz Correlation between the OmpG secondary structure and its pH-dependent alterations monitored by FTIR Journal of Molecular Biology 401 2010 56 67 10.1016/j.jmb.2010.06.015
20. S.A. Mari, S. Koster, C.A. Bippes, O. Yildiz, W. Kuhlbrandt, and D.J. Muller pH-induced conformational change of the beta-barrel-forming protein OmpG reconstituted into native E. coli lipids Journal of Molecular Biology 396 3 2010 610 616 10.1016/j.jmb.2009.12.034
21. L. Martinez-Martinez Extended-spectrum beta-lactamases and the permeability barrier Clinical Microbiology and Infection 14 Suppl. 1 2008 82 89 10.1111/j.1469-0691.2007.01860.x
22. B. Miroux, and J.E. Walker Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels Journal of Molecular Biology 260 3 1996 289 298 10.1006/jmbi.1996.0399
23. K. Nakamura, and S. Mizushima Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins from Escherichia coli K-12 Journal of Biochemistry 80 6 1976 1411 1422
24. M. Naumann, T. Rudel, and T.F. Meyer Host cell interactions and signalling with Neisseria gonorrhoeae Current Opinion in Microbiology 2 1 1999 62 70
25. H. Nikaido Molecular basis of bacterial outer membrane permeability revisited Microbiology and Molecular Biology Reviews 67 4 2003 593 656
26. J.P. Rosenbusch Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding Journal of Biological Chemistry 249 24 1974 8019 8029
27. H.J. Sass, G. Buldt, E. Beckmann, F. Zemlin, M. van Heel, and E. Zeitler Densely packed beta-structure at the protein-lipid interface of porin is revealed by high-resolution cryo-electron microscopy Journal of Molecular Biology 209 1 1989 171 175 10.1016/0022-2836(89)90180-0
28. G.V. Subbarao, and B. van den Berg Crystal structure of the monomeric porin OmpG Journal of Molecular Biology 360 4 2006 750 759
29. J.M. Valpuesta, J.L. Carrascosa, and R. Henderson Analysis of electron microscope images and electron diffraction patterns of thin crystals of phi 29 connectors in ice Journal of Molecular Biology 240 4 1994 281 287
30. D.N. Wang, and W. Kühlbrandt High-resolution electron crystallography of light-harvesting chlorophyll a/b-protein complex in three different media Journal of Molecular Biology 217 1991 691 699 10.1016/0022-2836(91)90526-C
31. Ö. Yildiz, K.R. Vinothkumar, P. Goswami, and W. Kühlbrandt Structure of the monomeric outer-membrane porin OmpG in the open and closed conformation EMBO Journal 25 15 2006 3702 3713