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Volumn 68, Issue 4, 2014, Pages 834-841

How to Deal with PCR Contamination in Molecular Microbial Ecology

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DEOXYRIBONUCLEASE I; DITHIOTHREITOL; GATC-SPECIFIC TYPE II DEOXYRIBONUCLEASES; RIBOSOMAL SPACER DNA; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

EID: 84939879242     PISSN: 00953628     EISSN: 1432184X     Source Type: Journal    
DOI: 10.1007/s00248-014-0453-y     Document Type: Article
Times cited : (8)

References (13)
  • 2
    • 84875301338 scopus 로고    scopus 로고
    • Age-related differences in the cloacal microbiota of a wild bird species
    • PID: 23531085
    • Van Dongen WFD, White J, Brandl HB et al (2013) Age-related differences in the cloacal microbiota of a wild bird species. BMC Ecol 13:11. doi:10.1186/1472-6785-13-11
    • (2013) BMC Ecol , vol.13 , pp. 11
    • Van Dongen, W.F.D.1    White, J.2    Brandl, H.B.3
  • 3
    • 71049160836 scopus 로고    scopus 로고
    • Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood
    • PID: 18722072
    • Mühl H, Kochem A-J, Disqué C, Sakka SG (2010) Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diagn Microbiol Infect Dis 66:41–9. doi:10.1016/j.diagmicrobio.2008.07.011
    • (2010) Diagn Microbiol Infect Dis , vol.66 , pp. 41-49
    • Mühl, H.1    Kochem, A.-J.2    Disqué, C.3    Sakka, S.G.4
  • 4
    • 70349271117 scopus 로고    scopus 로고
    • Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria
    • PID: 19753123
    • Spangler R, Goddard NL, Thaler DS (2009) Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. PLoS One 4:e7010. doi:10.1371/journal.pone.0007010
    • (2009) PLoS One , vol.4 , pp. e7010
    • Spangler, R.1    Goddard, N.L.2    Thaler, D.S.3
  • 5
    • 2442636328 scopus 로고    scopus 로고
    • False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy
    • PID: 15015033, COI: 1:CAS:528:DC%2BD2cXjt1GmsLc%3D
    • Borst A, Box AT, Fluit AC (2004) False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy. Eur J Clin Microbiol Infect Dis 23:289–99. doi:10.1007/s10096-004-1100-1
    • (2004) Eur J Clin Microbiol Infect Dis , vol.23 , pp. 289-299
    • Borst, A.1    Box, A.T.2    Fluit, A.C.3
  • 6
    • 84875377102 scopus 로고    scopus 로고
    • In situ transplant analysis of free-living bacteria in a lotic ecosystem
    • PID: 23257177
    • Or A, Comay O, Gophna U (2013) In situ transplant analysis of free-living bacteria in a lotic ecosystem. Res Microbiol 164:262–9. doi:10.1016/j.resmic.2012.12.004
    • (2013) Res Microbiol , vol.164 , pp. 262-269
    • Or, A.1    Comay, O.2    Gophna, U.3
  • 7
    • 84863292680 scopus 로고    scopus 로고
    • Deep sequencing analyses of low density microbial communities: working at the boundary of accurate microbiota detection
    • PID: 22412957, COI: 1:CAS:528:DC%2BC38XktFalur8%3D
    • Biesbroek G, Sanders EAM, Roeselers G et al (2012) Deep sequencing analyses of low density microbial communities: working at the boundary of accurate microbiota detection. PLoS One 7:e32942. doi:10.1371/journal.pone.0032942
    • (2012) PLoS One , vol.7 , pp. e32942
    • Biesbroek, G.1    Sanders, E.A.M.2    Roeselers, G.3
  • 8
    • 0032834486 scopus 로고    scopus 로고
    • Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion
    • PID: 10488219, COI: 1:CAS:528:DyaK1MXmsFektbk%3D
    • Carroll NM, Adamson P, Okhravi N (1999) Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion. J Clin Microbiol 37:3402–4
    • (1999) J Clin Microbiol , vol.37 , pp. 3402-3404
    • Carroll, N.M.1    Adamson, P.2    Okhravi, N.3
  • 9
    • 77958605242 scopus 로고    scopus 로고
    • An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications
    • PID: 20927390
    • Champlot S, Berthelot C, Pruvost M et al (2010) An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications. PLoS One. doi:10.1371/journal.pone.0013042
    • (2010) PLoS One
    • Champlot, S.1    Berthelot, C.2    Pruvost, M.3
  • 10
    • 0034058067 scopus 로고    scopus 로고
    • Contamination and sensitivity issues with a real-time universal 16S rRNA PCR
    • PID: 10790092, COI: 1:CAS:528:DC%2BD3cXjsFegs78%3D
    • Corless CE, Guiver M, Borrow R, Fox AJ (2000) Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol 38:1747
    • (2000) J Clin Microbiol , vol.38 , pp. 1747
    • Corless, C.E.1    Guiver, M.2    Borrow, R.3    Fox, A.J.4
  • 11
    • 79957556739 scopus 로고    scopus 로고
    • An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents
    • PID: 21637859, COI: 1:CAS:528:DC%2BC3MXntVaisLc%3D
    • Chang S-S, Hsu H-L, Cheng J-C, Tseng C-P (2011) An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents. PLoS One 6:e20303. doi:10.1371/journal.pone.0020303
    • (2011) PLoS One , vol.6 , pp. e20303
    • Chang, S.-S.1    Hsu, H.-L.2    Cheng, J.-C.3    Tseng, C.-P.4
  • 12
    • 39949084294 scopus 로고    scopus 로고
    • Reagent decontamination to eliminate false-positives in Escherichia coli qPCR
    • PID: 18280599, COI: 1:CAS:528:DC%2BD1cXislKnt7g%3D
    • Silkie SS, Tolcher MP, Nelson KL (2008) Reagent decontamination to eliminate false-positives in Escherichia coli qPCR. J Microbiol Methods 72:275–82. doi:10.1016/j.mimet.2007.12.011
    • (2008) J Microbiol Methods , vol.72 , pp. 275-282
    • Silkie, S.S.1    Tolcher, M.P.2    Nelson, K.L.3
  • 13
    • 0035489392 scopus 로고    scopus 로고
    • Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability
    • PID: 11571146, COI: 1:CAS:528:DC%2BD3MXns1Wis7s%3D
    • Ranjard L, Poly F, Lata J et al (2001) Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability. Appl Environ Microbiol 67:4479–4487. doi:10.1128/AEM.67.10.4479
    • (2001) Appl Environ Microbiol , vol.67 , pp. 4479-4487
    • Ranjard, L.1    Poly, F.2    Lata, J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.