Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

Applied Microbiology and Biotechnology

Volumn 99, Issue 18, 2015, Pages 7549-7558

  Kitaoka, Naoki ^a,b   Wu, Yisheng ^a,c   Xu, Meimei ^a   Peters, Reuben J ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b TOHOKU UNIVERSITY   (Japan)

^c Conagen Inc ^*  (United States)

Author keywords

Antibiotic; Evolution; Labdane related diterpenoids; Metabolic engineering; Natural products; Phytoalexin

Indexed keywords

ANTIBIOTICS; BIOLOGY; BIOSYNTHESIS; ESCHERICHIA COLI; LIPIDS; METABOLIC ENGINEERING; PLANTS (BOTANY);

EVOLUTION; FUNCTIONAL EXPRESSION; LABDANE-RELATED DITERPENOIDS; NATURAL PRODUCTS; PHYTOALEXIN; RECOMBINANT EXPRESSION; RECOMBINANT EXPRESSION SYSTEM; SYNTHETIC BIOLOGY;

BIOCHEMISTRY;

2ALPHA HYDROXY ENT CASSADIENE; 2ALPHA HYDROXY ENT ISOKAURENE; 2ALPHA,3ALPHA DIHYDROXY ENT ISOKAURENE; 3ALPHA HYDROXY ENT CASSADIEN 2 ONE; CYTOCHROME P450; CYTOCHROME P450 701A8; CYTOCHROME P450 71Z6; CYTOCHROME P450 71Z7; DITERPENOID; ENT CASSADIENE 2 ONE; PIMARADIENE; UNCLASSIFIED DRUG; DITERPENE; RECOMBINANT PROTEIN;

ANTIBIOTICS; BIOENGINEERING; CATALYSIS; ENZYME ACTIVITY; GENE EXPRESSION; HYDROXYL RADICAL; METABOLISM; OPTIMIZATION; ORGANIC COMPOUND; OXYGENATION; RECOMBINATION; RICE; TERPENE;

ARTICLE; BIOSYNTHESIS; CATALYSIS; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HYDROXYLATION; METABOLIC ENGINEERING; NONHUMAN; PARALOGY; PROTEIN EXPRESSION; RICE; STEREOSPECIFICITY; SYNTHETIC BIOLOGY; ENZYMOLOGY; GENETICS; METABOLISM; ORYZA;

ESCHERICHIA COLI;

CYTOCHROME P-450 ENZYME SYSTEM; DITERPENES; ESCHERICHIA COLI; ORYZA; RECOMBINANT PROTEINS;

EID: 84939572274     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6496-2     Document Type: Article

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