Description of an original integron encompassing blaVIM-2, qnrVC1 and genes encoding bacterial group II intron proteins in Pseudomonas aeruginosa

Journal of Antimicrobial Chemotherapy

Volumn 70, Issue 8, 2015, Pages 2237-2240

  Belotti, P T ^a   Thabet, L ^b   Laffargue, A ^a   Andre C ^a   Coulange Mayonnove, L ^a   Arpin, C ^a   Messadi, A ^b   M'Zali, F ^a   Quentin, C ^a   Dubois, V ^a  

^a University Victor Segalen   (France)

^b Hopital Aziza Othmana   (Tunisia)

Author keywords

Burn unit; Imipenem resistance; P. aeruginosa; VIM 2 carbapenemase

Indexed keywords

IMIPENEM; ANTIINFECTIVE AGENT; BETA LACTAMASE; VIM-2 BETA-LACTAMASE;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CLONING; CONTENT ANALYSIS; DNA SEQUENCE; ESCHERICHIA COLI; GENE CASSETTE; INTEGRON; INTRON; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOBACTERIUM PHOSPHOREUM; PLASMID; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; QNRVC1 GENE; SKIN INJURY; VIBRIO PARAHAEMOLYTICUS; VIBRIONACEAE; ANTIBIOTIC RESISTANCE; BACTERIUM CONJUGATION; BACTERIUM TRANSFORMATION; BURN UNIT; BURNS; COMPLICATION; ENDEMIC DISEASE; ENZYMOLOGY; GENE EXPRESSION PROFILING; GENETICS; HORIZONTAL GENE TRANSFER; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; PSEUDOMONAS INFECTION; TUNISIA;

ANTI-BACTERIAL AGENTS; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; BURN UNITS; BURNS; CONJUGATION, GENETIC; ENDEMIC DISEASES; GENE EXPRESSION PROFILING; GENE TRANSFER, HORIZONTAL; GENES, BACTERIAL; HUMANS; IMIPENEM; INTEGRONS; INTRONS; MOLECULAR SEQUENCE DATA; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, BACTERIAL; TUNISIA;

EID: 84939476941     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkv103     Document Type: Article

References (20)

1. Branski LK, Al-Mousawi A, Rivero H et al. Emerging infections in burns. Surg Infect (Larchmt) 2009; 10: 389-97.
2. Miriagou V, Cornaglia G, Edelstein M et al. Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. Clin Microb Infect 2010; 16: 112-22.
3. Thabet L, Zoghlami A, Boukadida J et al. Comparative study of antibiotic resistance in bacteria isolated from burned patients during two periods (2005-2008, 2008-2011) and in two hospitals (Hospital Aziza Othmana, Trauma and Burn Center). Tunis Med 2013; 91: 134-8.
4. Dubois V, Poirel L, Marie C et al. Molecular characterization of a novel class 1 integron containing blaGES-1 and a fused product of aac3-Ib/ aac6'-Ib' gene cassettes in Pseudomonas aeruginosa. Antimicrob Agents Chemother 2002; 46: 638-45.
5. Carrer A, Poirel L, Eraksoy H et al. Spread of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in Istanbul, Turkey. Antimicrob Agents Chemother 2008; 52: 2950-4.
6. Dubois V, Arpin C, Quentin C et al. Decreased susceptibility to cefepime in a clinical strain of Escherichia coli related to plasmid-and integronencoded OXA-30 β-lactamase. Antimicrob Agents Chemother 2003; 47: 2380-1.
7. Ploy MC, Denis F, Courvalin P et al. Molecular characterization of integrons in Acinetobacter baumannii: description of a hybrid class 2 integron. Antimicrob Agents Chemother 2000; 44: 2684-8.
8. Poirel L, Schrenzel J, Cherkaoui A et al. Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland. J Antimicrob Chemother 2011; 66: 1730-3.
9. Akasaka T, Tanaka M, Yamaguchi A et al. Type II topoisomerase mutations in fluoroquinolone-resistant clinical strains of Pseudomonas aeruginosa isolated in 1998 and 1999: role of target enzyme in mechanism of fluoroquinolone resistance. Antimicrob Agents Chemother 2001; 45: 2263-8.
10. Hawkey PM, Jones AM. The changing epidemiology of resistance. J Antimicrob Chemother 2009; 64 Suppl 1: i3-10.
11. Walsh TR. Emerging carbapenemases: a global perspective. Int J Antimicrob Agents 2010; 36 Suppl 3: S8-14.
12. Hammami S, Boutiba-Ben Boubaker I, Ghozzi R et al. Nosocomial outbreak of imipenem-resistant Pseudomonas aeruginosa producing VIM-2 metallo-β-lactamase in a kidney transplantation unit. Diagn Pathol 2011; 6: 106.
13. Ktari S, Mnif B, Znazen A et al. Diversity of β-lactamases in Pseudomonas aeruginosa isolates producing metallo-β-lactamase in two Tunisian hospitals. Microb Drug Resist 2011; 17: 25-30.
14. Mansour W, Poirel L, Bettaieb D et al. Metallo-β-lactamase-producing Pseudomonas aeruginosa isolates in Tunisia. Diagn Microbiol Infect Dis 2009; 64: 458-61.
15. da Fonseca EL, Dos Santos Freitas F, Vieira VV et al. Newqnr gene cassettes associated with superintegron repeats in Vibrio cholerae O1. Emerg Infect Dis 2008; 14: 1129-31.
16. Cattoir V, Nordmann P. Plasmid-mediated quinolone resistance in Gram-negative bacterial species: an update. Curr Med Chem 2009; 16: 1028-46.
17. da Fonseca EL, Vicente AC. Functional characterization of a cassettespecific promoter in the class 1 integron-associated qnrVC1 gene. Antimicrob Agents Chemother 2012; 56: 3392-4.
18. Leon G, Roy PH. Potential role of group IIC-attC introns in integron cassette formation. J Bacteriol 2009; 191: 6040-51.
19. Rodriguez-Martinez JM, Nordmann P, Poirel L. Group IIC intron with an unusual target of integration in Enterobacter cloacae. J Bacteriol 2012; 194: 150-60.
20. Rodriguez-Martinez JM, Cano ME, Velasco C et al. Plasmid-mediated quinolone resistance: an update. J Infect Chemother 2011; 17: 149-82.