High-yield isolation of extracellular vesicles using aqueous two-phase system

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Shin, Hyunwoo ^a   Han, Chungmin ^a   Labuz, Joseph M ^b   Kim, Jiyoon ^a   Kim, Jongmin ^a   Cho, Siwoo ^a   Gho, Yong Song ^a   Takayama, Shuichi ^b   Park, Jaesung ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEXTRAN; MACROGOL DERIVATIVE; WATER;

ANIMAL; C57BL MOUSE; CELL FRACTIONATION; CELL SEPARATION; CHEMISTRY; EVALUATION STUDY; EXOSOME; EXPERIMENTAL NEOPLASM; MOUSE; PHASE TRANSITION; PROCEDURES; SOLID PHASE EXTRACTION; TUMOR CELL LINE; ULTRASTRUCTURE;

ANIMALS; CELL FRACTIONATION; CELL LINE, TUMOR; CELL SEPARATION; DEXTRANS; EXTRACELLULAR VESICLES; MICE; MICE, INBRED C57BL; NEOPLASMS, EXPERIMENTAL; PHASE TRANSITION; POLYETHYLENE GLYCOLS; SOLID PHASE EXTRACTION; WATER;

EID: 84939426434     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep13103     Document Type: Article

References (38)

1. D'Souza-Schorey, C. & Clancy, J. W. Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers. Genes & development 26, 1287-1299 (2012).
2. Nilsson, J. et al. Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer. Br J Cancer 100, 1603-1607 (2009).
3. Skog, J. et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10, 1470-1476, http://www.nature.com/ncb/journal/v10/n12/suppinfo/ncb1800-S1.html (2008).
4. Taylor, D. D. & Gercel-Taylor, C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecologic Oncology 110, 13-21, http://dx.doi.org/10.1016/j.ygyno.2008.04.033 (2008).
5. Witwer, K. W. et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. Journal of Extracellular Vesicles 2, doi: 10.3402/jev.v2i0.20360 (2013).
6. Thery, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Current Protocols in Cell Biology. 3.22. 21-23.22. 29 (2006).
7. Raposo, G. et al. B lymphocytes secrete antigen-presenting vesicles. The Journal of experimental medicine 183, 1161-1172 (1996).
8. Lamparski, H. G. et al. Production and characterization of clinical grade exosomes derived from dendritic cells. Journal of immunological methods 270, 211-226 (2002).
9. Davies, R. T. et al. Microfluidic filtration system to isolate extracellular vesicles from blood. Lab Chip 12, 5202-5210 (2012).
10. Gyorgy, B. et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 117, e39-e48 (2011).
11. Wubbolts, R. et al. Proteomic and Biochemical Analyses of Human B Cell-derived Exosomes POTENTIAL IMPLICATIONS FOR THEIR FUNCTION AND MULTIVESICULAR BODY FORMATION. J Biol Chem 278, 10963-10972 (2003).
12. Rabesandratana, H., Toutant, J.-P., Reggio, H. & Vidal, M. Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during in vitro maturation of reticulocytes. Blood 91, 2573-2580 (1998).
13. Clayton, A. et al. Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry. Journal of immunological methods 247, 163-174 (2001).
14. Rana, S., Yue, S., Stadel, D. & Zoller, M. Toward tailored exosomes: the exosomal tetraspanin web contributes to target cell selection. The international journal of biochemistry & cell biology 44, 1574-1584 (2012).
15. Tauro, B. J. et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 56, 293-304 (2012).
16. Ott, D. E. in HIV Protocols 15-25 (Springer, 2009).
17. Coren, L. V., Shatzer, T. & Ott, D. E. CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway. Retrovirology 5, 64 (2008).
18. Chen, C. et al. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip 10, 505-511 (2010).
19. Alvarez, M. L., Khosroheidari, M., Ravi, R. K. & DiStefano, J. K. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int 82, 1024-1032 (2012).
20. Vlassov, A., Li, M., Zeringer, E. & Conrad, R. Inventors; Life Technologies Corporation. Methods and compositions for exosome isolation. United States patent US 8, 901, 284. 2014 Dec 2.
21. Atha, D. H. & Ingham, K. C. Mechanism of precipitation of proteins by polyethylene glycols. Analysis in terms of excluded volume. J Biol Chem 256, 12108-12117 (1981).
22. Albertsson, P.-A. Particle fractionation in liquid two-phase systems The composition of some phase systems and the behaviour of some model particles in them application to the isolation of cell walls from microorganisms. Biochimica et biophysica acta 27, 378-395 (1958).
23. Walter, H., Krob, E. J. & Brooks, D. E. Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems. Biochemistry 15, 2959-2964 (1976).
24. Johansson, G. Studies on aqueous dextran-poly (ethylene glycol) two-phase systems containing charged poly (ethylene glycol) I. Partition of albumins. Biochimica et Biophysica Acta (BBA)-General Subjects 222, 381-389 (1970).
25. He, C., Li, S., Liu, H., Li, K. & Liu, F. Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt. J Chromatogr A 1082, 143-149 (2005).
26. Lantz, P.-G., Tjerneld, F., Borch, E., Hahn-Hagerdal, B. & Radstrom, P. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method. Appl Environ Microb 60, 3416-3418 (1994).
27. Walter, H. Partitioning In Aqueous Two-Phase System: Theory, Methods, Uses, And Applications To Biotechnology. (Elsevier, 1986).
28. Johansson, H. O., Karlstrom, G., Tjerneld, F. & Haynes, C. A. Driving forces for phase separation and partitioning in aqueous two-phase systems. J Chromatogr B 711, 3-17 (1998).
29. Kharaziha, P., Ceder, S., Li, Q. & Panaretakis, T. Tumor cell-derived exosomes: a message in a bottle. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer 1826, 103-111 (2012).
30. Mears, R. et al. Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Proteomics 4, 4019-4031 (2004).
31. Yu, X., Harris, S. L. & Levine, A. J. The regulation of exosome secretion: a novel function of the p53 protein. Cancer Res 66, 4795-4801 (2006).
32. Gromov, P. et al. Tumor interstitial fluid-A treasure trove of cancer biomarkers. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1834, 2259-2270 (2013).
33. Murphy, G. P. et al. Measurement of serum prostate-specific membrane antigen, a new prognostic marker for prostate cancer. Urology 51, 89-97 (1998).
34. Celis, J. E. et al. Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment A Novel Resource for Biomarker and Therapeutic Target Discovery. Molecular & Cellular Proteomics 3, 327-344 (2004).
35. Runyon, B. A. Cardiac ascites: a characterization. Journal of clinical gastroenterology 10, 410-412 (1988).
36. Savory, J., Pu, P. H. & Sunderman, F. W. A biuret method for determination of protein in normal urine. Clinical chemistry 14, 1160-1171 (1968).
37. Harrison, P., Gardiner, C. & Sargent, I. L. Extracellular Vesicles in Health and Disease. (CRC Press, 2014).
38. Arneson, W. L. & Brickell, J. M. Clinical Chemistry: a laboratory perspective. (FA Davis, 2007).