Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells

Methods in Molecular Biology

Volumn 1307, Issue , 2015, Pages 329-343

  Chambers, Stuart M ^a   Mica, Yvonne ^a   Lee, Gabsang ^a   Studer, Lorenz ^a   Tomishima, Mark J ^a  

^a NONE

Author keywords

Disease modeling; Dual SMAD inhibition; Human pluripotent stem cells; Melanocyte; Neural crest; Peripheral sensory neurons

Indexed keywords

6 [[2 [[4 (2,4 DICHLOROPHENYL) 5 (4 METHYL 1H IMIDAZOL 2 YL) 2 PYRIMIDINYL]AMINO]ETHYL]AMINO]NICOTINONITRILE; ACTIVIN; PROTEIN NODAL; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA; WNT PROTEIN; WNT3 PROTEIN;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL DIFFERENTIATION; CELL FATE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; HUMAN; HUMAN CELL; MELANOCYTE; MOUSE; NEURAL CREST; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SENSORY NERVE CELL; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL COUNT; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL LINEAGE; CYTOLOGY; METABOLISM; PROCEDURES;

ANIMALS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; HUMANS; MELANOCYTES; MICE; NEURAL CREST; PLURIPOTENT STEM CELLS; SENSORY RECEPTOR CELLS; SMAD PROTEINS; WNT PROTEINS;

EID: 84938840735     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651_2013_59     Document Type: Article

References (16)

1. Rp B (1974) The neurocristopathies: a unifying concept fo disease arising in neural crest development. Hum Pathol 5(4):409–429
2. Chambers SM, Qi Y, Mica Y, Lee G, Zhang XJ, Niu L, Bilsland J, Cao L, Stevens E, Whiting P, Shi SH, Studer L (2012) Combined smallmolecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat Biotechnol 30 (7):715–720. doi:10.1038/nbt.2249
3. Lee G, Kim H, Elkabetz Y, Al Shamy G, Panagiotakos G, Barberi T, Tabar V, Studer L (2007) Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. Nat Biotechnol 25 (12):1468–1475. doi:10.1038/nbt1365
4. Lee G, Papapetrou EP, Kim H, Chambers SM, Tomishima MJ, Fasano CA, Ganat YM, Menon J, Shimizu F, Viale A, Tabar V, Sadelain M, Studer L (2009) Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Nature 461 (7262):402–406. doi:10.1038/nature08320
5. Lee G, Ramirez CN, Kim H, Zeltner N, Liu B, Radu C, Bhinder B, Kim YJ, Choi IY, Mukherjee-Clavin B, Djaballah H, Studer L (2012) Large-scale screening using familial dysautonomia induced pluripotent stem cells identifies compounds that rescue IKBKAP expression. Nat Biotechnol 30 (12):1244–1248. doi:10.1038/nbt.2435
6. Menendez L, Yatskievych TA, Antin PB, Dalton S. (2011) Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells. Proc Natl Acad Sci U S A 108(48):19240–19245. doi:10.1073/pnas.1113746108
7. Mica Y, Lee G, Chambers SM, Tomishima MJ, Studer L (2013) Modeling neural crest induction, melanocyte specification, and diseaserelated pigmentation defects in hESCs and patient-specific iPSCs. Cell Rep 3 (4):1140–1152. doi:10.1016/j.celrep.2013.03.025
8. Jiang X, Gwye Y, McKeown SJ, Bronner-Fraser M, Lutzko C, Lawlor ER (2009) Isolation and characterization of neural crest stemcells derived from in vitro-differentiated human embryonic stem cells. Stem Cell Dev 18(7):1059–1070. doi:10.1089/scd.2008.0362
9. Kawasaki H, Mizuseki K, Nishikawa S, Kane ko S, Kuwana Y, Nakanishi S, Nishikawa SI, Sasai Y (2000) Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28(1):31–40
10. Barberi T, Klivenyi P, Calingasan NY, Lee H, Kawamata H, Loonam K, Perrier AL, Bruses J, Rubio ME, Topf N, Tabar V, Harrison NL, Beal MF, Moore MA, Studer L. (2003) Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice. Nat Biotechnol 21(10):1200–1207. doi:10.1038/nbt870
11. Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L (2008) Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Gene Dev 22(2):152–165. doi:10.1101/gad.1616208
12. Zhang SC, Wernig M, Duncan ID, Brustle O, Thomson JA (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 19 (12):1129–1133. doi:10.1038/nbt1201-1129
13. Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27(3):275–280. doi:10.1038/nbt.1529
14. Sun L, Tran N, Liang C, Tang F, Rice A, Schreck R, Waltz K, Shawver LK, McMahon G, Tang C (1999) Design, synthesis, and evaluations of substituted 3-[(3- or 4-carboxyethylpyrrol-2-yl)methylidenyl]indolin-2-ones as inhibitors of VEGF, FGF, and PDGF receptor tyrosine kinases. J Med Chem 42 (25):5120–5130
15. Dovey HF, John V, Anderson JP, Chen LZ, de Saint AP, Fang LY, Freedman SB, Folmer B, Goldbach E, Holsztynska EJ, Hu KL, Johnson-Wood KL, Kennedy SL, Kholodenko D, Knops JE, Latimer LH, Lee M, Liao Z, Lieberburg IM, Motter RN, Mutter LC, Nietz J, Quinn KP, Sacchi KL, Seubert PA, Shopp GM, Thorsett ED, Tung JS, Wu J, Yang S, Yin CT, Schenk DB, May PC, Altstiel LD, Bender MH, Boggs LN, Britton TC, Clemens JC, Czilli DL, Dieckman-McGinty DK, Droste JJ, Fuson KS, Gitter BD, Hyslop PA, Johnstone EM, Li WY, Little SP, Mabr yTE, Miller FD, Audia JE (2001) Functional gamma-secretase inhibitors reduce betaamyloid peptide levels in brain. J Neurochem 76(1):173–181
16. Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S, Muguruma K, Sasai Y (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25(6):681–686. doi:10.1038/nbt1310