The dynamics of Chinese variant porcine epidemic diarrhea virus production in Vero cells and intestines of 2-day old piglets

Virus Research

Volumn 208, Issue , 2015, Pages 82-88

  Wang, Yanhui ^b   Gao, Xiaojing ^b   Yao, Yali ^b   Zhang, Yunjing ^b   Lv, Chaochao ^b   Sun, Zhe ^b   Wang, Yuzhou ^b   Jia, Xiangrui ^b   Zhuang, Jinshan ^b   Xiao, Yan ^b   Li, Xiangdong ^b   Tian, Kegong ^a,b  

^a HENAN AGRICULTURAL UNIVERSITY   (China)

^b National Research Center for Veterinary Medicine   (China)

Author keywords

Dynamics of virus production; Immunoperoxidase monolayer assay; Porcine epidemic diarrhea virus; Serial propagation in cell culture

Indexed keywords

PEROXIDASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL GROWTH; CHINA; CONTROLLED STUDY; GENE SEQUENCE; INTESTINE; NONHUMAN; PHYLOGENY; PIGLET; PORCINE EPIDEMIC DIARRHEA VIRUS; PRIORITY JOURNAL; VERO CELL LINE; VIRUS ISOLATION; VIRUS REPLICATION; VIRUS STRAIN; VIRUS TITRATION; AMINO ACID SEQUENCE; ANIMAL; CHLOROCEBUS AETHIOPS; CLASSIFICATION; CORONAVIRUS INFECTIONS; DIARRHEA; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PIG; SEQUENCE ALIGNMENT; SWINE DISEASES; VETERINARY; VIROLOGY;

ANIMALIA; PORCINE EPIDEMIC DIARRHEA VIRUS; SUIDAE; SUS;

AMINO ACID SEQUENCE; ANIMALS; CERCOPITHECUS AETHIOPS; CHINA; CORONAVIRUS INFECTIONS; DIARRHEA; INTESTINES; MOLECULAR SEQUENCE DATA; PHYLOGENY; PORCINE EPIDEMIC DIARRHEA VIRUS; SEQUENCE ALIGNMENT; SWINE; SWINE DISEASES; VERO CELLS;

EID: 84938688779     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2015.06.009     Document Type: Article

References (19)

1. Chen Q., Li G., Stasko J., Thomas J.T., Stensland W.R., Pillatzki A.E., Gauger P.C., Schwartz K.J., Madson D., Yoon K.J., Stevenson G.W., Burrough E.R., Harmon K.M., Main R.G., Zhang J. Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States. J. Clin. Microbiol. 2014, 52:234-243.
2. Hofmann M., Wyler R. Propagation of the virus of porcine epidemic diarrhea in cell culture. J. Clin. Microbiol. 1988, 26:2235-2239.
3. Huang Y.W., Dickerman A.W., Pineyro P., Li L., Fang L., Kiehne R., Opriessnig T., Meng X.J. Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. MBio 2013, 4. e00737-13.
4. International Committee on Taxonomy of Viruses Virus taxonomy: classification and nomenclature of viruses. Ninth Report of the International Committee on Taxonomy of Viruses 2012, Elsevier Academic Press, San Diego. A.M.Q. King, M.J. Adams, E.B. Carstens, E.J. Lefkowitz (Eds.).
5. Kim O., Chae C. Experimental infection of piglets with a Korean strain of porcine epidemic diarrhoea virus. J. Comp. Pathol. 2003, 129:55-60.
6. Kusanagi K., Kuwahara H., Katoh T., Nunoya T., Ishikawa Y., Samejima T., Tajima M. Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate. J. Vet. Med. Sci. 1992, 54:313-318.
7. Kweon C.H., Kwon B.J., Lee J.G., Kwon G.O., Kang Y.B. Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate. Vaccine 1999, 17:2546-2553.
8. Madson D.M., Magstadt D.R., Arruda P.H., Hoang H., Sun D., Bower L.P., Bhandari M., Burrough E.R., Gauger P.C., Pillatzki A.E., Stevenson G.W., Wilberts B.L., Brodie J., Harmon K.M., Wang C., Main R.G., Zhang J., Yoon K.J. Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs. Vet. Microbiol. 2014, 174:60-68.
9. Oka T., Saif L.J., Marthaler D., Esseili M.A., Meulia T., Lin C.M., Vlasova A.N., Jung K., Zhang Y., Wang Q. Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene. Vet. Microbiol. 2014, 173:258-269.
10. Park S.J., Song D.S., Ha G.W., Park B.K. Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13. Virus Genes 2007, 35:55-64.
11. Pensaert M.B., de Bouck P. A new coronavirus-like particle associated with diarrhea in swine. Arch. Virol. 1978, 58:243-247.
12. Reed L.J., Muench H. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 1938, 27:493-497.
13. Retief J.D. Phylogenetic analysis using PHYLIP. Methods Mol. Biol. 2000, 132:243-258.
14. Saif L., Pensaert M.B., Sestak K., Yeo S.G., Jung K. Coronaviruses. Disease of Swine 2012, 501-524. Wiley-Blackwell.
15. Shibata I., Tsuda T., Mori M., Ono M., Sueyoshi M., Uruno K. Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet. Microbiol. 2000, 72:173-182.
16. Song D., Huang D., Peng Q., Huang T., Chen Y., Zhang T., Nie X., He H., Wang P., Liu Q., Tang Y. Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea viruses associated with outbreaks of severe diarrhea in piglets in Jiangxi, China 2013. PLOS ONE 2015, 10:e0120310.
17. Song D., Park B. Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes 2012, 44:167-175.
18. Sun R.Q., Cai R.J., Chen Y.Q., Liang P.S., Chen D.K., Song C.X. Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg. Infect. Dis. 2012, 18:161-163.
19. Wang J., Zhao P., Guo L., Liu Y., Du Y., Ren S., Li J., Zhang Y., Fan Y., Huang B., Liu S., Wu J. Porcine epidemic diarrhea virus variants with high pathogenicity, China. Emerg. Infect. Dis. 2013, 19:2048-2049.