Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene

Veterinary Microbiology

Volumn 173, Issue 3-4, 2014, Pages 258-269

  Oka, Tomoichiro ^a,b   Saif, Linda J ^a   Marthaler, Douglas ^c   Esseili, Malak A ^a   Meulia, Tea ^d   Lin, Chun Ming ^a   Vlasova, Anastasia N ^a   Jung, Kwonil ^a   Zhang, Yan ^e   Wang, Qiuhong ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^c UNIVERSITY OF MINNESOTA   (United States)

^d OHIO STATE UNIVERSITY   (United States)

^e Animal Disease Diagnostic Laboratory   (United States)

Author keywords

Cell culture isolation; Complete genome analysis; Large deletion in S protein; PEDV variants; Porcine epidemic diarrhea virus (PEDV)

Indexed keywords

ANTIVIRUS AGENT; GUANINE NUCLEOTIDE BINDING PROTEIN; LIVE VACCINE; TRYPSIN; VIRUS ANTIGEN; VITRONECTIN; CORONAVIRUS SPIKE GLYCOPROTEIN; PRIMER DNA;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CULTURE; CELL ISOLATION; ENTERIC VIRUS; EPIDEMIC; GENE DELETION; GNOTOBIOTICS; IN VITRO STUDY; INDEL MUTATION; MICROBIAL DIVERSITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PORCINE EPIDEMIC DIARRHEA VIRUS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEQUENCE ANALYSIS; VERO CELL LINE; VIRAL GENETICS; VIRUS ISOLATION; VIRUS SPIKE; ANIMAL; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTIONS; CULTURE TECHNIQUE; DNA SEQUENCE; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC VARIABILITY; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PATHOGENICITY; PIG; SPECIES DIFFERENCE; SWINE DISEASES; TRANSMISSION ELECTRON MICROSCOPY; UNITED STATES; VETERINARY; VIROLOGY; VIRULENCE;

ANIMALS; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CERCOPITHECUS AETHIOPS; CORONAVIRUS INFECTIONS; DNA PRIMERS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENETIC VARIATION; MICROSCOPY, ELECTRON, TRANSMISSION; MIDWESTERN UNITED STATES; MOLECULAR SEQUENCE DATA; PHYLOGENY; PORCINE EPIDEMIC DIARRHEA VIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; SPECIES SPECIFICITY; SPIKE GLYCOPROTEIN, CORONAVIRUS; SWINE; SWINE DISEASES; VERO CELLS; VIRULENCE;

EID: 84908191530     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2014.08.012     Document Type: Article

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