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Clinical and Laboratory Standards Institute, Wayne: This guidance document provides a comprehensive international consensus overview of IgE antibody analyses, with a focus on the strengths and limitations of singleplex autoanalyzers and multiplex assays. It examines the current state of assay technology, the appropriate biological specimens, practical methods for validation of allergen and immunological reagents, diagnostic allergy laboratory quality control strategies, guidelines for clearance of allergen-containing reagents, and the brief examination of the clinical interpretation of IgE antibody results
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Caution: the Phadia hazelnut ImmunoCAP (F17) has been supplemented with recombinant Cor a 1 and now detects Bet v 1-specific IgE, which leads to elevated values for persons with birch pollen allergy
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