Quantification of apoptosis and necroptosis at the single cell level by a combination of Imaging Flow Cytometry with classical Annexin V/propidium iodide staining

Journal of Immunological Methods

Volumn 423, Issue , 2015, Pages 99-103

  Pietkiewicz, Sabine ^a   Schmidt, Jörn H ^a   Lavrik, Inna N ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Annexin V propidium iodide; Apoptosis; Death receptor; Imaging flow cytometry; Necroptosis

Indexed keywords

DEATH RECEPTOR; LIPOCORTIN 5; PROPIDIUM IODIDE; PHOSPHATIDYLSERINE;

APOPTOSIS; ARTICLE; CELL DEATH; FLOW CYTOMETER; FLOW CYTOMETRY; IMAGE CYTOMETRY; IMAGING FLOW CYTOMETRY; JURKAT CELL LINE; NECROPTOSIS; PRIORITY JOURNAL; STAINING; SYSTEMS BIOLOGY; HUMAN; METABOLISM; NECROSIS; PATHOLOGY; PHYSIOLOGY; PROCEDURES; SINGLE CELL ANALYSIS; TUMOR CELL LINE;

ANNEXIN A5; APOPTOSIS; CELL DEATH; CELL LINE, TUMOR; FLOW CYTOMETRY; HUMANS; IMAGE CYTOMETRY; JURKAT CELLS; NECROSIS; PHOSPHATIDYLSERINES; PROPIDIUM; SINGLE-CELL ANALYSIS; STAINING AND LABELING;

EID: 84938207581     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2015.04.025     Document Type: Article

References (17)

1. Cho Y., Challa S., Chan F.K. A RNA interference screen identifies RIP3 as an essential inducer of TNF-induced programmed necrosis. Adv. Exp. Med. Biol. 2011, 691:589.
2. Degterev A., Huang Z., Boyce M., Li Y., Jagtap P., Mizushima N., Cuny G.D., Mitchison T.J., Moskowitz M.A., Yuan J. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat. Chem. Biol. 2005, 1:112.
3. Degterev A., Hitomi J., Germscheid M., Ch'en I.L., Korkina O., Teng X., Abbott D., Cuny G.D., Yuan C., Wagner G., Hedrick S.M., Gerber S.A., Lugovskoy A., Yuan J. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat. Chem. Biol. 2008, 4:313.
4. Galluzzi L., Vanden Berghe T., Vanlangenakker N., Buettner S., Eisenberg T., Vandenabeele P., Madeo F., Kroemer G. Programmed necrosis from molecules to health and disease. Int. Rev. Cell Mol. Biol. 2011, 289:1.
5. Galluzzi L., Bravo-San Pedro J.M., Vitale I., Aaronson S.A., Abrams J.M., Adam D., Alnemri E.S., Altucci L., Andrews D., Annicchiarico-Petruzzelli M., Baehrecke E.H., Bazan N.G., Bertrand M.J., Bianchi K., Blagosklonny M.V., Blomgren K., Borner C., Bredesen D.E., Brenner C., Campanella M., Candi E., Cecconi F., Chan F.K., Chandel N.S., Cheng E.H., Chipuk J.E., Cidlowski J.A., Ciechanover A., Dawson T.M., Dawson V.L., De L.V., De M.R., Debatin K.M., Di D.N., Dixit V.M., Dynlacht B.D., El-Deiry W.S., Fimia G.M., Flavell R.A., Fulda S., Garrido C., Gougeon M.L., Green D.R., Gronemeyer H., Hajnoczky G., Hardwick J.M., Hengartner M.O., Ichijo H., Joseph B., Jost P.J., Kaufmann T., Kepp O., Klionsky D.J., Knight R.A., Kumar S., Lemasters J.J., Levine B., Linkermann A., Lipton S.A., Lockshin R.A., Lopez-Otin C., Lugli E., Madeo F., Malorni W., Marine J.C., Martin S.J., Martinou J.C., Medema J.P., Meier P., Melino S., Mizushima N., Moll U., Munoz-Pinedo C., Nunez G., Oberst A., Panaretakis T., Penninger J.M., Peter M.E., Piacentini M., Pinton P., Prehn J.H., Puthalakath H., Rabinovich G.A., Ravichandran K.S., Rizzuto R., Rodrigues C.M., Rubinsztein D.C., Rudel T., Shi Y., Simon H.U., Stockwell B.R., Szabadkai G., Tait S.W., Tang H.L., Tavernarakis N., Tsujimoto Y., Vanden Berghe T., Vandenabeele P., Villunger A., Wagner E.F., Walczak H., White E., Wood W.G., Yuan J., Zakeri Z., Zhivotovsky B., Melino G., Kroemer G. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015. Cell Death Differ. 2015, 22(1):58-73.
6. George T.C., Basiji D.A., Hall B.E., Lynch D.H., Ortyn W.E., Perry D.J., Seo M.J., Zimmerman C.A., Morrissey P.J. Distinguishing modes of cell death using the ImageStream multispectral imaging flow cytometer. Cytometry A 2004, 59:237.
7. Krammer P.H., Arnold R., Lavrik I.N. Life and death in peripheral T cells. Nat. Rev. Immunol. 2007, 7:532.
8. Lavrik I.N., Krammer P.H. Regulation of CD95/Fas signaling at the DISC. Cell Death Differ. 2012, 19:36.
9. Makarov R., Geserick P., Feoktistova M., Leverkus M. Cell death in the skin: how to study its quality and quantity?. Methods Mol. Biol. 2013, 961:201.
10. Moriwaki K., Chan F.K. RIP3: a molecular switch for necrosis and inflammation. Genes Dev. 2013, 27:1640.
11. Ofengeim D., Yuan J. Regulation of RIP1 kinase signalling at the crossroads of inflammation and cell death. Nat. Rev. Mol. Cell Biol. 2013, 14:727.
12. Poon I.K., Lucas C.D., Rossi A.G., Ravichandran K.S. Apoptotic cell clearance: basic biology and therapeutic potential. Nat. Rev. Immunol. 2014, 14:166.
13. Schleich K., Warnken U., Fricker N., Ozturk S., Richter P., Kammerer K., Schnolzer M., Krammer P.H., Lavrik I.N. Stoichiometry of the CD95 death-inducing signaling complex: experimental and modeling evidence for a death effector domain chain model. Mol. Cell 2012, 47:306.
14. Vanden Berghe T., Vanlangenakker N., Parthoens E., Deckers W., Devos M., Festjens N., Guerin C.J., Brunk U.T., Declercq W., Vandenabeele P. Necroptosis, necrosis and secondary necrosis converge on similar cellular disintegration features. Cell Death Differ. 2010, 17:922.
15. Vanden Berghe T., Grootjans S., Goossens V., Dondelinger Y., Krysko D.V., Takahashi N., Vandenabeele P. Determination of apoptotic and necrotic cell death in vitro and in vivo. Methods 2013, 61:117.
16. Vandenabeele P., Galluzzi L., Vanden Berghe T., Kroemer G. Molecular mechanisms of necroptosis: an ordered cellular explosion. Nat. Rev. Mol. Cell Biol. 2010, 11:700.
17. Wang H., Sun L., Su L., Rizo J., Liu L., Wang L.F., Wang F.S., Wang X. Mixed lineage kinase domain-like protein MLKL causes necrotic membrane disruption upon phosphorylation by RIP3. Mol. Cell 2014, 54:133.