Axonal actin in action: Imaging actin dynamics in neurons

Methods in Cell Biology

Volumn 131, Issue , 2016, Pages 91-106

  Ladt, Kelsey ^a   Ganguly, Archan ^b   Roy, Subhojit ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Actin; F actin probes; Lifeact; Utrophin

Indexed keywords

ACTIN;

ACTIN FILAMENT; ANIMAL; AXON; CELL CULTURE; CELL CULTURE TECHNIQUE; CYTOLOGY; DENDRITE; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HIPPOCAMPUS; KYMOGRAPHY; METABOLISM; MOUSE; PHYSIOLOGY; PROCEDURES; STAINING;

ACTIN CYTOSKELETON; ACTINS; ANIMALS; AXONS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; DENDRITES; HIPPOCAMPUS; KYMOGRAPHY; MICE; MICROSCOPY, FLUORESCENCE; STAINING AND LABELING; TRANSFECTION;

EID: 84938153168     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/bs.mcb.2015.07.003     Document Type: Article

References (16)

1. Bearer E.L., Reese T.S. Association of actin filaments with axonal microtubule tracts. Journal of Neurocytology 1999, 28:85-98.
2. Burkel B.M., von Dassow G., Bement W.M. Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Cell Motility and the Cytoskeleton 2007, 64:822-832.
3. Das U., Scott D.A., Ganguly A., Koo E.H., Tang Y., Roy S. Activity-induced convergence of APP and BACE-1 in acidic microdomains via an endocytosis-dependent pathway. Neuron 2013, 79:447-460.
4. Dent E.W., Merriam E.B., Hu X. The dynamic cytoskeleton: backbone of dendritic spine plasticity. Current Opinion in Neurobiology 2011, 21:175-181.
5. Fath K.R., Lasek R.J. Two classes of actin microfilaments are associated with the inner cytoskeleton of axons. Journal of Cell Biology 1988, 107:613-621.
6. Ganguly A., Roy S. Using photoactivatable GFP to track axonal transport kinetics. Methods in Molecular Biology 2014, 1148:203-215.
7. Ganguly A., Tang Y., Wang L., Ladt K., Loi J., Dargent B., et al. A dynamic formin-dependent deep F-actin network in axons. Journal of Cell Biology 2015, 210(3). 10.1083/jcb.201506110.
8. Kaech S., Banker G. Culturing hippocampal neurons. Nature Protocols 2006, 1:2406-2415.
9. Letourneau P.C. Actin in axons: stable scaffolds and dynamic filaments. Results and Problems in Cell Differentiation 2009, 48:65-90.
10. Riedl J., Crevenna A.H., Kessenbrock K., Yu J.H., Neukirchen D., Bista M., et al. Lifeact: a versatile marker to visualize F-actin. Nature Methods 2008, 5:605-607.
11. Roy S., Yang G., Tang Y., Scott D.A. A simple photoactivation and image analysis module for visualizing and analyzing axonal transport with high temporal resolution. Nature Protocols 2011, 7:62-68.
12. Schuh M. An actin-dependent mechanism for long-range vesicle transport. Nature Cell Biology 2011, 13:1431-1436.
13. Scott D.A., Tabarean I., Tang Y., Cartier A., Masliah E., Roy S. A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration. Journal of Neuroscience 2010, 30:8083-8095.
14. Tang Y., Scott D.A., Das U., Edland S.D., Radomski K., Koo E.H., et al. Early and selective impairments in axonal transport kinetics of synaptic cargoes induced by soluble amyloid beta-protein oligomers. Traffic 2012, 13:681-693.
15. Wang L., Das U., Scott D.A., Tang Y., McLean P.J., Roy S. Alpha-synuclein multimers cluster synaptic vesicles and attenuate recycling. Current Biology 2014, 24:2319-2326.
16. Xu K., Zhong G., Zhuang X. Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons. Science 2013, 339:452-456.