Enrichment and identification of δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Data in Brief

Volumn 4, Issue , 2015, Pages 641-649

  Lange, Kerstin ^a,b   Poetsch, Ansgar ^c   Schmid, Andreas ^b   Julsing, Mattijs K ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

^b HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^c RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

YEAST;

CANNABINOIDS; CATIONIC EXCHANGE; CULTURE SUPERNATANT; ESI-MS-MS; MS/MS ANALYSIS; P. PASTORIS; P.PASTORIS; PICHIA PASTORIS; SYNTHASES;

CHROMATOGRAPHY;

EID: 84938120345     PISSN: None     EISSN: 23523409     Source Type: Journal    
DOI: 10.1016/j.dib.2015.07.033     Document Type: DP

References (6)

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2. 9-Tetrahydrocannabinolicacid synthase production in Pichia pastoris enables chemical synthesis of cannabinoids. J. Biotechnol. 2015, 211:68-76.
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
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5. Eng J.K., McCormack A.L., Yates J.R. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J. Am Soc. Mass Spectrom. 1994, 5(11):976-989.
6. Kall L., et al. Posterior error probabilities and false discovery rates: two sides of the same coin. J. Proteome Res. 2008, 7(1):40-44.