A cell-based internalization and degradation assay with an activatable fluorescence-quencher probe as a tool for functional antibody screening

Journal of Biomolecular Screening

Volumn 20, Issue 7, 2015, Pages 869-875

  Li, Yan ^a   Liu, Peter Corbett ^a   Shen, Yang ^a   Snavely, Marshall D ^a   Hiraga, Kaori ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

antibody labeling; antibody screening; cellbased assay; degradation; flow cytometry; fluorescent dye; internalization; quencher dye

Indexed keywords

DYE; FLUORESCENT DYE; QUENCHER DYE; UNCLASSIFIED DRUG; ANTIBODY CONJUGATE; ANTINEOPLASTIC AGENT; MONOCLONAL ANTIBODY;

ANTIBODY LABELING; ARTICLE; CELL ASSAY; CELL CULTURE; ENDOCYTOSIS; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; DRUG SCREENING; FLUORESCENT ANTIBODY TECHNIQUE; HIGH THROUGHPUT SCREENING; METABOLISM; PROCEDURES; PROTEIN TRANSPORT; TUMOR CELL LINE;

ANTIBODIES, MONOCLONAL; ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; DRUG SCREENING ASSAYS, ANTITUMOR; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOCONJUGATES; PROTEIN TRANSPORT; PROTEOLYSIS;

EID: 84937893179     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057115588511     Document Type: Article

References (10)

1. Glassman P. M., Balthasar J. P., Mechanistic Considerations for the Use of Monoclonal Antibodies for Cancer Therapy. Cancer Biol. Med. 2014, 11, 20-33.
2. Sliwkowski M. X., Mellman I., Antibody Therapeutics in Cancer. Science 2013, 341, 1192-1198.
3. Carter P. J., Potent Antibody Therapeutics by Design. Nat. Rev. Immunol. 2006, 6, 343-357.
4. Ritchie M., Tchistiakova L., Scott N., Implications of Receptor-Mediated Endocytosis and Intracellular Trafficking Dynamics in the Development of Antibody Drug Conjugates. mAbs 2013, 5, 13-21.
5. Yarden Y., Agonistic Antibodies Stimulate the Kinase Encoded by the Neu Protooncogene in Living Cells but the Oncogenic Mutant Is Constitutively Active. Proc. Natl. Acad. Sci. USA 1990, 87, 2569-2573.
6. Hurwitz E., Stancovski I., Sela M.,. Suppression and Promotion of Tumor Growth by Monoclonal Antibodies to ErbB-2 Differentially Correlate with Cellular Uptake. Proc. Natl. Acad. Sci. USA 1995, 92, 3353-3357.
7. Poul M. A., Becerril B., Nielsen U. B.,. Selection of Internalizing Human Antibodies from Phage Libraries. J. Mol. Biol. 2000, 301, 1149-1161.
8. Ogawa M., Kosaka N., Longmire M.,. Fluorophore-Quencher Based Activatable Targeted Optical Probes for Detecting In Vivo Cancer Metastases. Mol. Pharm. 2009, 6, 386-395.
9. Peng X., Chen H., Draney D. R.,. A Nonfluorescent, Broad-Range Quencher Dye for Förster Resonance Energy Transfer Assays. Analytical Biochemistry 2009, 388, 220-228.
10. Förster T., Intermolecular Energy Migration and Fluorescence. Ann. Phys. (Leipig). 1948, 2, 55-75.