Cell damage detection using Escherichia coli reporter plasmids: fluorescent and colorimetric assays

Archives of Microbiology

Volumn 197, Issue 6, 2015, Pages 815-821

  Padilla Martínez, Felipe ^a   Carrizosa Villegas, Luz Adriana ^a   Rangel Serrano, Ángeles ^a   Paramo Pérez, Itzel ^a   Mondragón Jaimes, Verónica ^b   Anaya Velázquez, Fernando ^a   Padilla Vaca, Felipe ^a   Franco, Bernardo ^a  

^a UNIVERSITY OF GUANAJUATO   (Mexico)

^b UNIVERSIDAD AUTÓNOMA DE NAYARIT   (Mexico)

Author keywords

Cellular damage; DNA damage; Escherichia coli; Green fluorescent protein; Membrane damage; Oxidative damage; Protein damage; Galactosidase

Indexed keywords

BETA GALACTOSIDASE; CATALASE; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN; MEMBRANE PROTEIN; XENOBIOTIC AGENT; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIAL STRAIN; CELL DAMAGE; COLORIMETRY; CONTROLLED STUDY; DNA DAMAGE; ENZYMATIC ASSAY; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE; FLUOROMETRY; MEMBRANE DAMAGE; NONHUMAN; OXIDATIVE STRESS; PLASMID; POLLUTANT; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FOLDING; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REPORTER GENE; GENETIC PROCEDURES; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROCEDURES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BASE SEQUENCE; BETA-GALACTOSIDASE; BIOSENSING TECHNIQUES; COLORIMETRY; DNA DAMAGE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GREEN FLUORESCENT PROTEINS; OXIDATIVE STRESS; PLASMIDS; PROMOTER REGIONS, GENETIC;

EID: 84937813565     PISSN: 03028933     EISSN: 1432072X     Source Type: Journal    
DOI: 10.1007/s00203-015-1119-y     Document Type: Article

References (21)

1. Bechor O, Smulski DR, Van Dyk TK, LaRossa RA, Belkin S (2002) Recombinant microorganisms as environmental biosensors: pollutants detection by Escherichia coli bearing fabA’: lux fusions. J Biotechnol 94:125–132
2. Belkin S, Smulski DR, Vollmer AC, Van Dyk TK, LaRossa RA (1996) Oxidative stress detection with Escherichia coli harboring a katG’: lux fusion. Appl Environ Microbiol 62:2252–2256
3. Crameri A, Whitehorn EA, Tate E, Stemmer WP (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14(3):315–319
4. Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W (2000) Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem Rev 100(7):2705–2738
5. De Flora S, Camoirano A, Zanacchi P, Bennicelli C (1984) Mutagenicity testing with TA97 and TA102 of 30 DNA-damaging compounds, negative with other Salmonella strains. Mutat Res 134(2–3):159–165
6. Gu M, Mitchell RJ, Kim BC (2004) Whole-cell-based biosensors for environmental biomonitoring and application. Adv Biochem Eng Biotechnol 87:269–305
7. Imlay JA (2013) The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium. Nat Rev Microbiol 11:443–454
8. Jones AM, Grossmann G, Danielson JÅ, Sosso D, Chen LQ, Ho CH, Frommer WB (2013) In vivo biochemistry: applications for small molecule biosensors in plant biology. Curr Opin Plant Biol 16:89–95
9. Kohlmeier S, Mancuso M, Tecon R, Harms H, van der Meer JR, Wells M (2007) Bioreporters: gfp versus lux revisited and single-cell response. Biosens Bioelectron 22:1578–1585
10. Lu C, Albano CR, Bentley WE, Rao G (2005) Quantitative and kinetic study of oxidative stress regulons using green fluorescent protein. Biotechnol Bioeng 89(5):574–587
11. Neidhardt FC, VanBogelen RA (1987) Heat shock response. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, pp 1334–1345
12. Park SC, Lim JY, Jeen YT, Keum B, Seo YS, Kim YS, Lee SJ, Lee HS, Chun HJ, Um SH, Kim CD, Ryu HS, Sul D, Oh E (2012) Ethanol-induced DNA damage and repair-related molecules in human intestinal epithelial Caco-2 cells. Mol Med Rep. 5(4):1027–1032
13. Raut N, O’Connor G, Pasini P, Daunert S (2012) Engineered cells as biosensing systems in biomedical analysis. Anal Bioanal Chem 402:3147–3159
14. Robbens J, Dardenne F, Devriese L, CoenW De, Blust R (2010) Escherichia coli as a bioreporter in ecotoxicology. Appl Microbiol Biotechnol 88:1007–1025
15. Roelant CH, Burns DA, Scheirer W (1996) Accelerating the pace of luciferase reporter gene assays. Biotechniques 20:914–917
16. Sambrook J, Russell DW (eds) (2001) molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, New York
17. Truffer F, Buffi N, Merulla D, Beggah S, van Lintel H, Renaud P, van der Meer JR, Geiser M (2014) Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells. Rev Sci Instrum 85:015120. doi:10.1063/1.4863333
18. Van Dyk TK, Majarian WR, Konstantinov KB, Young RM, Dhurjati PS, LaRossa RA (1994) Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions. Appl Environ Microbiol 60:1414–1420
19. Vangnai AS, Kataoka N, Soonglerdsongpha S, Kalambaheti C, Tajima T, Kato J (2012) Construction and application of an Escherichia coli bioreporter for aniline and chloroaniline detection. J Ind Microbiol Biotechnol 39:1801–1810
20. Vollmer AC, Belkin S, Smulski DR, Van Dyk TK, LaRossa RA (1997) Detection of DNA damage by use of Escherichia coli carrying recA’: lux, uvrA’: lux, or alkA’: lux reporter plasmids. Appl Environ Microbiol 63:2566–2571
21. Wakayama Y, Takagi M, Yano K (1984) Gene responsible for protecting Escherichia coli from sodium dodecyl sulfate and toluidine blue plus light. J Bacteriol 159:527–532