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3
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0026719242
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K. Hamano, M. Kinoshita, K. Furuya, M. Miyamoto, Y. Takamatsu, A. Hemmi, and K. Tanzawa J. Antibiot. 45 1992 899
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(1992)
J. Antibiot.
, vol.45
, pp. 899
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-
Hamano, K.1
Kinoshita, M.2
Furuya, K.3
Miyamoto, M.4
Takamatsu, Y.5
Hemmi, A.6
Tanzawa, K.7
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4
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-
0026683702
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K. Hamano, M. Kinoshita, K. Tanzawa, K. Yoda, Y. Ohki, T. Nakamura, and T. Kinoshita J. Antibiot. 45 1992 906
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(1992)
J. Antibiot.
, vol.45
, pp. 906
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-
Hamano, K.1
Kinoshita, M.2
Tanzawa, K.3
Yoda, K.4
Ohki, Y.5
Nakamura, T.6
Kinoshita, T.7
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5
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84937765685
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note
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4 in water) at 30 °C with shaking at 280 rpm for 7 days. The fermentation broth was centrifuged at 3500 rpm for 5 min to separate the supernatant and cells. The supernatant was loaded to a Diaion HP-20 column directly. The pelleted cells were extracted three times with 500 mL of methanol. After solvent evaporation, the residue was re-suspended in water and loaded to the same Diaion HP-20 column. The column was successively eluted with 0%, 25%, 50%, 75%, and 100% aqueous methanol. The target compounds were eluted by 50% aqueous methanol. This fraction was further separated on an Agilent 1200 HPLC using an Agilent Eclipse XDB C-18 (5 μm, 4.6 mm × 150 mm), eluted with 70% methanol-water at 1 mL/min. The peaks at 19.9 and 27.8 min were collected to yield 1 (110.0 mg) and 2 (11.5 mg) in pure form.
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-
-
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10
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33645515368
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J. Zhan, H. Guo, L. Ning, Y. Zhang, and D. Guo Planta Med. 72 2006 346
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(2006)
Planta Med.
, vol.72
, pp. 346
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Zhan, J.1
Guo, H.2
Ning, L.3
Zhang, Y.4
Guo, D.5
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11
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0037124438
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J. Zhan, H. Guo, J. Dai, Y. Zhang, and D. Guo Tetrahedron Lett. 43 2002 4519
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(2002)
Tetrahedron Lett.
, vol.43
, pp. 4519
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-
Zhan, J.1
Guo, H.2
Dai, J.3
Zhang, Y.4
Guo, D.5
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13
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77950628580
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C. Yu, H. Xu, G. Huang, T. Chen, G. Liu, N. Chai, Y. Ji, S. Wang, Y. Dai, and S. Yuan Appl. Microbiol. Biotechnol. 86 2010 863
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(2010)
Appl. Microbiol. Biotechnol.
, vol.86
, pp. 863
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-
Yu, C.1
Xu, H.2
Huang, G.3
Chen, T.4
Liu, G.5
Chai, N.6
Ji, Y.7
Wang, S.8
Dai, Y.9
Yuan, S.10
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14
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84937765687
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note
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The biotransformation broth of 1 was harvested by centrifugation at 3500 rpm for 5 min. The supernatant was extracted three times with an equal volume of ethyl acetate. The solvent was evaporated in vacuo. The residue was re-dissolved in methanol and separated on an open MCI gel column, eluted with 0%, 25%, 50%, 75%, and 100% aqueous methanol to afford five fractions. The target compounds were eluted with 75% aqueous methanol. This fraction was then further separated on an Agilent 1200 HPLC using an Agilent Eclipse XDB C-18 (5 μm, 4.6 mm × 150 mm), eluted with 50% acetonitrile-water containing 0.05% formic acid at 1 mL/min. The peaks at 6.9, 9.5 and 13.6 min were collected to yield 5 (2.4 mg), 3 (15.4 mg) and 4 (6.5 mg) in pure form.
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15
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0037735190
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J. Zhan, W. Liu, H. Guo, Y. Zhang, and D. Guo Enzyme Microb. Technol. 33 2003 29
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(2003)
Enzyme Microb. Technol.
, vol.33
, pp. 29
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Zhan, J.1
Liu, W.2
Guo, H.3
Zhang, Y.4
Guo, D.5
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16
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84937765688
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note
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Escherichia coli XL-1 Blue, SA ATCC 25923 and MRSA ATCC 33591 were incubated in LB broth with the test samples at different concentrations (125, 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.064 μg/mL) at 37 C in 96-well plates, and the growth was analyzed after 20 h.
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