Deletion of hxk1 gene results in derepression of xylose utilization in Scheffersomyces stipitis

Journal of Industrial Microbiology and Biotechnology

Volumn 42, Issue 6, 2015, Pages 889-896

  Dashtban, Mehdi ^a   Wen, Xin ^a   Bajwa, Paramjit K ^a   Ho, Chi Yip ^b   Lee, Hung ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b MOUNT SINAI HOSPITAL   (Canada)

Author keywords

Glucose repression; Hexokinase; Lignocellulose; Pentose fermenting; Yeast

Indexed keywords

ALCOHOL; GLUCOSE; HEXOKINASE; LIGNIN; LIGNOCELLULOSE; MANNOSE; XYLITOL; XYLOSE;

BIOMASS; BIOSYNTHESIS; CHEMISTRY; DEFICIENCY; ENZYMOLOGY; FERMENTATION; FUNGAL GENE; GENE DELETION; GENETICS; METABOLISM; SACCHAROMYCETALES;

BIOMASS; ETHANOL; FERMENTATION; GENE DELETION; GENES, FUNGAL; GLUCOSE; HEXOKINASE; LIGNIN; MANNOSE; SACCHAROMYCETALES; XYLITOL; XYLOSE;

EID: 84937761024     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-015-1614-9     Document Type: Article

References (21)

1. Bae YH, Kweon DH, Park YC, Seo JH (2014) Deletion of the HXK2 gene in Saccharomyces cerevisiae enables mixed sugar fermentation of glucose and galactose in oxygen-limited conditions. Process Biochem 49:547–553
2. Bajwa PK, Pinel D, Martin VJ, Trevors JT, Lee H (2010) Strain improvement of the pentose-fermenting yeast Pichia stipitis by genome shuffling. J Microbiol Methods 81:179–186
3. Bajwa PK, Shireen T, D’Aoust F, Pinel D, Martin VJJ, Trevors JT, Lee H (2009) Mutants of the pentose-fermenting yeast pichia stipitis with improved tolerance to inhibitors in hardwood spent sulfite liquor. Biotechnol Bioeng 104:892–900
4. Barbosa MDS, Lee H, Collins-Thompson DL (1990) Additive effects of alcohols, their acidic by-products, and temperature on the yeast Pachysolen tannophilus. Appl Environ Microbiol 56:545–550
5. Bicho PA, Runnals PL, Cunningham JD, Lee H (1988) Induction of xylose reductase and xylitol dehydrogenase activities in Pachysolen tannophilus and Pichia stipitis on mixed sugars. Appl Environ Microbiol 54:50–54
6. Entian KD, Frohlich KU (1984) Saccharomyces cerevisiae mutants provide evidence of hexokinase PII as a bifunctional enzyme with catalytic and regulatory domains for triggering carbon catabolite repression. J Bacteriol 158:29–35
7. Goffrini P, Ficarelli A, Ferrero I (1995) Hexokinase activity is affected in mutants of Kluyveromyces lactis resistant to glucose repression. Microbiology 141:441–447
8. Harner NK, Wen X, Bajwa PK, Austin GD, Ho CY, Habash MB, Trevors JT, Lee H (2015) Genetic improvement of native xylose-fermenting yeasts for ethanol production. J Ind Microbiol Biotechnol 42:1–20
9. Jeffries TW, Grigoriev IV, Grimwood J, Laplaza JM, Aerts A, Salamov A, Schmutz J, Lindquist E, Dehal P, Shapiro H, Jin YS, Passoth V, Richardson PM (2007) Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nat Biotechnol 25:319–326
10. Lee H (1992) Reversible inactivation of D-xylose utilization by d-glucose in the pentose-fermenting yeast Pachysolentannophilus. FEMS Microbiol Lett 92:1–4
11. Lobo Z, Maitra PK (1977) Resistance to 2-deoxyglucose in yeast: a direct selection of mutants lacking glucose-phosphorylating enzymes. Mol Genet Genomics 157:297–300
12. Rose MD, Winston F, Hieter P (1990) Methods in yeast genetics—a laboratory course manual. Cold Spring Harbor Laboratory Press, New York
13. Scorer CA, Clare JJ, Mccombie WR, Romanos MA, Sreekrishna K (1994) Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology 12:181–184
14. Slininger PJ, Thompson SR, Weber S, Liu ZL, Moon J (2011) Repression of xylose-specific enzymes by ethanol in Scheffersomyces (Pichia) stipitis and utility of repitching xylose-grown populations to eliminate diauxic lag. Biotechnology Bioengneering 108:1801–1815
15. Sreenath HK, Jeffries TW (1999) 2-Deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis. Appl Biochem Biotechnol 77–79:211–222
16. Webb SR, Lee H (1990) Regulation of D-xylose utilization by hexoses in pentose-fermenting yeasts. Biotechnol Adv 8:685–697
17. Wedlock DN, James AP, Thornton RJ (1989) Glucose-negative mutants of Pachysolen tannophilus. J Gen Microbiol 135:2019–2026
18. Wedlock DN, Thornton RJ (1989) A hexokinase associated with catabolite repression in Pachysolen tannophilus. J Gen Microbiol 135:2013–2018
19. Weirich J, Goffrini P, Kuger P, Ferrero I, Breunig KD (1997) Influence of mutations in hexose-transporter genes on glucose repression in Kluyveromyces lactis. Eur J Biochem 249:248–257
20. Youderian P, Lawes MC, Creighton C, Cook JC, Saier MH (1999) Mutations that confer resistance to 2-deoxyglucose reduce the specific activity of hexokinase from Myxococcus xanthus. J Bacteriol 181:2225–2235
21. Zimmermann FK, Scheel I (1977) Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Genet Genomics 154:75–82