Deletion of the HXK2 gene in Saccharomyces cerevisiae enables mixed sugar fermentation of glucose and galactose in oxygen-limited conditions

Process Biochemistry

Volumn 49, Issue 4, 2014, Pages 547-553

  Bae, Yi Hyun ^a,b   Kweon, Dae Hyuk ^c   Park, Yong Cheol ^d   Seo, Jin Ho ^a,b  

^a Seoul National University   (South Korea)

^b Seoul National University   (South Korea)

^c Sungkyunkwan University   (South Korea)

^d Kookmin University   (South Korea)

Author keywords

Catabolite repression; Ethanol; Marine biomass; Mixed sugar fermentation of glucose and galactose; Saccharomyces cerevisiae

Indexed keywords

BIOETHANOL; BIOSYNTHESIS; ETHANOL; FERMENTATION; OXYGEN; PROTEINS; SUGAR INDUSTRY; TRANSCRIPTION; YEAST;

CATABOLITE REPRESSION; COMPLEX REGULATION; CONSUMPTION RATES; GLUCOSE CONSUMPTION; GLUCOSE DEPLETION; MARINE BIOMASS; MIXED SUGAR FERMENTATION OF GLUCOSE AND GALACTOSE; MOONLIGHTING PROTEINS;

GLUCOSE;

SACCHAROMYCES CEREVISIAE;

EID: 84897917184     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2014.01.030     Document Type: Article

References (32)

1. N. Wei, J. Quarterman, and Y.S. Jin Marine macroalgae: an untapped resource for producing fuels and chemicals Trends Biotechnol 31 2013 70 77
2. K.A. Jung, S.R. Lim, Y.R. Kim, and J.M. Park Potentials of macroalgae as feedstocks for biorefinery Bioresour Technol 135 2013 182 190
3. M. Yanagisawa, K. Nakamura, O. Ariga, and K. Nakasaki Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides Process Biochem 46 2011 2111 2116
4. J.H. Park, J.J. Yoon, H.D. Park, Y.J. Kim, D.J. Lim, and S.H. Kim Feasibility of biohydrogen production from Gelidium amansii Int J Hydrogen Energy 36 2011 13997 14003
5. D.J. Timson Galactose metabolism in Saccharomyces cerevisiae Dyn Biochem Process Biotechnol Mol Biol 1 2007 63 73
6. S. Ostergaard, L. Olsson, M. Johnston, and J. Nielsen Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network Nat Biotechnol 18 2000 1283 1286
7. S. Zaman, S.I. Lippman, X. Zhao, and J.R. Broach How Saccharomyces responds to nutrients Anu Rev Genet 42 2008 27 81
8. J.M. Gancedo Yeast carbon catabolite repression Microbiol Mol Biol Rev 62 1998 334 361
9. F. Moreno, D. Ahuatzi, A. Riera, C.A. Palomino, and P. Herrero Glucose sensing through the Hxk2-dependent signalling pathway Biochem Soc Trans 33 2005 265 268
10. J. Horak, and D.H. Wolf Catabolite inactivation of the galactose transporter in the yeast Saccharomyces cerevisiae: ubiquitination, endocytosis, and degradation in the vacuole J Bacteriol 179 1997 1541 1549
11. A.K. Sil, S. Alam, P. Xin, L. Ma, M. Morgan, and C.M. Lebo et al. The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP Mol Cell Biol 19 1999 7828 7840
12. 13C flux analysis Microb Cell Fact 4 2005 30
13. J. van den Brink, M. Akeroyd, R. van der Hoeven, J.T. Pronk, J.H. De Winde, and P. Daran-Lapujade Energetic limits to metabolic flexibility: responses of Saccharomyces cerevisiae to glucose-galactose transitions Microbiology 155 2009 1340 1350
14. S.J. Ha, Q. Wei, S.R. Kim, J.M. Galazka, J. Cate, and Y.S. Jin Cofermentation of cellobiose and galactose by an engineered Saccharomyces cerevisiae strain Appl Environ Microbiol 77 2011 5822 5825
15. K.S. Lee, M.E. Hong, S.C. Jung, S.J. Ha, B.J. Yu, and J.M. Koo et al. Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering Biotechnol Bioeng 108 2011 621 631
16. C. Bro, S. Knudsen, B. Regenberg, L. Olsson, and J. Nielsen Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering Appl Environ Microbiol 71 2005 6465 6472
17. R.G. Sanchez, B. Hahn-Hägerdal, and M.F. Gorwa-Grauslund PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae Microb Cell Fact 9 2010 40
18. A. Kümmel, J.C. Ewald, S-M. Fendt, S.J. Jol, P. Picotti, and R. Aebersold et al. Differential glucose repression in common yeast strains in response to HXK2 deletion FEMS Yeast Res 10 2010 322 332
19. D. Ahuatzi, A. Riera, R. Peláez, P. Herrero, and F. Moreno Hxk2 regulates the phosphorylation state of Mig1 and therefore its nucleocytoplasmic distribution J Biol Chem 282 2007 4485 4493
20. D. Ahuatzi, P. Herrero, and F. Tamara de la Cera Moreno The glucose-regulated nuclear localization of hexokinase 2 in Saccharomyces cerevisiae is Mig1-dependent J Biol Chem 279 2004 14440 14446
21. L.M. Raamsdonk, J.A. Diderich, A. Kuiper, M. van Gaalen, A.L. Kruckberg, and J.A. Berden et al. Co-consumption of sugars or ethanol and glucose in a Saccharomyces cerevisiae strain deleted in the HXK2 gene Yeast 18 2001 1023 1033
22. 13C-labelled glucose Yeast 21 2004 769 779
23. J.H. De Winde, M. Crauwels, S. Hohmann, J.M. Thevelein, and J. Winderickx Differential requirement of the yeast sugar kinases for sugar sensing inestablishing the catabolite-repressed state Eur J Biochem 241 1996 633 643
24. F.K. Zimmermann, and I. Scheel Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression Mol Gen Genet 154 1977 75 82
25. J. Nikawa, Y. Tsukagoshi, and S. Yamashita Isolation and characterization of two distinct myo-inositol transporter genes of Saccharomyces cerevisiae J Biol Chem 266 1991 11184 11191
26. D. Mumberg, R. Müller, and M. Funk Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds Gene 156 1995 119 122
27. R.S. Sikorski, and P. Hieter A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae Genetics 122 1989 19 27
28. A. Rodriguez, T. de la Cera, P. Herrero, and F. Moreno The hexokinase 2 protein regulates the expression of the GLK1HXK1 and HXK2 genes of Saccharomyces cerevisiae Biochem J 355 2001 625
29. E. Baruffini, P. Goffrini, C. Donnini, and T. Lodi Galactose transport in Kluyveromyces lactis: major role of the glucose permease Hgt1 FEMS Yeast Res 6 2006 1235 1242
30. M.T. Martinez-Pastor, and F. Estruch Sudden depletion of carbon source blocks translation, but not transcription, in the yeast Saccharomyces cerevisiae FEBS Lett 390 1996 319 322
31. P.A. Bicho, P.L. Runnals, J.D. Cunningham, and H. Lee Induction of xylose reductase and xylitol dehydrogenase activities in Pachysolen tannophilus and Pichia stipitis on mixed sugars Appl Environ Microbiol 54 1988 50 54
32. P. Fernández-García, R. Peláez, P. Herrero, and F. Moreno Phosphorylation of yeast hexokinase 2 regulates its nucleocytoplasmic shuttling J Biol Chem 287 2012 42151 42164