A method to simultaneously construct up to 12 differently sized illumina nextera long mate pair libraries with reduced dna input, time, and cost

BioTechniques

Volumn 59, Issue 1, 2015, Pages 42-45

  Heavens, Darren ^a   Accinelli, Gonzalo Garcia ^a   Clavijo, Bernardo ^a   Clark, Matthew Derek ^a  

^a NORWICH RESEARCH PARK   (United Kingdom)

Author keywords

Genome Assembly; Insert Sizes; Long Mate Pair (Lmp) Construction; Wheat

Indexed keywords

GENOMIC DNA; PROTEIN TAG; STREPTAVIDIN; PLANT DNA;

ARTICLE; BACTERIAL CHROMOSOME; CHROMOSOME 3; CHROMOSOME 3B; COST BENEFIT ANALYSIS; DNA DETERMINATION; DNA ISOLATION; DNA LIBRARY; GENE AMPLIFICATION; GENE MAPPING; GENETIC VARIABILITY; LONG MATE PAIR LIBRARY; MEASUREMENT ACCURACY; NEXT GENERATION SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; TRITICUM AESTIVUM; CHEMISTRY; CHROMOSOMAL MAPPING; COST; DNA SEQUENCE; GENE LIBRARY; GENETICS; HIGH THROUGHPUT SEQUENCING; NUCLEIC ACID AMPLIFICATION; PROCEDURES; TIME FACTOR; WHEAT;

TRITICUM AESTIVUM;

CHROMOSOME MAPPING; COSTS AND COST ANALYSIS; DNA, PLANT; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SEQUENCE ANALYSIS, DNA; TIME FACTORS; TRITICUM;

EID: 84937061293     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114310     Document Type: Article

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