Green to red photoconversion of GFP for protein tracking in vivo

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Sattarzadeh, Amirali ^a   Saberianfar, Reza ^b,d   Zipfel, Warren R ^c   Menassa, Rima ^b,d   Hanson, Maureen R ^a  

^a Department of Obstetrics Gynecology   (United States)

^b UNIVERSITY OF WESTERN ONTARIO   (Canada)

^c Cornell University   (United States)

^d AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CONFOCAL MICROSCOPY; DROSOPHILA; GENETICS; GROWTH, DEVELOPMENT AND AGING; LARVA; LIGHT; METABOLISM; MOLECULAR GENETICS; PC12 CELL LINE; PH; RAT; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ANIMALS; DROSOPHILA; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; LARVA; LIGHT; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; PC12 CELLS; RATS; SEQUENCE ALIGNMENT;

EID: 84936125283     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11771     Document Type: Article

References (30)

1. Baker, S. M., Buckheit, R. W., 3rd & Falk, M. M. Green-to-red photoconvertible fluorescent proteins: tracking cell and protein dynamics on standard wide-field mercury arc-based microscopes. BMC Cell Biol. 11, 15 (2010).
2. Terskikh, A. et al. "Fluorescent timer": protein that changes color with time. Science 290, 1585-1588 (2000).
3. Pletnev, S. et al. Orange fluorescent proteins: structural studies of LSSmOrange, PSmOrange and PSmOrange2. PLoS One 9, e99136 (2014).
4. Wachter, R. M., Watkins, J. L. & Kim, H. Mechanistic diversity of red fluorescence acquisition by GFP-like proteins. Biochemistry 49, 7417-7427 (2010).
5. Adam, V., Berardozzi, R., Byrdin, M. & Bourgeois, D. Phototransformable fluorescent proteins: Future challenges. Current Opin. Chem. Biol. 20C, 92-102 (2014).
6. Wiedenmann, J. et al. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA 101, 15905-15910 (2004).
7. Howson, R. et al. Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains. Comp. Funct. Genomics 6, 2-16 (2005).
8. Huh, W. K. et al. Global analysis of protein localization in budding yeast. Nature 425, 686-691 (2003).
9. Morin, X., Daneman, R., Zavortink, M. & Chia, W. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc. Natl. Acad. Sci. USA 98, 15050-15055 (2001).
10. Quinones-Coello, A. T. et al. Exploring strategies for protein trapping in Drosophila. Genetics 175, 1089-1104 (2007).
11. Xiao, Y. L. et al. High throughput generation of promoter reporter (GFP) transgenic lines of low expressing genes in Arabidopsis and analysis of their expression patterns. Plant Meth. 6, 18 (2010).
12. Cutler, S. R., Ehrhardt, D. W., Griffitts, J. S. & Somerville, C. R. Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc. Natl. Acad. Sci. USA 97, 3718-3723 (2000).
13. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
14. Elowitz, M. B., Surette, M. G., Wolf, P. E., Stock, J. & Leibler, S. Photoactivation turns green fluorescent protein red. Current Biol. 7, 809-812 (1997).
15. Matsuda, A. et al. Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP-histones. Plos One 5, e12768 (2010).
16. Bogdanov, A. M. et al. Green fluorescent proteins are light-induced electron donors. Nature Chem. Biol. 5, 459-461 (2009).
17. Saha, R. et al. Light driven ultrafast electron transfer in oxidative redding of Green Fluorescent Proteins. Sci. Reports 3, 1580 (2013).
18. Brejc, K. et al. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. USA 94, 2306-2311 (1997).
19. Kohler, R. H., Zipfel, W. R., Webb, W. W. & Hanson, M. R. The green fluorescent protein as a marker to visualize plant mitochondria in vivo. Plant J. 11, 613-621 (1997).
20. Reichhart, J. M. & Ferrandon, D. Green balancers. Drosophila Information Service 81, 201-202 (1998).
21. Aiken, C. T., Tobin, A. J. & Schweitzer, E. S. A cell-based screen for drugs to treat Huntington's disease. Neurobiol. Disease 16, 546-555 (2004).
22. Schattat, M. H. et al. Differential coloring reveals that plastids do not form networks for exchanging macromolecules. Plant Cell 24, 1465-1477 (2012).
23. Kohler, R. H. & Hanson, M. R. Plastid tubules of higher plants are tissue-specific and developmentally regulated. J. Cell Sci. 113 (Pt 1), 81-89 (2000).
24. Hanson, M. R. & Sattarzadeh, A. Stromules: recent insights into a long neglected feature of plastid morphology and function. Plant Physiol. 155, 1486-1492 (2011).
25. Hanson, M. R. & Sattarzadeh, A. Trafficking of proteins through plastid stromules. Plant Cell 25, 2774-2782 (2013).
26. Kohler, R. H., Cao, J., Zipfel, W. R., Webb, W. W. & Hanson, M. R. Exchange of protein molecules through connections between higher plant plastids. Science 276, 2039-2042 (1997).
27. Nakajima, K., Sena, G., Nawy, T. & Benfey, P. N. Intercellular movement of the putative transcription factor SHR in root patterning. Nature 413, 307-311 (2001).
28. Wu, S., Koizumi, K., Macrae-Crerar, A. & Gallagher, K. L. Assessing the utility of photoswitchable fluorescent proteins for tracking intercellular protein movement in the Arabidopsis root. PLoS One 6, e27536 (2011).
29. Mathur, J. et al. mEosFP-based green-to-red photoconvertible subcellular probes for plants. Plant Physiol. 154, 1573-1587 (2010).
30. Conley, A. J., Joensuu, J. J., Menassa, R. & Brandle, J. E. Induction of protein body formation in plant leaves by elastin-like polypeptide fusions. BMC Biol. 7, 48 (2009).