Codon optimization, expression in Escherichia coli, and immunogenicity of recombinant Chinese sacbrood virus (CSBV) structural proteins VP1, VP2, and VP3

PLoS ONE

Volumn 10, Issue 6, 2015, Pages

  Fei, Dongliang ^a   Zhang, Haochun ^a   Diao, Qingyun ^b   Jiang, Lili ^a   Wang, Qiang ^c   Zhong, Yi ^a   Fan, Zhaobin ^a   Ma, Mingxiao ^a  

^a JINZHOU MEDICAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c Liaoning Water Conservancy Vocational College   (China)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; PROTEIN VP1; PROTEIN VP2; PROTEIN VP3; RECOMBINANT PROTEIN; CAPSID PROTEIN; CODON; IMMUNOGLOBULIN G; VIRUS ANTIBODY; VIRUS ANTIGEN;

ANIMAL EXPERIMENT; ANTIBODY BLOOD LEVEL; ARTICLE; CHINESE SACBROOD VIRUS; CODON; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; IMMUNOGENICITY; LYMPHOCYTE PROLIFERATION; MOUSE; MTT ASSAY; NONHUMAN; PICORNAVIRIDAE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; WESTERN BLOTTING; ANIMAL; BLOOD; GENETICS; IMMUNIZATION; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; PLASMID; RNA VIRUS;

APOIDEA; ESCHERICHIA COLI; IFLAVIRUS; MIRIDAE; MUS; RNA VIRUSES; SACBROOD VIRUS;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CAPSID PROTEINS; CODON; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNIZATION; IMMUNOGLOBULIN G; LYMPHOCYTE ACTIVATION; MICE; PLASMIDS; RECOMBINANT PROTEINS; RNA VIRUSES;

EID: 84936076271     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0128486     Document Type: Article

References (35)

1. Yan X, Han RC (2008) Diagnostic technologies of common pathogens of honeybees in China. Chin Bull Entomol 45: 483-488.
2. Liu X, Zhang Y, Yan X (2010) Prevention of Chinese sacbrood virus infection in Apis cerana using RNA interference. Current microbiology 61: 422-428. doi: 10.1007/s00284-010-9633-2 PMID: 20379718.
3. Runckel C, Flenniken ML, Engel JC, Ruby JG, Ganem D, Andino R, et al. (2011) Temporal analysis of the honeybee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia. PLoS One 6: e20656. doi: 10.1371/journal.pone.0020656 PMID: 21687739.
4. Ai HX, Yan X, Han R (2011) Occurrence and prevalence of seven bee viruses in Apis mellifera and Apis cerana apiaries in China. J Invertebr Pathol 109: 160-164. doi: 10.1016/j.jip.2011.10.006 PMID: 22062807.
5. Feng JX, Zhang QF, Ma ZW, Zhang JQ, Huang WZ, Zhang XF, et al. (1998) Study on the purification, crystallization and structure of Chinese Sacbrood Bee Virus. J Electr Micros (in Chinese) 17: 387-388.
6. Zhang X (2011) Managed honeybee colonies and honey production in China grew during the last five decades. J Apicul Sci 55: 77-85.
7. Ma MX, Li M, Cheng J, Yang S, Wang SD, Lei PF. (2011) Molecular and biological characterization of Chinese sacbrood virus LN isolate. Comp Funct Genomics id: 409386. doi: 10.1155/2011/409386 PMID: 21527980.
8. Bailey L, Gibbs AJ, Woods RD (1964) Sacbrood virus of the larval honey bee (Apis mellifera Linnaeus). Virology 23: 425-429. PMID: 14194138.
9. Xu SF, Zhou T, Hu YY, Song HL, Yang J, Luo QR, et al. (2011) Cloning and phylogeny evolution analysis of sacbrood virus gene CSBV-BJ /2010 in Chinese honey bee (Apis cerana). J Shanghai Jiaotong Universi (Science) 29: 44-48.
10. Ghosh RC, Ball BV, Willcocks MM, Carter MJ (1999) The nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus. J Gen Virol. 80: 1541-1549. PMID: 10374974.
11. Bailey L (1976) Viruses attacking the the honeybee. Adv Virus Res. 20: 271-304. PMID: 775948
12. Freiberg M, De Jong D, Message D, Cox-Foster D (2012) First report of sacbrod virus in honey bee (Apis mellifera) colonies in Brazil. Genet Mol Res 11: 3310-3314. doi: 10.4238/2012 PMID: 23079825.
13. Nguyen NT, Le HL (2013) Complete Genome Sequence of Sacbrood Virus Strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam. Genome Announc 1: e00076-12. doi: 10.1128/genomeA.00076-12 PMID: 23405307.
14. Wang Q, Mei C, Zhen H, Zhu J (2012) Codon Preference Optimization Increases Prokaryotic Cystatin C Expression. J Biomed Biotechnol id: 732017. doi: 10.1155/2012/732017 PMID: 23093857.
15. Welch M, Govindarajan S, Ness JE, Villalobos A, Gurney A, Minshull J, et al. (2009) Design parameters to control synthetic gene expression in Eschorichia coli. PLoS One 4: e7002. doi: 10.1371/journal.pone.0007002 PMID: 19759823.
16. Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM (2014) Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase. Microb Cell Fact 13: 7. doi: 10.1186/1475-2859-13-7 PMID: 24410856.
17. Sun X, Mei M, Zhang X, Han F, Jia B, Wei X, et al. (2014) The extracellular matrix protein mindin as a novel adjuvant elicits stronger immune responses for rBAG1, rSRS4 and rSRS9 antigens of Toxoplasma gondii in BALB/c mice. BMC Infect Dis 14:429-436. doi: 10.1186/1471-s^#2334-14-429 PMID: 25091724.
18. Bhakta G, Nurcombe V, Maitra A, Shrivastava A (2014) DNA-encapsulated magn-esium phosphate nanoparticles elicit both humoral and cellular immune responses in mice. Results Immuol 4:46-53. doi: 10.1016/j.rinim.2014.04.001 PMID: 24936399.
19. Feng M, Fang Y, Han B, Zhang L, Lu X, et al. (2013) Novel aspects of understanding molecular working mechanisms of salivary glands of worker honeybees (Apis mellifera) investigated by proteomics and phosphoproteomics. J Proteomics 87: 1-15. doi: 10.1016/j.jprot.2013.05.021 PMID: 23721798.
20. Ma MX, Li M, Yuan CY, Li PF, Zhang YB (2010) Development of a RT-PCR method for determination of Chinese sacbrood virus. Chin J Biol 23: 425-427.
21. Xia X, Mao Q, Wang H, Zhou B, Wei T (2014) Replication of Chinese sacbrood virus in primary cell cultures of Asian honeybee (Apis cerana). Arch Virol 159: 3435-3438. doi: 10.1007/s00705-014-2183-3 PMID: 25139546.
22. Choe SE, Nguyen LT, Noh JH, Kweon CH, Reddy KE, Koh HB, et al. (2012) Analysis of the complete genome sequence of two Korean sacbrood viruses in the Honey bee, Apis mellifera. Virology 432: 155-161. doi: 10.1016/j.virol.2012.06.008 PMID: 22749880.
23. Ma MX, Liu JH, Song YJ, Li L, Li YF (2013) TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus. PLoS One 8: e52670. doi: 10.1371/journal.pone.0052670 PMID: 23408931.
24. Han B, Zhang L, Feng M, Fang Y, Li J (2013) An integrated proteomics reveals pathological mechanism of honeybee (Apis cerena) sacbrood disease. J Proteome Res 12: 1881-1897. doi: 10.1021/pr301226d PMID: 23418745.
25. Hussmann KL, Vandergaast R, Zheng K, Hoover LI, Fredericksen BL (2014) Structural proteins of West Nile virus are a major determinant of infectious particle production and fitness in astrocytes. J Gen Virol 95: 1991-2003. doi: 10.1099/vir.0.065474-0 PMID: 24920724.
26. Fernández-Sainz IJ, Largo E, Gladue DP, Fletcher P, O'Donnell V, Holinka LG, et al (2014) Effect of specific amino acid substitutions in the putative fusion peptide of structuralglycoprotein E2 on Classical Swine Fever Virus replication. Virology 456-457: 121-130. doi: 10.1016/j.virol.2014.03.005 PMID: 24889231.
27. Zorina VN, Zorin NA (2013) Protein components of innate immunity in protection from pathogenic invasion (literature review). Zh Mikrobiol Epidemiol Immunobiol 3: 111-117. PMID: 24000604.
28. Laguía-Becher M, Martín V, Kraemer M, Corigliano M, Yacono ML, Goldman A, et al. (2010) Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice. BMC Biotechnol 10: 52-76. doi: 10.1186/1472-6750-10-52 PMID: 20633273.
29. Liu YS, Wu CS, Wang JY, Mo W, Yu M (2013) Codon optimization, expression, purification,and functional characterization of recombinant human IL-25 in Pichia pastoris. Appl Microbiol Biotechnol 97: 10349-10358. doi: 10.1007/s00253-013-5264-4 PMID: 24100683.
30. Menzella HG (2011) Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli. Microb Cell Fact 10: 15-23. doi: 10.1186/1475-2859-10-15 PMID: 21371320.
31. Gao JP, Meng CC, Chen ZY, Li CF, Liu GQ (2013) Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells. J Vet Sci 14: 441-447. PMID: 23820215.
32. Bull JC, Ryabov EV, Prince G, Mead A, Zhang C, Baxter LA, et al. (2012) A strong immune response in young adult honeybees masks their increased susceptib-ility to infection comparedto older bees. PLoS Pathog 8: e1003083. doi: 10.1371/journal.ppat.1003083 PMID: 23300441.
33. Evans JD, Aronstein K, Chen YP, Hetru C, Imler JL, Jiang H, et al. (2006) Immune pathways and defence mechanisms in honey bees Apis mellifera. Insect Mol Biol 15: 645-656. PMID: 17069638.
34. Hoffmann JA (2003) The immune response of Drosophila. Nature 426: 33-38. PMID: 14603309.
35. Hultmark D (2003) Drosophila immunity: Paths and patterns. Curr Opin Immunol 15: 12-19. PMID: 12495727.