Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 997, Issue , 2015, Pages 105-109

  Ahirwar, Rajesh ^a,b   Nahar, Pradip ^a,b  

^a CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

^b ACADEMY OF SCIENTIFIC AND INNOVATIVE RESEARCH ACSIR   (India)

Author keywords

Aptamer affinity chromatography; Canavalia ensiformis; Concanavalin A; DNA aptamer; NHS activated agarose

Indexed keywords

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHY;

AGAROSE; APTAMERS; CANAVALIA ENSIFORMIS; CONCANAVALIN A; DNA APTAMER;

PURIFICATION;

AGAROSE; APTAMER; CONCANAVALIN A; DNA; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; APTAMER AFFINITY CHROMATOGRAPHY; ARTICLE; CANAVALIA ENSIFORMIS; CONTROLLED STUDY; DERIVATIZATION; IONIC STRENGTH; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; CANAVALIA; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CANAVALIA ENSIFORMIS;

APTAMERS, NUCLEOTIDE; CANAVALIA; CHROMATOGRAPHY, AFFINITY; CONCANAVALIN A; SODIUM CHLORIDE;

EID: 84935038699     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2015.06.003     Document Type: Article

References (17)

1. Parikh I., Cuatrecasas P. Affinity chromatography, principles and applications. Methods of Protein Separation 1975, 255-276. Springer, US. N. Catsimpoolas (Ed.).
2. Janson J.-C. Protein Purification: Principles, High Resolution Methods, and Applications 2011, John Wiley & Sons, Inc., Hoboken, NJ, USA. Third ed.
3. Moser A.C., Hage D.S. Immunoaffinity chromatography: an introduction to applications and recent developments. Bioanalysis 2010, 2:769-790.
4. Duhita N., Hiwasa-Tanase K., Yoshida S., Ezura H. Single-step purification of native miraculin using immobilized metal-affinity chromatography. J. Agric. Food Chem. 2009, 57:5148-5151.
5. Jayasena S.D. Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 1999, 45:1628-1650.
6. Ni X., Castanares M., Mukherjee A., Lupold S.E. Nucleic acid aptamers: clinical applications and promising new horizons. Curr. Med. Chem. 2011, 18:4206-4214.
7. Tombelli S., Minunni M., Mascini M. Analytical applications of aptamers. Biosens. Bioelectron. 2005, 20:2424-2434.
8. Cho E.J., Lee J.-W., Ellington A.D. Applications of aptamers as sensors. Ann. Rev. Anal. Chem. 2009, 2:241-264.
9. Han B., Zhao C., Yin J., Wang H. High performance aptamer affinity chromatography for single-step selective extraction and screening of basic protein lysozyme. J. Chromatogr. B 2012, 903:112-117.
10. Zhao Q., Wu M., Le X.C., Li X.-F. Applications of aptamer affinity chromatography. Trends Anal. Chem. 2012, 41:46-57.
11. Romig T.S., Bell C., Drolet D.W. Aptamer affinity chromatography combinatorial chemistry applied to protein purification. J. Chromatogr. B 1999, 731:275-284.
12. Pavski V., Le X.C. Detection of human immunodeficiency virus type 1 reverse transcriptase using aptamers as probes in affinity capillary electrophoresis. Anal. Chem. 2001, 73:6070-6076.
13. Berezovski M., Nutiu R., Li Y., Krylov S.N. Affinity analysis of a protein-aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures. Anal. Chem. 2003, 75:1382-1386.
14. Clark S.L., Remcho V.T. Electrochromatographic retention studies on a flavin-binding RNA aptamer sorbent. Anal. Chem. 2003, 75:5692-5696.
15. Ahirwar R., Nahar P. Screening and identification of a DNA aptamer to Concanavalin A and its application in food analysis. J. Agric. Food Chem. 2015, 63(16):4104-4111.
16. Pohleven J., Strukelj B., Kos J. Affinity chromatography of lectins. Affinity Chromatography 2012, 49-74. InTech, ISBN: 978-953-51-0325-7. S. Magdeldin (Ed.).
17. Khan T.A., Husain Q., Naeem A. A novel and inexpensive procedure for the purification of Concanavalin A from jack bean (Canavalia ensiformis) extract. Eur. J. Appl. Sci. 2010, 2:70-76.