Assessing phagocytic clearance of cell death in experimental stroke by ligatable fluorescent probes

Journal of Visualized Experiments

Volumn , Issue 87, 2014, Pages

  Minchew, Candace L ^a,b   Didenko, Vladimir V ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

5'OH DNA breaks; Brain Ischemia; DNA damage; Experimental stroke; Focal brain ischemia; In situ detection; Investigative techniques; Issue 87; Medicine; Molecular probe techniques; Phagocytic clearance; Phagocytosis labeling

Indexed keywords

EID: 84934914732     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51261     Document Type: Article

References (11)

1. Jin, R., Yang, G., Li, G. Inflammatory mechanisms in ischemic stroke: role of inflammatory cells. J Leukoc Biol. 87 (5), 779-89, doi: 10.1189/jlb.1109766, (2010).
2. Schilling, M., Besselmann, M., Müller, M., Strecker, J.K., Ringelstein, E.B., Kiefer, R. Predominant phagocytic activity of resident microglia over hematogenous macrophages following transient focal cerebral ischemia: an investigation using green fluorescent protein transgenic bone marrow chimeric mice. Exp Neurol. 196 (2), 290-7 (2005).
3. Faustino, J.V. et al. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. J Neurosci. 31 (36), 12992-3001, doi: 10.1523/JNEUROSCI.2102-11, (2011).
4. Thored, P. et al. Long term accumulation of microglia with proneurogenic phenotype concomitant with persistent neurogenesis in adult subventricular zone after stroke. Glia. 57 (8), 835-849, doi: 10.1002/glia.20810, (2009).
5. Sierra, A., Abiega, O., Shahraz, A., Neumann, H. Janus-faced microglia: beneficial and detrimental consequences of microglial phagocytosis. Front Cell Neurosci. 7 (6), doi: 10.3389/fncel.2013.00006, (2013).
6. Samejima, K., Earnshaw, W.C. Trashing the genome: Role of nucleases during apoptosis. Nat. Rev. Mol. Cell Biol. 6 (9), 677-688 (2005).
7. Minchew, C.L., Didenko, V.V. Fluorescent probes detecting the phagocytic phase of apoptosis: enzyme-substrate complexes of topoisomerase and DNA. Molecules. 16 (6), 4599-4614, doi: 10.3390/molecules16064599, (2011).
8. Shuman, S. Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I. J. Biol. Chem. 267 (24), 16755-16758 (1992).
9. http://www.perkinelmer.com/CMSResources/Images/44-73609man_TSAFluorescenceSystems.pdf
10. Barcia, C. et al. ROCK/Cdc42-mediated microglial motility and gliapse formation lead to phagocytosis of degenerating dopaminergic neurons in vivo. Sci. Rep. 2, 809, doi: 10.1038/srep00809, (2012).
11. Didenko, V.V., Ngo, H., Minchew, C.L., Boudreaux, D.J., Widmayer, M.A., Baskin, D.S. Visualization of irreparable ischemic damage in brain by selective labeling of double-strand blunt-ended DNA breaks. Mol. Med. 8 (12), 818-823 (2002).