Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis

Methods in Molecular Biology

Volumn 911, Issue , 2012, Pages 569-575

  Zolghadr, Kourosh ^a,b   Gregor, Jacqueline ^b   Leonhardt, Heinrich ^c   Rothbauer, Ulrich ^a,b  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b CHROMOTEK GMBH   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

Antibodies; Apoptosis; Chromobodies; Fluorescent proteins; GFP; HCA assay; High content analysis; Live cell microscopy

Indexed keywords

ANTIGEN; BINDING PROTEIN; LAMIN; LAMIN CHROMOBODY; UNCLASSIFIED DRUG;

ANTIGEN BINDING; ANTIGEN STRUCTURE; APOPTOSIS; ARTICLE; CELL LINE; CONTENT ANALYSIS; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROCESSING;

EID: 84934444389     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-968-6_36     Document Type: Article

References (16)

1. Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35: 495-516
2. Neamati N, Fernandez A, Wright S, Kiefer J, McConkey DJ (1995) Degradation of lamin B1 precedes oligonucleosomal DNA fragmentation in apoptotic thymocytes and isolated thymocyte nuclei. J Immunol 154:3788-3795
3. Oberhammer FA, Hochegger K, Froschl G, Tiefenbacher R, Pavelka M (1994) Chromatin condensation during apoptosis is accompanied by degradation of lamin A+B, without enhanced activation of cdc2 kinase. J Cell Biol 126:827-837
4. Biocca S, Pierandrei-Amaldi P, Cattaneo A (1993) Intracellular expression of anti-p21ras single chain Fv fragments inhibits meiotic maturation of Xenopu s oocytes. Biochem Biophys Res Commun 197:422-427
5. Cardinale A, Lener M, Messina S, Cattaneo A, Biocca S (1998) The mode of action of Y13-259 scFv fragment intracellularly expressed in mammalian cells. FEBS Lett 439:197-202
6. Marasco WA, Chen S, Richardson JH, Ramstedt U, Jones SD (1998) Intracellular antibodies against HIV-1 envelope protein for AIDS gene therapy. Hum Gene Ther 9:1627-1642
7. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB, Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
8. Rothbauer U, Zolghadr K, Muyldermans S, Schepers A, Cardoso MC, Leonhardt H (2008) A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins. Mol Cell Proteomics 7:282-289
9. Muyldermans S, Baral TN, Retamozzo VC, De Baetselier P, De Genst E, Kinne J, Leonhardt H, Magez S, Nguyen VK, Revets H, Rothbauer U, Stijlemans B, Tillib S, Wernery U, Wyns L, Hassanzadeh-Ghassabeh G, Saerens D (2009) Camelid immunoglobulins and nanobody technology. Vet Immunol Immunopathol 128: 178-183
10. Olichon A, Schweizer D, Muyldermans S, de Marco A (2007) Heating as a rapid puri fication method for recovering correctly-folded ther-motolerant VH and VHH domains. BMC Biotechnol 7:7
11. Sheriff S, Chang CY, Jeffrey PD, Bajorath J (1996) X-ray structure of the uncomplexed anti-tumor antibody BR96 and comparison with its antigen-bound form. J Mol Biol 259:938-946
12. van der Linden RH, Frenken LG, de Geus B, Harmsen MM, Ruuls RC, Stok W, de Ron L, Wilson S, Davis P, Verrips CT (1999) Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochim Biophys Acta 1431:37-46
13. Rothbauer U, Zolghadr K, Tillib S, Nowak D, Schermelleh L, Gahl A, Backmann N, Conrath K, Muyldermans S, Cardoso MC, Leonhardt H (2006) Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods 3:887-889
14. Susin SA, Daugas E, Ravagnan L, Samejima K, Zamzami N, Loeffler M, Costantini P, Ferri KF, Irinopoulou T, Prevost MC, Brothers G, Mak TW, Penninger J, Earnshaw WC, Kroemer G (2000) Two distinct pathways leading to nuclear apoptosis. J Exp Med 192:571-580
15. Kihlmark M, Imreh G, Hallberg E (2001) Sequential degradation of proteins from the nuclear envelope during apoptosis. J Cell Sci 114:3643-3653
16. Chae HJ, Kang JS, Byun JO, Han KS, Kim DU, Oh SM, Kim HM, Chae SW, Kim HR (2000) Molecular mechanism of staurosporine-induced apoptosis in osteoblasts. Pharmacol Res 42:373-381