Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in arabidopsis and rice

Methods in Molecular Biology

Volumn 913, Issue , 2012, Pages 265-276

  Evrard, Aurelie ^a   Bargmann, Bastiaan O R ^b   Birnbaum, Kenneth D ^b   Tester, Mark ^c   Baumann, Ute ^a   Johnson, Alexander A T ^d  

^a UNIVERSITY OF ADELAIDE   (Australia)

^b NEW YORK UNIVERSITY   (United States)

^c Australian Centre for Plant Functional Genomics   (Australia)

^d UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Arabidopsis; Cell type specific; FACS; GAL4; Protoplast; Rice; Salinity

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ARABIDOPSIS; ARTICLE; CELL TYPE; FLUORESCENCE ACTIVATED CELL SORTING; GERMINATION; NONHUMAN; PLANT GROWTH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTOPLAST; RICE; SALT STRESS;

EID: 84934442373     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-986-0_18     Document Type: Article

References (14)

1. Birnbaum K, et al. (2003) A gene expression map of the Arabidopsis root. Science 302:1956-1960
2. Brady SM, et al. (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318:801-806
3. Dinneny JR, et al. (2008) Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320:942-945
4. Gifford ML, et al. (2008) Cell-specific nitrogen responses mediate developmental plasticity. Proc Natl Acad Sci USA 105:803-808
5. Nawy T, et al. (2005) Transcriptional profile of the Arabidopsis root quiescent center. Plant Cell 17:1908-1925
6. Borges F, et al. (2008) Comparative transcriptomics of Arabidopsis sperm cells. Plant Physiol 148:1168-1181
7. Zhang C, et al. (2008) Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiol 147:30-40
8. Birnbaum K, et al. (2005) Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines. Nat Methods 2:615-619
9. Bargmann BOR, Birnbaum KD (2010) Fluorescence activated cell sorting of plant protoplasts. J Vis Exp (36):e1673
10. Haseloff J (1999) GFP variants for multispectral imaging of living cells. Methods Cell Biol 58:139-151
11. Johnson AAT, et al. (2005) Spatial control of transgene expression in rice ( Oryza sativa L.) using the GAL4 enhancer trapping system. Plant J 41:779-789
12. + transport in Arabidopsis . Plant Cell 21:2163-2178
13. Plett D, et al. (2010) Improved salinity tolerance of rice through cell type-specific expression of AtHKT1;1. PLoS One 5:e12571
14. + transport in higher plants. Ann Bot 91:503-527