Detecting cAMP with an EPAC-based FRET sensor in single living cells

Methods in Molecular Biology

Volumn 1071, Issue , 2014, Pages 49-58

  Klarenbeek, J ^a   Jalink, Kees ^b  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Biosensor; cAMP; EPAC; FRET; Live cell recording

Indexed keywords

ANTIPORTER; CYCLIC AMP;

ARTICLE; CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC TRANSFECTION; PRIORITY JOURNAL; SECOND MESSENGER; SINGLE LIVING CELL;

BIOSENSING TECHNIQUES; CELL SURVIVAL; CYCLIC AMP; FLUORESCENCE RESONANCE ENERGY TRANSFER; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HEK293 CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; SINGLE-CELL ANALYSIS;

EID: 84934441548     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-622-1_4     Document Type: Article

References (16)

1. Gilman AG (1995) Nobel lecture. G proteins and regulation of adenylyl cyclase. Biosci Rep 15:65-97
2. de Rooij J, Zwartkruis FJ, Verheijen MH, Cool RH, Nijman SM et al (1998) Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 396:474-477
3. Nikolaev VO, Bunemann M, Hein L, Hannawacker A, Lohse MJ (2004) Novel single chain cAMP sensors for receptor-induced signal propagation. J Biol Chem 279:37215-37218
4. van der Krogt GN, Ogink J, Ponsioen B, Jalink K (2008) A comparison of donor-acceptor pairs for genetically encoded FRET sensors: application to the Epac cAMP sensor as an example. PLoS One 3:e1916
5. Adams SR, Harootunian AT, Buechler YJ, Taylor SS, Tsien RY (1991) Fluorescence ratio imaging of cyclic AMP in single cells. Nature 349:694-697
6. Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G et al (2004) Detecting cAMPinduced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator. EMBO Rep 5:1176-1180
7. Zaccolo M, De Giorgi F, Cho CY, Feng L, Knapp T et al (2000) A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nat Cell Biol 2:25-29
8. DiPilato LM, Cheng X, Zhang J (2004) Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments. Proc Natl Acad Sci U S A 101:16513-16518
9. Klarenbeek JB, Goedhart J, Hink MA, Gadella TW, Jalink K (2011) A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range. PLoS One 6:e19170
10. Rehmann H, Wittinghofer A, Bos JL (2007) Capturing cyclic nucleotides in action: snapshots from crystallographic studies. Nat Rev Mol Cell Biol 8:63-73
11. Salonikidis PS, Zeug A, Kobe F, Ponimaskin E, Richter DW (2008) Quantitative measurement of cAMP concentration using an exchange protein directly activated by a cAMP-based FRETsensor. Biophys J 95:5412-5423
12. Salonikidis PS, Niebert M, Ullrich T, Bao G, Zeug A et al (2011) An ion-insensitive cAMP biosensor for long term quantitative ratiometric fluorescence resonance energy transfer (FRET) measurements under variable physiological conditions. J Biol Chem 286: 23419-23431
13. Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K et al (2010) Bright cyan fluorescent protein variants identifi ed by fluorescence lifetime screening. Nat Methods 7:137-139
14. Honda A, Sawyer CL, Cawley SM, Dostmann WR (2005) Cygnets: in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP. Methods Mol Biol 307: 27-43
15. Niino Y, Hotta K, Oka K (2010) Blue fluorescent cGMP sensor for multiparameter fluorescence imaging. PLoS One 5:e9164
16. Russwurm M, Mullershausen F, Friebe A, Jager R, Russwurm C et al (2007) Design of fluorescence resonance energy transfer (FRET)-based cGMP indicators: a systematic approach. Biochem J 407:69-77