Engineering adenovirus genome by Bacterial Artificial Chromosome (BAC) technology

Methods in Molecular Biology

Volumn 1089, Issue , 2014, Pages 143-158

  Ruzsics, Zsolt ^a   Lemnitzer, Frederic ^a   Thirion, Christian ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b SIRION BIOTECH GMBH   (Germany)

Author keywords

Bacterial artificial chromosome; Flp FRT recombination; Homologous recombination in E. coli; Recombinant adenoviruses; Recombineering; Red recombinases

Indexed keywords

DNA FRAGMENT;

ADENOVIRUS; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; DNA SEQUENCE; DNA TEMPLATE; DNA VIRUS; GENE SYNTHESIS; GENETIC ENGINEERING; GENETIC MANIPULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RESTRICTION MAPPING; VIRUS GENOME;

ADENOVIRIDAE; BACTERIA (MICROORGANISMS); DNA VIRUSES; ESCHERICHIA COLI; HERPES;

ADENOVIRUSES, HUMAN; CHROMOSOMES, ARTIFICIAL, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, VIRAL; HEK293 CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED; RECOMBINATION, GENETIC;

EID: 84934440723     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-679-5_11     Document Type: Article

References (15)

1. Ruzsics Z, Wagner M, Osterlehner A, Cook J, Koszinowski U, Burgert HG (2006) Transposon-assisted cloning and traceless mutagenesis of adenoviruses: development of a novel vector based on species D. J Virol 80:8100-8113
2. Ruzsics Z, Koszinowski UH (2008) Mutagenesis of the cytomegalovirus genome. Curr Top Microbiol Immunol 325:41-61
3. Sirena D, Ruzsics Z, Schaffner W, Greber UF, Hemmi S (2005) The nucleotide sequence and a fi rst generation gene transfer vector of species B human adenovirus serotype Virology 343:283-298.
4. Imelli N, Ruzsics Z, Puntener D, Gastaldelli M, Greber UF (2009) Genetic reconstitution of the human adenovirus type 2 temperaturesensitive 1 mutant defective in endosomal escape. Virol J 6:174
5. Puntener D, Engelke MF, Ruzsics Z, Strunze S, Wilhelm C, Greber UF (2011) Stepwise loss of fl uorescent core protein V from human adenovirus during entry into cells. J Virol 85:481-496
6. Wodrich H, Henaff D, Jammart B, Segura-Morales C, Seelmeir S, Coux O et al (2010) A capsid-encoded PPxY-motif facilitates adenovirus entry. PLoS Pathog 6:e1000808
7. Jager L, Hausl MA, Rauschhuber C, Wolf NM, Kay MA, Ehrhardt A (2009) A rapid protocol for construction and production of highcapacity adenoviral vectors. Nat Protoc 4:547-564
8. Warming S, Costantino N, Court DL, Jenkins NA, Copeland NG (2005) Simple and highly effi cient BAC recombineering using galK selection. Nucleic Acids Res 33:e36
9. Alper MD, Ames BN (1975) Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions. J Bacteriol 121:259-266
10. Lemnitzer F, Raschbichler V, Kolodziejczak D, Israel L, Imhof A, Bailer SM et al (2013) Mouse cytomegalovirus egress protein pM50 interacts with cellular endophilin-A2. Cell Microbiol 15:335-351
11. Bubeck A, Wagner M, Ruzsics Z, Lotzerich M, Iglesias M, Singh IR et al (2004) Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replication. J Virol 78:8026-8035
12. Wagner M, Koszinowski UH (2004) Mutagenesis of viral BACs with linear PCR fragments (ET recombination). Methods Mol Biol 256:257-268
13. Cherepanov PP, Wackernagel W (1995) Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158:9-14
14. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Colb Spring Harbor LAboratory Press, Cold Spring Harbor, New York
15. Stalker DM, Kolter R, Helinski DR (1979) Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K. Proc Natl Acad Sci USA 76:1150-1154