Imaging kinase activity at protein scaffolds

Methods in Molecular Biology

Volumn 1071, Issue , 2014, Pages 129-137

  Kunkel, Maya T ^a   Newton, Alexandra C ^a  

^a San Diego   (United States)

Author keywords

DKAR; FRET; Kinase activity reporter; NHERF; Protein kinase D; Scaffold protein

Indexed keywords

PHOSPHOTRANSFERASE; PROTEIN KINASE D;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CONTROLLED STUDY; DATA ANALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYMIC BIOSENSOR; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOLECULAR IMAGING; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CELL SURVIVAL; DOGS; ENZYME ASSAYS; FLUORESCENCE RESONANCE ENERGY TRANSFER; MADIN DARBY CANINE KIDNEY CELLS; MOLECULAR IMAGING; PROTEIN KINASES; PROTEIN STRUCTURE, TERTIARY;

EID: 84934439664     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-622-1_10     Document Type: Article

References (17)

1. Toker A (2005) The biology and biochemistry of diacylglycerol signalling. Meeting on molecular advances in diacylglycerol signalling. EMBO Rep 6(4):310-314
2. Sanchez-Ruiloba L, Cabrera-Poch N, Rodriguez-Martinez M, Lopez-Menendez C, Jean-Mairet RM, Higuero AM, Iglesias T (2006) Protein kinase D intracellular localization and activity control kinase D-interacting substrate of 220-kDa traffi c through a postsynaptic density-95/discs large/zonula occludens-1-binding motif. J Biol Chem 281(27): 18888-18900
3. Rozengurt E (2011) Protein kinase D signaling: multiple biological functions in health and disease. Physiology 26(1):23-33
4. Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274(37): 26543-26549
5. Kunkel MT, Newton AC (2009) Spatiotemporal dynamics of kinase signaling visualized by targeted reporters. Curr Protoc Chem Biol 1(1):17-18
6. Sato M, Kawai Y, Umezawa Y (2007) Genetically encoded fluorescent indicators to visualize protein phosphorylation by extracellular signal-regulated kinase in single living cells. Anal Chem 79(6):2570-2575
7. Ting AY, Kain KH, Klemke RL, Tsien RY (2001) Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells. Proc Natl Acad Sci U S A 98(26): 15003-15008
8. Zhang J, Ma Y, Taylor SS, Tsien RY (2001) Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc Natl Acad Sci U S A 98(26): 14997-15002
9. Zhang J, Hupfeld CJ, Taylor SS, Olefsky JM, Tsien RY (2005) Insulin disrupts betaadrenergic signalling to protein kinase A in adipocytes. Nature 437(7058):569-573
10. Allen MD, Zhang J (2006) Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters. Biochem Biophys Res Commun 348(2):716-721
11. Sasaki K, Sato M, Umezawa Y (2003) Fluorescent indicators for Akt/protein kinase B and dynamics of Akt activity visualized in living cells. J Biol Chem 278(33):30945-30951
12. Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC (2005) Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem 280(7):5581-5587
13. Gao X, Zhang J (2008) Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomains. Mol Biol Cell 19(10):4366-4373
14. Violin JD, Zhang J, Tsien RY, Newton AC (2003) A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol 161(5):899-909
15. Kajimoto T, Sawamura S, Tohyama Y, Mori Y, Newton AC (2010) Protein kinase C {delta}-specifi c activity reporter reveals agonist-evoked nuclear activity controlled by Src family of kinases. J Biol Chem 285(53):41896-41910
16. Kunkel MT, Toker A, Tsien RY, Newton AC (2007) Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. J Biol Chem 282(9): 6733-6742
17. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC (2009) The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. J Biol Chem 284(36):24653-24661