Imaging metastatic cell traffi cking at the cellular level in vivo with fluorescent proteins

Methods in Molecular Biology

Volumn 1070, Issue , 2014, Pages 171-179

  Hoffman, Robert M ^a  

^a ANTICANCER INC   (United States)

Author keywords

Blood vessels; Cancer cell trafficking; Green fluorescent protein; In vivo imaging; Lymphatic vessels; Metastasis; Nuclear cytoplasmic dynamic imaging; Red fluorescent protein

Indexed keywords

GREEN FLUORESCENT PROTEIN; HISTONE H2B; RED FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER CELL; CELL MIGRATION; CELL NUCLEUS; CELL VIABILITY; CYTOPLASM; EXTRAVASATION; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; IN VIVO STUDY; MOUSE; NONHUMAN; NUCLEAR SHAPE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

MUS;

ANIMALS; CELL LINE, TUMOR; CELL MOVEMENT; COMPUTER SYSTEMS; DIAGNOSTIC IMAGING; GREEN FLUORESCENT PROTEINS; HISTONES; HUMANS; LUMINESCENT PROTEINS; MICE; MICE, NUDE; NEOPLASM METASTASIS; STAINING AND LABELING; TRANSDUCTION, GENETIC;

EID: 84934439095     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4614-8244-4_12     Document Type: Article

References (24)

1. Hoffman RM (2003) Green fl uorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models. Lancet Oncol 3:546-556
2. Condeelis J, Segall JE (2003) Intravital imaging of cell movement in tumours. Nat Rev Cancer 3:921-930
3. Chishima T, Miyagi Y, Wang X et al (1997) Cancer invasion and micrometastasis visualised in live tissue by green fl uorescent protein expression. Cancer Res 57:2042-2047
4. Farina KL, Wyckoff JB, Rivera J et al (1998) Cell motility of tumor cells visualised in living intact primary tumors using green fl uorescent protein. Cancer Res 58:2528-2532
5. Yamauchi K, Yang M, Jiang P et al (2005) Real-time in vivo dual-colour imaging of intracapillary cancer cell and nucleus deformation and migration. Cancer Res 65: 4246-4252
6. Huang MS, Wang TJ, Liang CL et al (2002) Establishment of fl uorescent lung carcinoma metastasis model and its real-time microscopic detection in SCID mice. Clin Exp Metastasis 19:359-368
7. Wyckoff JB, Jones JG, Condeelis JS, Segall JEA (2000) A critical step in metastasis: in vivo analysis of intra vasation at the primary tumor. Cancer Res 60:2504-2511
8. Naumov GN, Wilson SM, MacDonald IC et al (1999) Cellular expression of green fl uorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis. J Cell Sci 112:1835-1842
9. Mook ORF, Marle JV, Vreeling-Sindelarova H (2003) Visualisation of early events in tumor formation of eGFP-transfected rat colon cancer cells in liver. Hepatology 38:295-304
10. Al-Mehdi AB, Tozawa K, Fisher AB, Shientag L, Lee A, Muschel RJ (2000) Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis. Nat Med 6:100-102
11. Wang W, Wyckoff JB, Frohlich VC et al (2002) Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profi ling. Cancer Res 62:6278-6288
12. Goswami S, Sahai E, Wyckoff JB et al (2005) Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop. Cancer Res 65:5278-5283
13. Yamamoto N, Jiang P, Yang M et al (2004) Cellular dynamics visualised in live cells in vitro and in vivo by differential dual-colour nuclearcytoplasmic fl uorescent-protein expression. Cancer Res 64:4251-4256
14. Flach J, Bossie M, Bogel J et al (1994) A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Mol Cell Biol 14:8399-8407
15. Kanda T, Sullivan KF, Wahl GM (1998) Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 8:377-385
16. Manders EM, Visser AE, Koppen A et al (2003) Four-dimensional imaging of chromatin dynamics during the assembly of the interphase nucleus. Chromosome Res 11: 537-547
17. Hayashi K, Jiang P, Yamauchi K et al (2007) Real-time imaging of tumor-cell shedding and traffi cing in lymphatic channels. Cancer Res 67:8223-8228
18. Kimura H, Hayashi K, Yamauchi K et al (2010) Real-time imaging of single cancer-cell dynamics of lung metastasis. J Cell Biochem 109(1):58-64
19. Yamauchi K, Yang M, Jiang P et al (2006) Development of real-time subcellular dynamic multicolor imaging of cancer-cell traffi cing in live mice with a variable-magnifi cation wholemouse imaging system. Cancer Res 66:4208-4214
20. Rashidi B, Moossa AR, Hoffman RM (2013) Specifi c route mapping visualized with GFP of single-fi le streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J Cell Biochem 114:1738-1743
21. Hoffman RM (2005) The multiple uses of fl uorescent proteins to visualize cancer in vivo. Nat Rev Cancer 5:796-806
22. Hoffman RM, Yang M (2006) Subcellular imaging in the live mouse. Nat Protoc 1: 775-782
23. Hoffman RM, Yang M (2006) Color-coded fl uorescence imaging og tumor-host interactions. Nat Protoc 1:928-935
24. Hoffman RM, Yang M (2006) Whole-body imaging with fl uorescent proteins. Nat Protoc 1:1429-1438