Isolation of endothelial cells from human tumors

Methods in Molecular Biology

Volumn 731, Issue , 2011, Pages 209-218

  Naschberger, Elisabeth ^a   Schellerer, Vera S ^a   Rau, Tilman T ^a   Croner, Roland S ^a   Stürzl, Michael ^a  

^a NONE

Author keywords

Angiogenesis; CD31; Collagenase II; Colon; Colorectal carcinoma; Endothelial cells; MACS; Tumor

Indexed keywords

CD31 ANTIGEN; ENDOGLIN; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; INTERCELLULAR ADHESION MOLECULE 1; LOW DENSITY LIPOPROTEIN; VASCULAR CELL ADHESION MOLECULE 1; VASCULAR ENDOTHELIAL CADHERIN; VON WILLEBRAND FACTOR; ANGIOGENESIS INHIBITOR;

ANTIGEN EXPRESSION; ARTICLE; CAPILLARY BASEMENT MEMBRANE; CELL DIFFERENTIATION; CELL ISOLATION; CELL SELECTION; CLINICAL PROTOCOL; COLORECTAL CANCER; CONTROLLED STUDY; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; PRIORITY JOURNAL; TISSUE CULTURE; CELL SEPARATION; CELL SURVIVAL; COLON; COLON TUMOR; CYTOLOGY; DRUG EFFECT; MAGNETISM; METHODOLOGY; NEOVASCULARIZATION (PATHOLOGY); PATHOLOGY; SUSPENSION; TRANSPLANTATION; VASCULARIZATION;

ANGIOGENESIS INHIBITORS; CELL SEPARATION; CELL SURVIVAL; COLON; COLONIC NEOPLASMS; ENDOTHELIAL CELLS; HUMANS; MAGNETICS; NEOVASCULARIZATION, PATHOLOGIC; SUSPENSIONS; TISSUE AND ORGAN HARVESTING;

EID: 84934439021     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-80-5_18     Document Type: Article

References (15)

1. Quesada, A. R., Munoz-Chapuli, R., and Medina, M. A. (2006) Anti-angiogenic drugs: from bench to clinical trials, Med Res Rev. 26, 483-530
2. Jain, R. K. (2005) Normalization of tumor vasculature: an emerging concept in antian-giogenic therapy, Science 307, 58-62.
3. Folkman, J. (1971) Tumor angiogenesis: therapeutic implications, N Engl J Med 285, 1182-1186.
4. Folkman, J. (2006) Angiogenesis, Annu Rev Med 57, 1-18.
5. Carmeliet, P. (2005) VEGF as a key mediator of angiogenesis in cancer, Oncology 69 Suppl 3, 4-10.
6. Zanetta, L., Marcus, S. G., Vasile, J., Dobryansky, M., Cohen, H., Eng, K., Shamamian, P., and Mignatti, P. (2000) Expression of Von Willebrand factor, an endothelial cell marker, is up-regulated by angiogenesis factors: a potential method for objective assessment of tumor angiogenesis, Int J Cancer 85, 281-288.
7. Klagsbrun, M. (1992) Mediators of angiogene-sis: the biological signifcance of basic fbroblast growth factor (bFGF)-heparin and heparan sul-fate interactions, Semin Cancer Biol 3, 81-87.
8. Jain, R. K. (2001) Normalizing tumor vascu-lature with anti-angiogenic therapy: A new paradigm for combination therapy, Nat Med 7, 987-989.
9. Jain, R. K. (2003) Molecular regulation of vessel maturation, Nat Med 9, 685-693.
10. St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., and Kinzler, K. W. (2000) Genes expressed in human tumor endothe-lium, Science 289, 1197-1202.
11. Hida, K., and Klagsbrun, M. (2005) A new perspective on tumor endothelial cells: unexpected chromosome and centrosome abnormalities, Cancer Res 65, 2507-2510.
12. Saunders, M., and Iveson, T. (2006) Management of advanced colorectal cancer: state of the art, Br J Cancer 95, 131-138.
13. Hurwitz, H., and Kabbinavar, F. (2005) Bevacizumab combined with standard fuoro-pyrimidine-based chemotherapy regimens to treat colorectal cancer, Oncology 69 Suppl 3, 17-24.
14. Hurwitz, H., Fehrenbacher, L., Novotny, W., Cartwright, T., Hainsworth, J., Heim, W., Berlin, J., Baron, A., Griffing, S., Holmgren, E., Ferrara, N., Fyfe, G., Rogers, B., Ross, R., and Kabbinavar, F. (2004) Bevacizumab plus irinotecan, fluo-rouracil, and leucovorin for metastatic col-orectal cancer, N Engl J Med 350, 2335-2342.
15. Schellerer, V. S., Croner, R. S., Weinländer, K., Hohenberger, W., Stürzl, M., and Naschberger, E. (2007) Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture, Lab Invest 87, 1159-1170.